Modeling the dual oxygen- and pH-stimulated response of hemoglobin-loaded polyampholyte hydrogel for oxygen-pH coupled biosensor platform
This paper presents the dual oxygen- and pH-stimulated responsive performances of hemoglobin-loaded polyampholyte hydrogel, where a multiphysics model is formulated for the numerical characterization of the hydrogel, incorporating electrical interactions of the fixed-mobile charge groups and chemica...
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Format: | Journal Article |
Language: | English |
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2020
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Online Access: | https://hdl.handle.net/10356/144963 |
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author | Goh, Kek Boon Li, Hua Lam, Khin Yong |
author2 | School of Mechanical and Aerospace Engineering |
author_facet | School of Mechanical and Aerospace Engineering Goh, Kek Boon Li, Hua Lam, Khin Yong |
author_sort | Goh, Kek Boon |
collection | NTU |
description | This paper presents the dual oxygen- and pH-stimulated responsive performances of hemoglobin-loaded polyampholyte hydrogel, where a multiphysics model is formulated for the numerical characterization of the hydrogel, incorporating electrical interactions of the fixed-mobile charge groups and chemical reactions of the hemoglobin-oxygen complexes. A developed constitutive relation is integrated into the model to capture bioactivity of the immobilized hemoglobin as a function of ambient oxygen coupled with environmental pH. After examination with published experimental observations, it is concluded that the multiphysics model can accurately characterize both neonatal hemoglobin oxygen saturation and pH-driven volume transition behaviors of hemoglobin-loaded hydrogel. The results show that the swelling deformation of polyampholyte hydrogel changes in a bowl-shaped like pattern with increase of environmental pH value, whereas the rate of oxygen loaded into the hydrogel enlarges linearly with increase of physiological oxygen level from 1 to 30 mmHg. Consequently, these findings demonstrate that hemoglobin-loaded polyampholyte hydrogel could provide an innovative avenue for sensing and storing both bioactive oxygen and hydrogen solutes in biological fluids, pointing to a novel material platform for developing oxygen-pH stimuli coupled responsive biosensor, as well as oxygen- and/or pH-driven bio-actuators. |
first_indexed | 2024-10-01T04:27:09Z |
format | Journal Article |
id | ntu-10356/144963 |
institution | Nanyang Technological University |
language | English |
last_indexed | 2024-10-01T04:27:09Z |
publishDate | 2020 |
record_format | dspace |
spelling | ntu-10356/1449632021-02-08T06:49:54Z Modeling the dual oxygen- and pH-stimulated response of hemoglobin-loaded polyampholyte hydrogel for oxygen-pH coupled biosensor platform Goh, Kek Boon Li, Hua Lam, Khin Yong School of Mechanical and Aerospace Engineering Engineering::Mechanical engineering Dual Oxygen-pH-sensitive Hydrogel Hemoglobin-loaded Polyampholyte Hydrogel This paper presents the dual oxygen- and pH-stimulated responsive performances of hemoglobin-loaded polyampholyte hydrogel, where a multiphysics model is formulated for the numerical characterization of the hydrogel, incorporating electrical interactions of the fixed-mobile charge groups and chemical reactions of the hemoglobin-oxygen complexes. A developed constitutive relation is integrated into the model to capture bioactivity of the immobilized hemoglobin as a function of ambient oxygen coupled with environmental pH. After examination with published experimental observations, it is concluded that the multiphysics model can accurately characterize both neonatal hemoglobin oxygen saturation and pH-driven volume transition behaviors of hemoglobin-loaded hydrogel. The results show that the swelling deformation of polyampholyte hydrogel changes in a bowl-shaped like pattern with increase of environmental pH value, whereas the rate of oxygen loaded into the hydrogel enlarges linearly with increase of physiological oxygen level from 1 to 30 mmHg. Consequently, these findings demonstrate that hemoglobin-loaded polyampholyte hydrogel could provide an innovative avenue for sensing and storing both bioactive oxygen and hydrogen solutes in biological fluids, pointing to a novel material platform for developing oxygen-pH stimuli coupled responsive biosensor, as well as oxygen- and/or pH-driven bio-actuators. Accepted version 2020-12-07T03:15:38Z 2020-12-07T03:15:38Z 2019 Journal Article Goh, K. B., Li, H., & Lam, K. Y. (2019). Modeling the dual oxygen- and pH-stimulated response of hemoglobin-loaded polyampholyte hydrogel for oxygen-pH coupled biosensor platform. Sensors and Actuators B: Chemical, 286, 421–428. doi:10.1016/j.snb.2019.01.138 0925-4005 https://hdl.handle.net/10356/144963 10.1016/j.snb.2019.01.138 286 421 428 en Sensors and Actuators B: Chemical © 2019 Elsevier B.V. All rights reserved. This paper was published in Sensors and Actuators B: Chemical and is made available with permission of Elsevier B.V. application/pdf |
spellingShingle | Engineering::Mechanical engineering Dual Oxygen-pH-sensitive Hydrogel Hemoglobin-loaded Polyampholyte Hydrogel Goh, Kek Boon Li, Hua Lam, Khin Yong Modeling the dual oxygen- and pH-stimulated response of hemoglobin-loaded polyampholyte hydrogel for oxygen-pH coupled biosensor platform |
title | Modeling the dual oxygen- and pH-stimulated response of hemoglobin-loaded polyampholyte hydrogel for oxygen-pH coupled biosensor platform |
title_full | Modeling the dual oxygen- and pH-stimulated response of hemoglobin-loaded polyampholyte hydrogel for oxygen-pH coupled biosensor platform |
title_fullStr | Modeling the dual oxygen- and pH-stimulated response of hemoglobin-loaded polyampholyte hydrogel for oxygen-pH coupled biosensor platform |
title_full_unstemmed | Modeling the dual oxygen- and pH-stimulated response of hemoglobin-loaded polyampholyte hydrogel for oxygen-pH coupled biosensor platform |
title_short | Modeling the dual oxygen- and pH-stimulated response of hemoglobin-loaded polyampholyte hydrogel for oxygen-pH coupled biosensor platform |
title_sort | modeling the dual oxygen and ph stimulated response of hemoglobin loaded polyampholyte hydrogel for oxygen ph coupled biosensor platform |
topic | Engineering::Mechanical engineering Dual Oxygen-pH-sensitive Hydrogel Hemoglobin-loaded Polyampholyte Hydrogel |
url | https://hdl.handle.net/10356/144963 |
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