Understanding missing proteins : a functional perspective
A missing protein (MP) is an unconfirmed genetic sequence for which a protein product is not yet detected. Currently, MPs are tiered based on supporting evidence mainly in the form of protein existence (PE) classification. As we discuss here, this definition is overly restrictive because proteins go...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2020
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| Online Access: | https://hdl.handle.net/10356/144226 |
| _version_ | 1826129830337314816 |
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| author | Zhou, Longjian Wong, Limsoon Goh, Wilson Wen Bin |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Zhou, Longjian Wong, Limsoon Goh, Wilson Wen Bin |
| author_sort | Zhou, Longjian |
| collection | NTU |
| description | A missing protein (MP) is an unconfirmed genetic sequence for which a protein product is not yet detected. Currently, MPs are tiered based on supporting evidence mainly in the form of protein existence (PE) classification. As we discuss here, this definition is overly restrictive because proteins go missing in day-to-day proteomics as a result of low abundance, lack of sequence specificity, splice variants, and so on. Thus, we propose a broader functional classification of MPs that complements PE classification, discuss major causes, and examine three corresponding solution tiers: biological, technical, and informatics. We assert that informatics-driven solutions would have a major role in resolving the MP problem (MPP). |
| first_indexed | 2024-10-01T07:46:37Z |
| format | Journal Article |
| id | ntu-10356/144226 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:46:37Z |
| publishDate | 2020 |
| record_format | dspace |
| spelling | ntu-10356/1442262020-10-21T06:43:18Z Understanding missing proteins : a functional perspective Zhou, Longjian Wong, Limsoon Goh, Wilson Wen Bin School of Biological Sciences Science::Biological sciences Amino Acid Sequence Protein Analysis A missing protein (MP) is an unconfirmed genetic sequence for which a protein product is not yet detected. Currently, MPs are tiered based on supporting evidence mainly in the form of protein existence (PE) classification. As we discuss here, this definition is overly restrictive because proteins go missing in day-to-day proteomics as a result of low abundance, lack of sequence specificity, splice variants, and so on. Thus, we propose a broader functional classification of MPs that complements PE classification, discuss major causes, and examine three corresponding solution tiers: biological, technical, and informatics. We assert that informatics-driven solutions would have a major role in resolving the MP problem (MPP). 2020-10-21T06:43:18Z 2020-10-21T06:43:18Z 2018 Journal Article Zhou, L., Wong, L., & Goh, W. W. B. (2018). Understanding missing proteins : a functional perspective. Drug Discovery Today, 23(3), 644-651. doi:10.1016/j.drudis.2017.11.011 1878-5832 https://hdl.handle.net/10356/144226 10.1016/j.drudis.2017.11.011 29158195 3 23 644 651 en Drug Discovery Today © 2017 Elsevier Ltd. All rights reserved. |
| spellingShingle | Science::Biological sciences Amino Acid Sequence Protein Analysis Zhou, Longjian Wong, Limsoon Goh, Wilson Wen Bin Understanding missing proteins : a functional perspective |
| title | Understanding missing proteins : a functional perspective |
| title_full | Understanding missing proteins : a functional perspective |
| title_fullStr | Understanding missing proteins : a functional perspective |
| title_full_unstemmed | Understanding missing proteins : a functional perspective |
| title_short | Understanding missing proteins : a functional perspective |
| title_sort | understanding missing proteins a functional perspective |
| topic | Science::Biological sciences Amino Acid Sequence Protein Analysis |
| url | https://hdl.handle.net/10356/144226 |
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