A simple strategy to enhance the in vivo wound-healing activity of curcumin in the form of self-assembled nanoparticle complex of curcumin and oligochitosan
While the wound healing activity of curcumin (CUR) has been well-established, its clinical effectiveness remains limited due to the inherently low aqueous CUR solubility, resulting in suboptimal CUR exposure in the wound sites. Previously, we developed high-payload amorphous nanoparticle complex (or...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2020
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| Online Access: | https://hdl.handle.net/10356/144568 |
| _version_ | 1811683536272359424 |
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| author | Nguyen, Minh-Hiep Lee, Suen Ern Tran, The-Thien Bui, Chi-Bao Nguyen, Thi-Huynh-Nga Vu, Ngoc-Bich-Dao Tran, Thi-Thuy Nguyen, Trong-Hoanh-Phong Nguyen, Thi-Thu Hadinoto, Kunn |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Nguyen, Minh-Hiep Lee, Suen Ern Tran, The-Thien Bui, Chi-Bao Nguyen, Thi-Huynh-Nga Vu, Ngoc-Bich-Dao Tran, Thi-Thuy Nguyen, Trong-Hoanh-Phong Nguyen, Thi-Thu Hadinoto, Kunn |
| author_sort | Nguyen, Minh-Hiep |
| collection | NTU |
| description | While the wound healing activity of curcumin (CUR) has been well-established, its clinical effectiveness remains limited due to the inherently low aqueous CUR solubility, resulting in suboptimal CUR exposure in the wound sites. Previously, we developed high-payload amorphous nanoparticle complex (or nanoplex) of CUR and chitosan (CHI) capable of CUR solubility enhancement by drug-polyelectrolyte complexation. The CUR-CHI nanoplex, however, exhibited poor colloidal stability due to its strong agglomeration tendency. Herein we hypothesized that the colloidal stability could be improved by replacing CHI with its oligomers (OCHI) owed to the better charge distribution in OCHI. The effects of key parameters in drug-polyelectrolyte complexation (i.e. pH, salt inclusion, CUR concentration, and OCHI/CUR charge ratio) on the physical characteristics and preparation efficiency of the CUR-OCHI nanoplex produced were investigated. The in vivo wound healing efficacy of the CUR-OCHI nanoplex and its cytotoxicity towards human keratinocytes cells were examined. The results showed that CUR-OCHI nanoplex exhibited prolonged colloidal stability (72 h versus <24 h for the CUR-CHI nanoplex). At the optimal condition, the CUR-OCHI nanoplex (without ultrasonication) exhibited size, zeta potential, and CUR payload of ≈140 nm, 20 mV, and 78% (w/w), respectively. The nanoplex preparation was simple yet robust at nearly 100% CUR utilization rate. The CUR-OCHI nanoplex exhibited superior wound healing efficacy to the native CUR with wound closure of >90% after 7 days versus 9 days for the native CUR resulting in smaller scars, attributed to its generation of high CUR concentration in the wound sites. |
| first_indexed | 2024-10-01T04:14:17Z |
| format | Journal Article |
| id | ntu-10356/144568 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:14:17Z |
| publishDate | 2020 |
| record_format | dspace |
| spelling | ntu-10356/1445682023-12-29T06:47:33Z A simple strategy to enhance the in vivo wound-healing activity of curcumin in the form of self-assembled nanoparticle complex of curcumin and oligochitosan Nguyen, Minh-Hiep Lee, Suen Ern Tran, The-Thien Bui, Chi-Bao Nguyen, Thi-Huynh-Nga Vu, Ngoc-Bich-Dao Tran, Thi-Thuy Nguyen, Trong-Hoanh-Phong Nguyen, Thi-Thu Hadinoto, Kunn School of Chemical and Biomedical Engineering Engineering::Chemical engineering Wound Healing Agent Curcumin While the wound healing activity of curcumin (CUR) has been well-established, its clinical effectiveness remains limited due to the inherently low aqueous CUR solubility, resulting in suboptimal CUR exposure in the wound sites. Previously, we developed high-payload amorphous nanoparticle complex (or nanoplex) of CUR and chitosan (CHI) capable of CUR solubility enhancement by drug-polyelectrolyte complexation. The CUR-CHI nanoplex, however, exhibited poor colloidal stability due to its strong agglomeration tendency. Herein we hypothesized that the colloidal stability could be improved by replacing CHI with its oligomers (OCHI) owed to the better charge distribution in OCHI. The effects of key parameters in drug-polyelectrolyte complexation (i.e. pH, salt inclusion, CUR concentration, and OCHI/CUR charge ratio) on the physical characteristics and preparation efficiency of the CUR-OCHI nanoplex produced were investigated. The in vivo wound healing efficacy of the CUR-OCHI nanoplex and its cytotoxicity towards human keratinocytes cells were examined. The results showed that CUR-OCHI nanoplex exhibited prolonged colloidal stability (72 h versus <24 h for the CUR-CHI nanoplex). At the optimal condition, the CUR-OCHI nanoplex (without ultrasonication) exhibited size, zeta potential, and CUR payload of ≈140 nm, 20 mV, and 78% (w/w), respectively. The nanoplex preparation was simple yet robust at nearly 100% CUR utilization rate. The CUR-OCHI nanoplex exhibited superior wound healing efficacy to the native CUR with wound closure of >90% after 7 days versus 9 days for the native CUR resulting in smaller scars, attributed to its generation of high CUR concentration in the wound sites. Nanyang Technological University Accepted version The authors would like to acknowledge the research funds from Vietnam Atomic Energy Institute and Nuclear Research Institute (Grant number: 07/17/VNCHN) and from Nanyang Technological University's Undergraduate Research Experience on Campus (URECA) for Suen Ern Lee. 2020-11-13T01:49:13Z 2020-11-13T01:49:13Z 2018 Journal Article Nguyen, M.-H., Lee, S. E., Tran, T.-T., Bui, C.-B., Nguyen, T.-H.-N., Vu, N.-B.-D., ... Hadinoto, K. (2019). A simple strategy to enhance the in vivo wound-healing activity of curcumin in the form of self-assembled nanoparticle complex of curcumin and oligochitosan. Materials Science and Engineering: C, 98, 54-64. doi:10.1016/j.msec.2018.12.091 0928-4931 https://hdl.handle.net/10356/144568 10.1016/j.msec.2018.12.091 30813056 98 54 64 en Materials Science and Engineering: C © 2018 Elsevier B.V. All rights reserved. This paper was published in Materials Science and Engineering: C and is made available with permission of Elsevier B.V. application/pdf |
| spellingShingle | Engineering::Chemical engineering Wound Healing Agent Curcumin Nguyen, Minh-Hiep Lee, Suen Ern Tran, The-Thien Bui, Chi-Bao Nguyen, Thi-Huynh-Nga Vu, Ngoc-Bich-Dao Tran, Thi-Thuy Nguyen, Trong-Hoanh-Phong Nguyen, Thi-Thu Hadinoto, Kunn A simple strategy to enhance the in vivo wound-healing activity of curcumin in the form of self-assembled nanoparticle complex of curcumin and oligochitosan |
| title | A simple strategy to enhance the in vivo wound-healing activity of curcumin in the form of self-assembled nanoparticle complex of curcumin and oligochitosan |
| title_full | A simple strategy to enhance the in vivo wound-healing activity of curcumin in the form of self-assembled nanoparticle complex of curcumin and oligochitosan |
| title_fullStr | A simple strategy to enhance the in vivo wound-healing activity of curcumin in the form of self-assembled nanoparticle complex of curcumin and oligochitosan |
| title_full_unstemmed | A simple strategy to enhance the in vivo wound-healing activity of curcumin in the form of self-assembled nanoparticle complex of curcumin and oligochitosan |
| title_short | A simple strategy to enhance the in vivo wound-healing activity of curcumin in the form of self-assembled nanoparticle complex of curcumin and oligochitosan |
| title_sort | simple strategy to enhance the in vivo wound healing activity of curcumin in the form of self assembled nanoparticle complex of curcumin and oligochitosan |
| topic | Engineering::Chemical engineering Wound Healing Agent Curcumin |
| url | https://hdl.handle.net/10356/144568 |
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