Magnetic digital microfluidics on a bioinspired surface for point-of-care diagnostics of infectious disease
Magnetic digital microfluidics uses magnetic force to manipulate droplets on a Teflon-coated substrate through the added magnetic particles. To achieve a wide range of droplet manipulation, hydrophilic patterns, known as surface energy traps, are introduced onto the Teflon-coated hydrophobic substra...
| Main Authors: | , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2020
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/144957 |
| _version_ | 1826111512894242816 |
|---|---|
| author | Kanitthamniyom, Pojchanun Zhang, Yi |
| author2 | School of Mechanical and Aerospace Engineering |
| author_facet | School of Mechanical and Aerospace Engineering Kanitthamniyom, Pojchanun Zhang, Yi |
| author_sort | Kanitthamniyom, Pojchanun |
| collection | NTU |
| description | Magnetic digital microfluidics uses magnetic force to manipulate droplets on a Teflon-coated substrate through the added magnetic particles. To achieve a wide range of droplet manipulation, hydrophilic patterns, known as surface energy traps, are introduced onto the Teflon-coated hydrophobic substrate. However, the Teflon-coated substrate is difficult to modify because it is nonwettable, and existing techniques for patterning surface energy traps have many limitations. Inspired by the mussel adhesion mechanism, we use polydopamine, a bioinspired substance that adheres strongly to almost any materials, to pattern surface energy traps on the Teflon-coated substrate with a great ease. We have optimized the polydopamine coating protocol and characterized the surface properties of the polydopamine surface energy traps. Droplet operations including particle extraction, liquid dispensing, liquid shaping, and cross-platform transfer have been demonstrated on the polydopamine surface energy trap-enabled magnetic digital microfluidic platform in both single-plate and two-plate configurations. Furthermore, the detection of hepatitis B surface antigen using ELISA has been demonstrated on the new magnetic dgitial microfluidic platform. This new bioinspired magnetic digital microfluidic platform is easy to fabricate and operate, showing a great potential for point-of-care applications. |
| first_indexed | 2024-10-01T02:51:58Z |
| format | Journal Article |
| id | ntu-10356/144957 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:51:58Z |
| publishDate | 2020 |
| record_format | dspace |
| spelling | ntu-10356/1449572020-12-07T01:59:43Z Magnetic digital microfluidics on a bioinspired surface for point-of-care diagnostics of infectious disease Kanitthamniyom, Pojchanun Zhang, Yi School of Mechanical and Aerospace Engineering Singapore Centre for 3D Printing Engineering::Mechanical engineering Diagnostics Droplet Magnetic digital microfluidics uses magnetic force to manipulate droplets on a Teflon-coated substrate through the added magnetic particles. To achieve a wide range of droplet manipulation, hydrophilic patterns, known as surface energy traps, are introduced onto the Teflon-coated hydrophobic substrate. However, the Teflon-coated substrate is difficult to modify because it is nonwettable, and existing techniques for patterning surface energy traps have many limitations. Inspired by the mussel adhesion mechanism, we use polydopamine, a bioinspired substance that adheres strongly to almost any materials, to pattern surface energy traps on the Teflon-coated substrate with a great ease. We have optimized the polydopamine coating protocol and characterized the surface properties of the polydopamine surface energy traps. Droplet operations including particle extraction, liquid dispensing, liquid shaping, and cross-platform transfer have been demonstrated on the polydopamine surface energy trap-enabled magnetic digital microfluidic platform in both single-plate and two-plate configurations. Furthermore, the detection of hepatitis B surface antigen using ELISA has been demonstrated on the new magnetic dgitial microfluidic platform. This new bioinspired magnetic digital microfluidic platform is easy to fabricate and operate, showing a great potential for point-of-care applications. Ministry of Education (MOE) Accepted version The authors would like to thank the Start‐Up Grant from the School of Mechanical and Aerospace Engineering at Nanyang Technological University. This work is also supported by Singapore Ministry of Education Tier 1 Grant (RG49/17) and by the Ageing Research Institute for Society and Education (ARISE), Nanyang Technological University, Singapore (ARISE/2017/22). 2020-12-07T01:59:43Z 2020-12-07T01:59:43Z 2019 Journal Article Kanitthamniyom, P., & Zhang, Y. (2019). Magnetic digital microfluidics on a bioinspired surface for point‐of‐care diagnostics of infectious disease. ELECTROPHORESIS, 40(8), 1178–1185. doi:10.1002/elps.201900074 0173-0835 https://hdl.handle.net/10356/144957 10.1002/elps.201900074 30770588 8 40 1178 1185 en Electrophoresis This is the accepted version of the following article: Kanitthamniyom, P., & Zhang, Y. (2019). Magnetic digital microfluidics on a bioinspired surface for point‐of‐care diagnostics of infectious disease. ELECTROPHORESIS, 40(8), 1178–1185., which has been published in final form at doi:10.1002/elps.201900074. This article may be used for non-commercial purposes in accordance with the Wiley Self-Archiving Policy [https://authorservices.wiley.com/authorresources/Journal-Authors/licensing/self-archiving.html]. application/pdf |
| spellingShingle | Engineering::Mechanical engineering Diagnostics Droplet Kanitthamniyom, Pojchanun Zhang, Yi Magnetic digital microfluidics on a bioinspired surface for point-of-care diagnostics of infectious disease |
| title | Magnetic digital microfluidics on a bioinspired surface for point-of-care diagnostics of infectious disease |
| title_full | Magnetic digital microfluidics on a bioinspired surface for point-of-care diagnostics of infectious disease |
| title_fullStr | Magnetic digital microfluidics on a bioinspired surface for point-of-care diagnostics of infectious disease |
| title_full_unstemmed | Magnetic digital microfluidics on a bioinspired surface for point-of-care diagnostics of infectious disease |
| title_short | Magnetic digital microfluidics on a bioinspired surface for point-of-care diagnostics of infectious disease |
| title_sort | magnetic digital microfluidics on a bioinspired surface for point of care diagnostics of infectious disease |
| topic | Engineering::Mechanical engineering Diagnostics Droplet |
| url | https://hdl.handle.net/10356/144957 |
| work_keys_str_mv | AT kanitthamniyompojchanun magneticdigitalmicrofluidicsonabioinspiredsurfaceforpointofcarediagnosticsofinfectiousdisease AT zhangyi magneticdigitalmicrofluidicsonabioinspiredsurfaceforpointofcarediagnosticsofinfectiousdisease |