Low-cost method and biochip for measuring the trans-epithelial electrical eesistance (TEER) of esophageal epithelium
Trans-epithelial electrical resistance (TEER) is a good indicator of the barrier integrity of epithelial tissues and is often employed in biomedical research as an effective tool to assess ion transport and permeability of tight junctions. The Ussing chamber is the gold standard for measuring TEER o...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2021
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| Online Access: | https://hdl.handle.net/10356/146336 |
| _version_ | 1826123196690071552 |
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| author | Poenar, Daniel Puiu Yang, Guang Wan, Wei Keat Feng, Shilun |
| author2 | School of Electrical and Electronic Engineering |
| author_facet | School of Electrical and Electronic Engineering Poenar, Daniel Puiu Yang, Guang Wan, Wei Keat Feng, Shilun |
| author_sort | Poenar, Daniel Puiu |
| collection | NTU |
| description | Trans-epithelial electrical resistance (TEER) is a good indicator of the barrier integrity of epithelial tissues and is often employed in biomedical research as an effective tool to assess ion transport and permeability of tight junctions. The Ussing chamber is the gold standard for measuring TEER of tissue specimens, but it has major drawbacks: it is a macroscopic method that requires a careful and labor intensive sample mounting protocol, allows a very limited viability for the mounted sample, has large parasitic components and low throughput as it cannot perform multiple simultaneous measurements, and this sophisticated and delicate apparatus has a relatively high cost. This paper demonstrates a low-cost home-made "sandwich ring" method which was used to measure the TEER of tissue specimens effectively. This method inspired the subsequent design of a biochip fabricated using standard soft lithography and laser engraving technologies, with which the TEER of pig epithelial tissues was measured. Moreover, it was possible to temporarily preserve the tissue specimens for days in the biochip and monitor the TEER continuously. Tissue responses after exposure tests to media of various pH values were also successfully recorded using the biochip. All these demonstrate that this biochip could be an effective, cheaper, and easier to use Ussing chamber substitute that may have relevant applications in clinical practice. |
| first_indexed | 2024-10-01T06:00:42Z |
| format | Journal Article |
| id | ntu-10356/146336 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:00:42Z |
| publishDate | 2021 |
| record_format | dspace |
| spelling | ntu-10356/1463362021-02-09T09:13:01Z Low-cost method and biochip for measuring the trans-epithelial electrical eesistance (TEER) of esophageal epithelium Poenar, Daniel Puiu Yang, Guang Wan, Wei Keat Feng, Shilun School of Electrical and Electronic Engineering Centre for Bio Devices and Signal Analysis (VALENS) Engineering::Electrical and electronic engineering TEER Measurement Tissue Biochip Trans-epithelial electrical resistance (TEER) is a good indicator of the barrier integrity of epithelial tissues and is often employed in biomedical research as an effective tool to assess ion transport and permeability of tight junctions. The Ussing chamber is the gold standard for measuring TEER of tissue specimens, but it has major drawbacks: it is a macroscopic method that requires a careful and labor intensive sample mounting protocol, allows a very limited viability for the mounted sample, has large parasitic components and low throughput as it cannot perform multiple simultaneous measurements, and this sophisticated and delicate apparatus has a relatively high cost. This paper demonstrates a low-cost home-made "sandwich ring" method which was used to measure the TEER of tissue specimens effectively. This method inspired the subsequent design of a biochip fabricated using standard soft lithography and laser engraving technologies, with which the TEER of pig epithelial tissues was measured. Moreover, it was possible to temporarily preserve the tissue specimens for days in the biochip and monitor the TEER continuously. Tissue responses after exposure tests to media of various pH values were also successfully recorded using the biochip. All these demonstrate that this biochip could be an effective, cheaper, and easier to use Ussing chamber substitute that may have relevant applications in clinical practice. Ministry of Education (MOE) Published version This research was supported by the Ministry of Education, Singapore, under its Academic ResearchFund Tier 1, Project ID: 2017-T1-002-080, grant/project number RG 151/17. 2021-02-09T09:13:00Z 2021-02-09T09:13:00Z 2020 Journal Article Poenar, D. P., Yang, G., Wan, W. K., & Feng, S. (2020). Low-Cost Method and Biochip for Measuring the Trans-Epithelial Electrical Resistance (TEER) of Esophageal Epithelium. Materials, 13(10), 2354-. doi:10.3390/ma13102354 1996-1944 0000-0003-2971-3517 https://hdl.handle.net/10356/146336 10.3390/ma13102354 32443854 2-s2.0-85085598860 10 13 en 2017-T1-002-080 RG 151/17 Materials © 2020 The Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution(CC BY) license (http://creativecommons.org/licenses/by/4.0/) application/pdf |
| spellingShingle | Engineering::Electrical and electronic engineering TEER Measurement Tissue Biochip Poenar, Daniel Puiu Yang, Guang Wan, Wei Keat Feng, Shilun Low-cost method and biochip for measuring the trans-epithelial electrical eesistance (TEER) of esophageal epithelium |
| title | Low-cost method and biochip for measuring the trans-epithelial electrical eesistance (TEER) of esophageal epithelium |
| title_full | Low-cost method and biochip for measuring the trans-epithelial electrical eesistance (TEER) of esophageal epithelium |
| title_fullStr | Low-cost method and biochip for measuring the trans-epithelial electrical eesistance (TEER) of esophageal epithelium |
| title_full_unstemmed | Low-cost method and biochip for measuring the trans-epithelial electrical eesistance (TEER) of esophageal epithelium |
| title_short | Low-cost method and biochip for measuring the trans-epithelial electrical eesistance (TEER) of esophageal epithelium |
| title_sort | low cost method and biochip for measuring the trans epithelial electrical eesistance teer of esophageal epithelium |
| topic | Engineering::Electrical and electronic engineering TEER Measurement Tissue Biochip |
| url | https://hdl.handle.net/10356/146336 |
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