Autofluorescence lifetime microscopy in normal and cancer cells and nanosecond pulsed electric field effects
Electric field effects on dynamics and function of biological systems depend on the cell membrane capacitance and on the pulse duration of the applied electric field, which enables selective application of pulsed electric field in specific organelles and specific living cells. If electric field whos...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Conference Paper |
| Language: | English |
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2020
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/144287 |
| _version_ | 1811683802968227840 |
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| author | Awasthi, Kamlesh Change, Feng-Lin Hsu, Hsin-Yun Hsieh, Pei-Ying Ohta, Nobuhiro |
| author2 | Asian Spectroscopy Conference 2020 |
| author_facet | Asian Spectroscopy Conference 2020 Awasthi, Kamlesh Change, Feng-Lin Hsu, Hsin-Yun Hsieh, Pei-Ying Ohta, Nobuhiro |
| author_sort | Awasthi, Kamlesh |
| collection | NTU |
| description | Electric field effects on dynamics and function of biological systems depend on the cell membrane capacitance and on the pulse duration of the applied electric field, which enables selective application of pulsed electric field in specific organelles and specific living cells. If electric field whose pulse width is shorter than the charging time of the outer membrane is applied to living systems, the pulsed electric field may penetrate into the cell and induce changes in intracellular function. In fact, we reported the apoptosis induced by application of nanosecond pulsed electric field (nsPEF) with a pulse width of 10–50 ns in HeLa cells.1 |
| first_indexed | 2024-10-01T04:18:32Z |
| format | Conference Paper |
| id | ntu-10356/144287 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:18:32Z |
| publishDate | 2020 |
| record_format | dspace |
| spelling | ntu-10356/1442872020-10-29T20:11:04Z Autofluorescence lifetime microscopy in normal and cancer cells and nanosecond pulsed electric field effects Awasthi, Kamlesh Change, Feng-Lin Hsu, Hsin-Yun Hsieh, Pei-Ying Ohta, Nobuhiro Asian Spectroscopy Conference 2020 Institute of Advanced Studies Science::Chemistry Fluorescence Lifetime Spectroscopy Autofluorescence Electric field effects on dynamics and function of biological systems depend on the cell membrane capacitance and on the pulse duration of the applied electric field, which enables selective application of pulsed electric field in specific organelles and specific living cells. If electric field whose pulse width is shorter than the charging time of the outer membrane is applied to living systems, the pulsed electric field may penetrate into the cell and induce changes in intracellular function. In fact, we reported the apoptosis induced by application of nanosecond pulsed electric field (nsPEF) with a pulse width of 10–50 ns in HeLa cells.1 Published version 2020-10-27T02:15:57Z 2020-10-27T02:15:57Z 2020 Conference Paper Awasthi, K., Change, F.-L., Hsu, H.-Y., Hsieh, P.-Y., & Ohta, N. (2020). Autofluorescence lifetime microscopy in normal and cancer cells and nanosecond pulsed electric field effects. Proc. Of the 7th Asian Spectroscopy Conference (ASC 2020). doi:10.32655/ASC_8-10_Dec2020.40 https://hdl.handle.net/10356/144287 10.32655/ASC_8-10_Dec2020.40 en © 2020 Nanyang Technological University. application/pdf |
| spellingShingle | Science::Chemistry Fluorescence Lifetime Spectroscopy Autofluorescence Awasthi, Kamlesh Change, Feng-Lin Hsu, Hsin-Yun Hsieh, Pei-Ying Ohta, Nobuhiro Autofluorescence lifetime microscopy in normal and cancer cells and nanosecond pulsed electric field effects |
| title | Autofluorescence lifetime microscopy in normal and cancer cells and nanosecond pulsed electric field effects |
| title_full | Autofluorescence lifetime microscopy in normal and cancer cells and nanosecond pulsed electric field effects |
| title_fullStr | Autofluorescence lifetime microscopy in normal and cancer cells and nanosecond pulsed electric field effects |
| title_full_unstemmed | Autofluorescence lifetime microscopy in normal and cancer cells and nanosecond pulsed electric field effects |
| title_short | Autofluorescence lifetime microscopy in normal and cancer cells and nanosecond pulsed electric field effects |
| title_sort | autofluorescence lifetime microscopy in normal and cancer cells and nanosecond pulsed electric field effects |
| topic | Science::Chemistry Fluorescence Lifetime Spectroscopy Autofluorescence |
| url | https://hdl.handle.net/10356/144287 |
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