Far-field unlabeled super-resolution imaging with superoscillatory illumination
Unlabeled super-resolution is the next grand challenge in imaging. Stimulated emission depletion and single-molecule microscopies have revolutionized the life sciences but are still limited by the need for reporters (labels) embedded within the sample. While the Veselago–Pendry “super-lens,” using a...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2020
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| Online Access: | https://hdl.handle.net/10356/145405 |
| _version_ | 1811687403662868480 |
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| author | Rogers, Edward T. F. Quraishe, Shmma Rogers, Katrine S. Newman, Tracey A. Smith, Peter J. S. Zheludev, Nikolay I. |
| author2 | School of Physical and Mathematical Sciences |
| author_facet | School of Physical and Mathematical Sciences Rogers, Edward T. F. Quraishe, Shmma Rogers, Katrine S. Newman, Tracey A. Smith, Peter J. S. Zheludev, Nikolay I. |
| author_sort | Rogers, Edward T. F. |
| collection | NTU |
| description | Unlabeled super-resolution is the next grand challenge in imaging. Stimulated emission depletion and single-molecule microscopies have revolutionized the life sciences but are still limited by the need for reporters (labels) embedded within the sample. While the Veselago–Pendry “super-lens,” using a negative-index metamaterial, is a promising idea for imaging beyond the diffraction limit, there are substantial technological challenges to its realization. Another route to far-field subwavelength focusing is using optical superoscillations: engineered interference of multiple coherent waves creating an, in principle, arbitrarily small hotspot. Here, we demonstrate microscopy with superoscillatory illumination of the object and describe its underlying principles. We show that far-field images taken with superoscillatory illumination are themselves superoscillatory and, hence, can reveal fine structural details of the object that are lost in conventional far-field imaging. We show that the resolution of a superoscillatory microscope is determined by the size of the hotspot, rather than the bandwidth of the optical instrument. We demonstrate high-frame-rate polarization-contrast imaging of unmodified living cells with a resolution significantly exceeding that achievable with conventional instruments. This non-algorithmic, low-phototoxicity imaging technology is a powerful tool both for biological research and for super-resolution imaging of samples that do not allow labeling, such as the interior of silicon chips. |
| first_indexed | 2024-10-01T05:15:46Z |
| format | Journal Article |
| id | ntu-10356/145405 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:15:46Z |
| publishDate | 2020 |
| record_format | dspace |
| spelling | ntu-10356/1454052023-02-28T19:26:12Z Far-field unlabeled super-resolution imaging with superoscillatory illumination Rogers, Edward T. F. Quraishe, Shmma Rogers, Katrine S. Newman, Tracey A. Smith, Peter J. S. Zheludev, Nikolay I. School of Physical and Mathematical Sciences Centre for Disruptive Photonic Technologies (CDPT) The Photonics Institute Science::Physics Bioinformatics Diffraction Unlabeled super-resolution is the next grand challenge in imaging. Stimulated emission depletion and single-molecule microscopies have revolutionized the life sciences but are still limited by the need for reporters (labels) embedded within the sample. While the Veselago–Pendry “super-lens,” using a negative-index metamaterial, is a promising idea for imaging beyond the diffraction limit, there are substantial technological challenges to its realization. Another route to far-field subwavelength focusing is using optical superoscillations: engineered interference of multiple coherent waves creating an, in principle, arbitrarily small hotspot. Here, we demonstrate microscopy with superoscillatory illumination of the object and describe its underlying principles. We show that far-field images taken with superoscillatory illumination are themselves superoscillatory and, hence, can reveal fine structural details of the object that are lost in conventional far-field imaging. We show that the resolution of a superoscillatory microscope is determined by the size of the hotspot, rather than the bandwidth of the optical instrument. We demonstrate high-frame-rate polarization-contrast imaging of unmodified living cells with a resolution significantly exceeding that achievable with conventional instruments. This non-algorithmic, low-phototoxicity imaging technology is a powerful tool both for biological research and for super-resolution imaging of samples that do not allow labeling, such as the interior of silicon chips. Ministry of Education (MOE) Published version This research was supported by Wessex Medical Research (Grant No. WMR03), the University of Southampton: Institute for Life Sciences and Enterprise Fund, the UK’s Engineering and Physical Sciences Research Council (Grant No. EP/M009122/1), and the Singapore Ministry of Education [Grant No. MOE2016-T3-1-006 (S)]. The authors would like to thank Guanghui Yuan for numerous fruitful discussions; Alexander Buchnev and Jun Yu Ou for fabrication of the test masks; Grace Hallinan, Aleks Pitera, and Katrin Deinhardt for assistance with the neuronal cultures; Rudolf Oldenbourg for fruitful discussions; and Mark Willet of the Microscopy Facility in Biological Sciences at the University of Southampton for the matched fluorescent and DIC photos of HeLa cells used in Fig. 3. The authors declare no competing financial interests. 2020-12-21T04:14:01Z 2020-12-21T04:14:01Z 2020 Journal Article Rogers, E. T. F., Quraishe, S., Rogers, K. S., Newman, T. A., Smith, P. J. S., & Zheludev, N. I. (2020). Far-field unlabeled super-resolution imaging with superoscillatory illumination. APL Photonics, 5(6), 066107-. doi:10.1063/1.5144918 2378-0967 https://hdl.handle.net/10356/145405 10.1063/1.5144918 6 5 en MOE2016-T3-1-006 (S) APL Photonics © 2020 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license(http://creativecommons.org/licenses/by/4.0/). application/pdf |
| spellingShingle | Science::Physics Bioinformatics Diffraction Rogers, Edward T. F. Quraishe, Shmma Rogers, Katrine S. Newman, Tracey A. Smith, Peter J. S. Zheludev, Nikolay I. Far-field unlabeled super-resolution imaging with superoscillatory illumination |
| title | Far-field unlabeled super-resolution imaging with superoscillatory illumination |
| title_full | Far-field unlabeled super-resolution imaging with superoscillatory illumination |
| title_fullStr | Far-field unlabeled super-resolution imaging with superoscillatory illumination |
| title_full_unstemmed | Far-field unlabeled super-resolution imaging with superoscillatory illumination |
| title_short | Far-field unlabeled super-resolution imaging with superoscillatory illumination |
| title_sort | far field unlabeled super resolution imaging with superoscillatory illumination |
| topic | Science::Physics Bioinformatics Diffraction |
| url | https://hdl.handle.net/10356/145405 |
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