The novel E. coli cell division protein, YtfB, plays a role in eukaryotic cell adhesion
Characterisation of protein function based solely on homology searches may overlook functions under specific environmental conditions, or the possibility of a protein having multiple roles. In this study we investigated the role of YtfB, a protein originally identified in a genome-wide screen to cau...
| Main Authors: | , , , , , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2021
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/146112 |
| _version_ | 1811686629233917952 |
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| author | Bottomley, Amy L. Peterson, Elizabeth Iosifidis, Gregory Yong, Adeline Mei Hui Hartley-Tassell, Lauren E. Ansari, Shirin McKenzie, Chris Burke, Catherine Duggin, Iain G. Kline, Kimberly A. Harry, Elizabeth J. |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Bottomley, Amy L. Peterson, Elizabeth Iosifidis, Gregory Yong, Adeline Mei Hui Hartley-Tassell, Lauren E. Ansari, Shirin McKenzie, Chris Burke, Catherine Duggin, Iain G. Kline, Kimberly A. Harry, Elizabeth J. |
| author_sort | Bottomley, Amy L. |
| collection | NTU |
| description | Characterisation of protein function based solely on homology searches may overlook functions under specific environmental conditions, or the possibility of a protein having multiple roles. In this study we investigated the role of YtfB, a protein originally identified in a genome-wide screen to cause inhibition of cell division, and has demonstrated to localise to the Escherichia coli division site with some degree of glycan specificity. Interestingly, YtfB also shows homology to the virulence factor OapA from Haemophilus influenzae, which is important for adherence to epithelial cells, indicating the potential of additional function(s) for YtfB. Here we show that E. coli YtfB binds to N’acetylglucosamine and mannobiose glycans with high affinity. The loss of ytfB results in a reduction in the ability of the uropathogenic E. coli strain UTI89 to adhere to human kidney cells, but not to bladder cells, suggesting a specific role in the initial adherence stage of ascending urinary tract infections. Taken together, our results suggest a role for YtfB in adhesion to specific eukaryotic cells, which may be additional, or complementary, to its role in cell division. This study highlights the importance of understanding the possible multiple functions of proteins based on homology, which may be specific to different environmental conditions. |
| first_indexed | 2024-10-01T05:03:27Z |
| format | Journal Article |
| id | ntu-10356/146112 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:03:27Z |
| publishDate | 2021 |
| record_format | dspace |
| spelling | ntu-10356/1461122023-02-28T17:08:34Z The novel E. coli cell division protein, YtfB, plays a role in eukaryotic cell adhesion Bottomley, Amy L. Peterson, Elizabeth Iosifidis, Gregory Yong, Adeline Mei Hui Hartley-Tassell, Lauren E. Ansari, Shirin McKenzie, Chris Burke, Catherine Duggin, Iain G. Kline, Kimberly A. Harry, Elizabeth J. School of Biological Sciences Singapore Centre for Environmental Life Sciences and Engineering (SCELSE) Science::Biological sciences Bacterial Adhesion Bacteriology Characterisation of protein function based solely on homology searches may overlook functions under specific environmental conditions, or the possibility of a protein having multiple roles. In this study we investigated the role of YtfB, a protein originally identified in a genome-wide screen to cause inhibition of cell division, and has demonstrated to localise to the Escherichia coli division site with some degree of glycan specificity. Interestingly, YtfB also shows homology to the virulence factor OapA from Haemophilus influenzae, which is important for adherence to epithelial cells, indicating the potential of additional function(s) for YtfB. Here we show that E. coli YtfB binds to N’acetylglucosamine and mannobiose glycans with high affinity. The loss of ytfB results in a reduction in the ability of the uropathogenic E. coli strain UTI89 to adhere to human kidney cells, but not to bladder cells, suggesting a specific role in the initial adherence stage of ascending urinary tract infections. Taken together, our results suggest a role for YtfB in adhesion to specific eukaryotic cells, which may be additional, or complementary, to its role in cell division. This study highlights the importance of understanding the possible multiple functions of proteins based on homology, which may be specific to different environmental conditions. Published version 2021-01-27T01:14:31Z 2021-01-27T01:14:31Z 2020 Journal Article Bottomley, A. L., Peterson, E., Iosifidis, G., Yong, A. M. H., Hartley-Tassell, L. E., Ansari, S., . . . Harry, E. J. (2020). The novel E. coli cell division protein, YtfB, plays a role in eukaryotic cell adhesion. Scientific Reports, 10(1), 6745-. doi:10.1038/s41598-020-63729-7 2045-2322 0000-0003-4868-7350 0000-0002-5472-3074 0000-0003-0858-9473 https://hdl.handle.net/10356/146112 10.1038/s41598-020-63729-7 32317661 2-s2.0-85083782388 1 10 en Scientific Reports © 2020 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. application/pdf |
| spellingShingle | Science::Biological sciences Bacterial Adhesion Bacteriology Bottomley, Amy L. Peterson, Elizabeth Iosifidis, Gregory Yong, Adeline Mei Hui Hartley-Tassell, Lauren E. Ansari, Shirin McKenzie, Chris Burke, Catherine Duggin, Iain G. Kline, Kimberly A. Harry, Elizabeth J. The novel E. coli cell division protein, YtfB, plays a role in eukaryotic cell adhesion |
| title | The novel E. coli cell division protein, YtfB, plays a role in eukaryotic cell adhesion |
| title_full | The novel E. coli cell division protein, YtfB, plays a role in eukaryotic cell adhesion |
| title_fullStr | The novel E. coli cell division protein, YtfB, plays a role in eukaryotic cell adhesion |
| title_full_unstemmed | The novel E. coli cell division protein, YtfB, plays a role in eukaryotic cell adhesion |
| title_short | The novel E. coli cell division protein, YtfB, plays a role in eukaryotic cell adhesion |
| title_sort | novel e coli cell division protein ytfb plays a role in eukaryotic cell adhesion |
| topic | Science::Biological sciences Bacterial Adhesion Bacteriology |
| url | https://hdl.handle.net/10356/146112 |
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