Characterisation of CaCO3 phases during strain-specific ureolytic precipitation
Numerous microbial species can selectively precipitate mineral carbonates with enhanced mechanical properties, however, understanding exactly how they achieve this control represents a major challenge in the field of biomineralisation. We have studied microbial induced calcium carbonate (CaCO3) prec...
| Main Authors: | , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2021
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| Online Access: | https://hdl.handle.net/10356/146056 |
| _version_ | 1811693661970235392 |
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| author | Clarà Saracho, Alexandra Haigh, Stuart K. Hata, Toshiro Soga, Kenichi Farsang, Stefan Redfern, Simon Anthony Turner Marek, Ewa |
| author2 | Asian School of the Environment |
| author_facet | Asian School of the Environment Clarà Saracho, Alexandra Haigh, Stuart K. Hata, Toshiro Soga, Kenichi Farsang, Stefan Redfern, Simon Anthony Turner Marek, Ewa |
| author_sort | Clarà Saracho, Alexandra |
| collection | NTU |
| description | Numerous microbial species can selectively precipitate mineral carbonates with enhanced mechanical properties, however, understanding exactly how they achieve this control represents a major challenge in the field of biomineralisation. We have studied microbial induced calcium carbonate (CaCO3) precipitation (MICP) in three ureolytic bacterial strains from the Sporosarcina family, including S. newyorkensis, a newly isolated microbe from the deep sea. We find that the interplay between structural water and strain-specific amino acid groups is fundamental to the stabilisation of vaterite and that, under the same conditions, different isolates yield distinctly different polymorphs. The latter is found to be associated with different urease activities and, consequently, precipitation kinetics, which change depending on pressure-temperature conditions. Further, CaCO3 polymorph selection also depends on the coupled effect of chemical treatment and initial bacterial concentrations. Our findings provide new insights into strain-specific CaCO3 polymorphic selection and stabilisation, and open up promising avenues for designing bio-reinforced geo-materials that capitalise on the different particle bond mechanical properties offered by different polymorphs. |
| first_indexed | 2024-10-01T06:55:14Z |
| format | Journal Article |
| id | ntu-10356/146056 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:55:14Z |
| publishDate | 2021 |
| record_format | dspace |
| spelling | ntu-10356/1460562023-02-28T16:41:41Z Characterisation of CaCO3 phases during strain-specific ureolytic precipitation Clarà Saracho, Alexandra Haigh, Stuart K. Hata, Toshiro Soga, Kenichi Farsang, Stefan Redfern, Simon Anthony Turner Marek, Ewa Asian School of the Environment Engineering::Civil engineering Bioinspired Materials Biomineralization Numerous microbial species can selectively precipitate mineral carbonates with enhanced mechanical properties, however, understanding exactly how they achieve this control represents a major challenge in the field of biomineralisation. We have studied microbial induced calcium carbonate (CaCO3) precipitation (MICP) in three ureolytic bacterial strains from the Sporosarcina family, including S. newyorkensis, a newly isolated microbe from the deep sea. We find that the interplay between structural water and strain-specific amino acid groups is fundamental to the stabilisation of vaterite and that, under the same conditions, different isolates yield distinctly different polymorphs. The latter is found to be associated with different urease activities and, consequently, precipitation kinetics, which change depending on pressure-temperature conditions. Further, CaCO3 polymorph selection also depends on the coupled effect of chemical treatment and initial bacterial concentrations. Our findings provide new insights into strain-specific CaCO3 polymorphic selection and stabilisation, and open up promising avenues for designing bio-reinforced geo-materials that capitalise on the different particle bond mechanical properties offered by different polymorphs. Published version 2021-01-22T04:08:42Z 2021-01-22T04:08:42Z 2020 Journal Article Clarà Saracho, A., Haigh, S. K., Hata, T., Soga, K., Farsang, S., Redfern, S. A. T., & Marek, E. (2020). Characterisation of CaCO3 phases during strain-specific ureolytic precipitation. Scientific Reports, 10(1), 10168-. doi:10.1038/s41598-020-66831-y 2045-2322 https://hdl.handle.net/10356/146056 10.1038/s41598-020-66831-y 32576861 2-s2.0-85086790087 1 10 en Scientific Reports © 2020 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. application/pdf |
| spellingShingle | Engineering::Civil engineering Bioinspired Materials Biomineralization Clarà Saracho, Alexandra Haigh, Stuart K. Hata, Toshiro Soga, Kenichi Farsang, Stefan Redfern, Simon Anthony Turner Marek, Ewa Characterisation of CaCO3 phases during strain-specific ureolytic precipitation |
| title | Characterisation of CaCO3 phases during strain-specific ureolytic precipitation |
| title_full | Characterisation of CaCO3 phases during strain-specific ureolytic precipitation |
| title_fullStr | Characterisation of CaCO3 phases during strain-specific ureolytic precipitation |
| title_full_unstemmed | Characterisation of CaCO3 phases during strain-specific ureolytic precipitation |
| title_short | Characterisation of CaCO3 phases during strain-specific ureolytic precipitation |
| title_sort | characterisation of caco3 phases during strain specific ureolytic precipitation |
| topic | Engineering::Civil engineering Bioinspired Materials Biomineralization |
| url | https://hdl.handle.net/10356/146056 |
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