Transcriptome profiles of Quercus rubra responding to increased O3 stress
Background: Climate plays an essential role in forest health, and climate change may increase forest productivity losses due to abiotic and biotic stress. Increased temperature leads to the increased formation of ozone (O3). Ozone is formed by the interaction of sunlight, molecular oxygen and by the...
| Главные авторы: | , , , , , , , , , , |
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| Другие авторы: | |
| Формат: | Journal Article |
| Язык: | English |
| Опубликовано: |
2021
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| Предметы: | |
| Online-ссылка: | https://hdl.handle.net/10356/146317 |
| _version_ | 1826128812246564864 |
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| author | Soltani, Nourolah Best, Teo Grace, Dantria Nelms, Christen Shumaker, Ketia Romero-Severson, Jeanne Drautz-Moses, Daniela Isabel Schuster, Stephan Christoph Staton, Margaret Carlson, John Gwinn, Kimberly |
| author2 | Singapore Centre for Environmental Life Sciences and Engineering |
| author_facet | Singapore Centre for Environmental Life Sciences and Engineering Soltani, Nourolah Best, Teo Grace, Dantria Nelms, Christen Shumaker, Ketia Romero-Severson, Jeanne Drautz-Moses, Daniela Isabel Schuster, Stephan Christoph Staton, Margaret Carlson, John Gwinn, Kimberly |
| author_sort | Soltani, Nourolah |
| collection | NTU |
| description | Background: Climate plays an essential role in forest health, and climate change may increase forest productivity losses due to abiotic and biotic stress. Increased temperature leads to the increased formation of ozone (O3). Ozone is formed by the interaction of sunlight, molecular oxygen and by the reactions of chemicals commonly found in industrial and automobile emissions such as nitrogen oxides and volatile organic compounds. Although it is well known that productivity of Northern red oak (Quercus rubra) (NRO), an ecologically and economically important species in the forests of eastern North America, is reduced by exposure to O3, limited information is available on its responses to exogenous stimuli at the level of gene expression. Results: RNA sequencing yielded more than 323 million high-quality raw sequence reads. De novo assembly generated 52,662 unigenes, of which more than 42,000 sequences could be annotated through homology-based searches. A total of 4140 differential expressed genes (DEGs) were detected in response to O3 stress, as compared to their respective controls. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses of the O3-response DEGs revealed perturbation of several biological pathways including energy, lipid, amino acid, carbohydrate and terpenoid metabolism as well as plant-pathogen interaction. Conclusion: This study provides the first reference transcriptome for NRO and initial insights into the genomic responses of NRO to O3. Gene expression profiling reveals altered primary and secondary metabolism of NRO seedlings, including known defense responses such as terpenoid biosynthesis. |
| first_indexed | 2024-10-01T07:30:38Z |
| format | Journal Article |
| id | ntu-10356/146317 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:30:38Z |
| publishDate | 2021 |
| record_format | dspace |
| spelling | ntu-10356/1463172021-02-13T20:15:58Z Transcriptome profiles of Quercus rubra responding to increased O3 stress Soltani, Nourolah Best, Teo Grace, Dantria Nelms, Christen Shumaker, Ketia Romero-Severson, Jeanne Drautz-Moses, Daniela Isabel Schuster, Stephan Christoph Staton, Margaret Carlson, John Gwinn, Kimberly Singapore Centre for Environmental Life Sciences and Engineering Science::Biological sciences Northern Red Oak Transcriptome Background: Climate plays an essential role in forest health, and climate change may increase forest productivity losses due to abiotic and biotic stress. Increased temperature leads to the increased formation of ozone (O3). Ozone is formed by the interaction of sunlight, molecular oxygen and by the reactions of chemicals commonly found in industrial and automobile emissions such as nitrogen oxides and volatile organic compounds. Although it is well known that productivity of Northern red oak (Quercus rubra) (NRO), an ecologically and economically important species in the forests of eastern North America, is reduced by exposure to O3, limited information is available on its responses to exogenous stimuli at the level of gene expression. Results: RNA sequencing yielded more than 323 million high-quality raw sequence reads. De novo assembly generated 52,662 unigenes, of which more than 42,000 sequences could be annotated through homology-based searches. A total of 4140 differential expressed genes (DEGs) were detected in response to O3 stress, as compared to their respective controls. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses of the O3-response DEGs revealed perturbation of several biological pathways including energy, lipid, amino acid, carbohydrate and terpenoid metabolism as well as plant-pathogen interaction. Conclusion: This study provides the first reference transcriptome for NRO and initial insights into the genomic responses of NRO to O3. Gene expression profiling reveals altered primary and secondary metabolism of NRO seedlings, including known defense responses such as terpenoid biosynthesis. Published version 2021-02-09T05:42:21Z 2021-02-09T05:42:21Z 2020 Journal Article Soltani, N., Best, T., Grace, D., Nelms, C., Shumaker, K., Romero-Severson, J., . . . Gwinn, K. (2020). Transcriptome profiles of Quercus rubra responding to increased O3 stress. BMC Genomics, 21(1), 160-. doi:10.1186/s12864-020-6549-5 1471-2164 0000-0003-2971-9353 https://hdl.handle.net/10356/146317 10.1186/s12864-020-6549-5 32059640 2-s2.0-85079534220 1 21 en BMC Genomics © 2020 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. application/pdf |
| spellingShingle | Science::Biological sciences Northern Red Oak Transcriptome Soltani, Nourolah Best, Teo Grace, Dantria Nelms, Christen Shumaker, Ketia Romero-Severson, Jeanne Drautz-Moses, Daniela Isabel Schuster, Stephan Christoph Staton, Margaret Carlson, John Gwinn, Kimberly Transcriptome profiles of Quercus rubra responding to increased O3 stress |
| title | Transcriptome profiles of Quercus rubra responding to increased O3 stress |
| title_full | Transcriptome profiles of Quercus rubra responding to increased O3 stress |
| title_fullStr | Transcriptome profiles of Quercus rubra responding to increased O3 stress |
| title_full_unstemmed | Transcriptome profiles of Quercus rubra responding to increased O3 stress |
| title_short | Transcriptome profiles of Quercus rubra responding to increased O3 stress |
| title_sort | transcriptome profiles of quercus rubra responding to increased o3 stress |
| topic | Science::Biological sciences Northern Red Oak Transcriptome |
| url | https://hdl.handle.net/10356/146317 |
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