Heterogeneous somatostatin-expressing neuron population in mouse ventral tegmental area
The cellular architecture of the ventral tegmental area (VTA), the main hub of the brain reward system, remains only partially characterized. To extend the characterization to inhibitory neurons, we have identified three distinct subtypes of somatostatin (Sst)-expressing neurons in the mouse VTA. Th...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2020
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| Online Access: | https://hdl.handle.net/10356/145372 |
| _version_ | 1824455734713122816 |
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| author | Nagaeva, Elina Zubarev, Ivan Gonzales, Carolina Bengtsson Forss, Mikko Nikouei, Kasra de Miguel, Elena Elsilä, Lauri Linden, Anni-Maija Hjerling-Leffler, Jens Augustine, George James Korpi, Esa R. |
| author2 | Lee Kong Chian School of Medicine (LKCMedicine) |
| author_facet | Lee Kong Chian School of Medicine (LKCMedicine) Nagaeva, Elina Zubarev, Ivan Gonzales, Carolina Bengtsson Forss, Mikko Nikouei, Kasra de Miguel, Elena Elsilä, Lauri Linden, Anni-Maija Hjerling-Leffler, Jens Augustine, George James Korpi, Esa R. |
| author_sort | Nagaeva, Elina |
| collection | NTU |
| description | The cellular architecture of the ventral tegmental area (VTA), the main hub of the brain reward system, remains only partially characterized. To extend the characterization to inhibitory neurons, we have identified three distinct subtypes of somatostatin (Sst)-expressing neurons in the mouse VTA. These neurons differ in their electrophysiological and morphological properties, anatomical localization, as well as mRNA expression profiles. Importantly, similar to cortical Sst-containing interneurons, most VTA Sst neurons express GABAergic inhibitory markers, but some of them also express glutamatergic excitatory markers and a subpopulation even express dopaminergic markers. Furthermore, only some of the proposed marker genes for cortical Sst neurons were expressed in the VTA Sst neurons. Physiologically, one of the VTA Sst neuron subtypes locally inhibited neighboring dopamine neurons. Overall, our results demonstrate the remarkable complexity and heterogeneity of VTA Sst neurons and suggest that these cells are multifunctional players in the midbrain reward circuitry. |
| first_indexed | 2025-02-19T03:42:55Z |
| format | Journal Article |
| id | ntu-10356/145372 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:42:55Z |
| publishDate | 2020 |
| record_format | dspace |
| spelling | ntu-10356/1453722023-03-05T16:43:37Z Heterogeneous somatostatin-expressing neuron population in mouse ventral tegmental area Nagaeva, Elina Zubarev, Ivan Gonzales, Carolina Bengtsson Forss, Mikko Nikouei, Kasra de Miguel, Elena Elsilä, Lauri Linden, Anni-Maija Hjerling-Leffler, Jens Augustine, George James Korpi, Esa R. Lee Kong Chian School of Medicine (LKCMedicine) Science::Medicine Neuron Midbrain The cellular architecture of the ventral tegmental area (VTA), the main hub of the brain reward system, remains only partially characterized. To extend the characterization to inhibitory neurons, we have identified three distinct subtypes of somatostatin (Sst)-expressing neurons in the mouse VTA. These neurons differ in their electrophysiological and morphological properties, anatomical localization, as well as mRNA expression profiles. Importantly, similar to cortical Sst-containing interneurons, most VTA Sst neurons express GABAergic inhibitory markers, but some of them also express glutamatergic excitatory markers and a subpopulation even express dopaminergic markers. Furthermore, only some of the proposed marker genes for cortical Sst neurons were expressed in the VTA Sst neurons. Physiologically, one of the VTA Sst neuron subtypes locally inhibited neighboring dopamine neurons. Overall, our results demonstrate the remarkable complexity and heterogeneity of VTA Sst neurons and suggest that these cells are multifunctional players in the midbrain reward circuitry. Ministry of Education (MOE) Published version The project was funded by the Academy of Finland (1278174 and 1317399), The Finnish National Agency for Education EDUFI, the Sigrid Juselius Foundation, and research grants MOE2015-T2-2-095 and MOE2017-T3-1-002 from the Singapore Ministry of Education. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. 2020-12-18T08:26:52Z 2020-12-18T08:26:52Z 2020 Journal Article Nagaeva, E., Zubarev, I., Gonzales, C. B., Forss, M., Nikouei, K., de Miguel, E., . . . Korpi, E. R. (2020). Heterogeneous somatostatin-expressing neuron population in mouse ventral tegmental area. eLife, 9, 1-29. doi:10.7554/eLife.59328 2050-084X https://hdl.handle.net/10356/145372 10.7554/eLife.59328 32749220 9 1 29 en MOE2015-T2-2-095 MOE2017-T3-1-002 eLife © 2020 Nagaeva et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. application/pdf |
| spellingShingle | Science::Medicine Neuron Midbrain Nagaeva, Elina Zubarev, Ivan Gonzales, Carolina Bengtsson Forss, Mikko Nikouei, Kasra de Miguel, Elena Elsilä, Lauri Linden, Anni-Maija Hjerling-Leffler, Jens Augustine, George James Korpi, Esa R. Heterogeneous somatostatin-expressing neuron population in mouse ventral tegmental area |
| title | Heterogeneous somatostatin-expressing neuron population in mouse ventral tegmental area |
| title_full | Heterogeneous somatostatin-expressing neuron population in mouse ventral tegmental area |
| title_fullStr | Heterogeneous somatostatin-expressing neuron population in mouse ventral tegmental area |
| title_full_unstemmed | Heterogeneous somatostatin-expressing neuron population in mouse ventral tegmental area |
| title_short | Heterogeneous somatostatin-expressing neuron population in mouse ventral tegmental area |
| title_sort | heterogeneous somatostatin expressing neuron population in mouse ventral tegmental area |
| topic | Science::Medicine Neuron Midbrain |
| url | https://hdl.handle.net/10356/145372 |
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