Genomic, metabolic and phenotypic variability shapes ecological differentiation and intraspecies interactions of Alteromonas macleodii
Ecological differentiation between strains of bacterial species is shaped by genomic and metabolic variability. However, connecting genotypes to ecological niches remains a major challenge. Here, we linked bacterial geno- and phenotypes by contextualizing pangenomic, exometabolomic and physiological...
| Main Authors: | , , , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2021
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| Online Access: | https://hdl.handle.net/10356/146213 |
| _version_ | 1826126675262308352 |
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| author | Koch, Hanna Germscheid, Nora Freese, Heike M. Noriega-Ortega, Beatriz Lücking, Dominik Berger, Martine Qiu, Galaxy Marzinelli, Ezequiel Miguel Campbell, Alexandra H. Steinberg, Peter David Overmann, Jörg Dittmar, Thorsten Simon, Meinhard Wietz, Matthias |
| author2 | Singapore Centre for Environmental Life Sciences and Engineering |
| author_facet | Singapore Centre for Environmental Life Sciences and Engineering Koch, Hanna Germscheid, Nora Freese, Heike M. Noriega-Ortega, Beatriz Lücking, Dominik Berger, Martine Qiu, Galaxy Marzinelli, Ezequiel Miguel Campbell, Alexandra H. Steinberg, Peter David Overmann, Jörg Dittmar, Thorsten Simon, Meinhard Wietz, Matthias |
| author_sort | Koch, Hanna |
| collection | NTU |
| description | Ecological differentiation between strains of bacterial species is shaped by genomic and metabolic variability. However, connecting genotypes to ecological niches remains a major challenge. Here, we linked bacterial geno- and phenotypes by contextualizing pangenomic, exometabolomic and physiological evidence in twelve strains of the marine bacterium Alteromonas macleodii, illuminating adaptive strategies of carbon metabolism, microbial interactions, cellular communication and iron acquisition. In A. macleodii strain MIT1002, secretion of amino acids and the unique capacity for phenol degradation may promote associations with Prochlorococcus cyanobacteria. Strain 83-1 and three novel Pacific isolates, featuring clonal genomes despite originating from distant locations, have profound abilities for algal polysaccharide utilization but without detrimental implications for Ecklonia macroalgae. Degradation of toluene and xylene, mediated via a plasmid syntenic to terrestrial Pseudomonas, was unique to strain EZ55. Benzoate degradation by strain EC673 related to a chromosomal gene cluster shared with the plasmid of A. mediterranea EC615, underlining that mobile genetic elements drive adaptations. Furthermore, we revealed strain-specific production of siderophores and homoserine lactones, with implications for nutrient acquisition and cellular communication. Phenotypic variability corresponded to different competitiveness in co-culture and geographic distribution, indicating linkages between intraspecific diversity, microbial interactions and biogeography. The finding of “ecological microdiversity” helps understanding the widespread occurrence of A. macleodii and contributes to the interpretation of bacterial niche specialization, population ecology and biogeochemical roles. |
| first_indexed | 2024-10-01T06:56:33Z |
| format | Journal Article |
| id | ntu-10356/146213 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:56:33Z |
| publishDate | 2021 |
| record_format | dspace |
| spelling | ntu-10356/1462132021-02-06T20:11:31Z Genomic, metabolic and phenotypic variability shapes ecological differentiation and intraspecies interactions of Alteromonas macleodii Koch, Hanna Germscheid, Nora Freese, Heike M. Noriega-Ortega, Beatriz Lücking, Dominik Berger, Martine Qiu, Galaxy Marzinelli, Ezequiel Miguel Campbell, Alexandra H. Steinberg, Peter David Overmann, Jörg Dittmar, Thorsten Simon, Meinhard Wietz, Matthias Singapore Centre for Environmental Life Sciences and Engineering Science::Biological sciences Marine Microbiology Water Microbiology Ecological differentiation between strains of bacterial species is shaped by genomic and metabolic variability. However, connecting genotypes to ecological niches remains a major challenge. Here, we linked bacterial geno- and phenotypes by contextualizing pangenomic, exometabolomic and physiological evidence in twelve strains of the marine bacterium Alteromonas macleodii, illuminating adaptive strategies of carbon metabolism, microbial interactions, cellular communication and iron acquisition. In A. macleodii strain MIT1002, secretion of amino acids and the unique capacity for phenol degradation may promote associations with Prochlorococcus cyanobacteria. Strain 83-1 and three novel Pacific isolates, featuring clonal genomes despite originating from distant locations, have profound abilities for algal polysaccharide utilization but without detrimental implications for Ecklonia macroalgae. Degradation of toluene and xylene, mediated via a plasmid syntenic to terrestrial Pseudomonas, was unique to strain EZ55. Benzoate degradation by strain EC673 related to a chromosomal gene cluster shared with the plasmid of A. mediterranea EC615, underlining that mobile genetic elements drive adaptations. Furthermore, we revealed strain-specific production of siderophores and homoserine lactones, with implications for nutrient acquisition and cellular communication. Phenotypic variability corresponded to different competitiveness in co-culture and geographic distribution, indicating linkages between intraspecific diversity, microbial interactions and biogeography. The finding of “ecological microdiversity” helps understanding the widespread occurrence of A. macleodii and contributes to the interpretation of bacterial niche specialization, population ecology and biogeochemical roles. Published version 2021-02-02T06:21:22Z 2021-02-02T06:21:22Z 2020 Journal Article Koch, H., Germscheid, N., Freese, H. M., Noriega-Ortega, B., Lücking, D., Berger, M., . . . Wietz, M. (2020). Genomic, metabolic and phenotypic variability shapes ecological differentiation and intraspecies interactions of Alteromonas macleodii. Scientific Reports, 10(1), 809-. doi:10.1038/s41598-020-57526-5 2045-2322 0000-0002-3762-3389 0000-0002-6151-6989 https://hdl.handle.net/10356/146213 10.1038/s41598-020-57526-5 31964928 2-s2.0-85078157306 1 10 en Scientific Reports © 2020 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. application/pdf |
| spellingShingle | Science::Biological sciences Marine Microbiology Water Microbiology Koch, Hanna Germscheid, Nora Freese, Heike M. Noriega-Ortega, Beatriz Lücking, Dominik Berger, Martine Qiu, Galaxy Marzinelli, Ezequiel Miguel Campbell, Alexandra H. Steinberg, Peter David Overmann, Jörg Dittmar, Thorsten Simon, Meinhard Wietz, Matthias Genomic, metabolic and phenotypic variability shapes ecological differentiation and intraspecies interactions of Alteromonas macleodii |
| title | Genomic, metabolic and phenotypic variability shapes ecological differentiation and intraspecies interactions of Alteromonas macleodii |
| title_full | Genomic, metabolic and phenotypic variability shapes ecological differentiation and intraspecies interactions of Alteromonas macleodii |
| title_fullStr | Genomic, metabolic and phenotypic variability shapes ecological differentiation and intraspecies interactions of Alteromonas macleodii |
| title_full_unstemmed | Genomic, metabolic and phenotypic variability shapes ecological differentiation and intraspecies interactions of Alteromonas macleodii |
| title_short | Genomic, metabolic and phenotypic variability shapes ecological differentiation and intraspecies interactions of Alteromonas macleodii |
| title_sort | genomic metabolic and phenotypic variability shapes ecological differentiation and intraspecies interactions of alteromonas macleodii |
| topic | Science::Biological sciences Marine Microbiology Water Microbiology |
| url | https://hdl.handle.net/10356/146213 |
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