Colonic mucosal microbiota and association of bacterial taxa with the expression of host antimicrobial peptides in pediatric ulcerative colitis
Inflammatory bowel diseases (IBD), ulcerative colitis (UC) and Crohn's disease (CD), are chronic debilitating disorders of unknown etiology. Over 200 genetic risk loci are associated with IBD, highlighting a key role for immunological and epithelial barrier functions. Environmental factors acco...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2021
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| Online Access: | https://hdl.handle.net/10356/146353 |
| _version_ | 1824454133751480320 |
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| author | Jalanka, Jonna Cheng, Jing Hiippala, Kaisa Ritari, Jarmo Salojärvi, Jarkko Ruuska, Tarja Kalliomäki, Marko Satokari, Reetta |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Jalanka, Jonna Cheng, Jing Hiippala, Kaisa Ritari, Jarmo Salojärvi, Jarkko Ruuska, Tarja Kalliomäki, Marko Satokari, Reetta |
| author_sort | Jalanka, Jonna |
| collection | NTU |
| description | Inflammatory bowel diseases (IBD), ulcerative colitis (UC) and Crohn's disease (CD), are chronic debilitating disorders of unknown etiology. Over 200 genetic risk loci are associated with IBD, highlighting a key role for immunological and epithelial barrier functions. Environmental factors account for the growing incidence of IBD, and microbiota are considered as an important contributor. Microbiota dysbiosis can lead to a loss of tolerogenic immune effects and initiate or exacerbate inflammation. We aimed to study colonic mucosal microbiota and the expression of selected host genes in pediatric UC. We used high-throughput 16S rDNA sequencing to profile microbiota in colonic biopsies of pediatric UC patients (n = 26) and non-IBD controls (n = 27). The expression of 13 genes, including five for antimicrobial peptides, in parallel biopsies was assessed with qRT-PCR. The composition of microbiota between UC and non-IBD differed significantly (PCoA, p = 0.001). UC children had a decrease in Bacteroidetes and an increase in several family-level taxa including Peptostreptococcaceae and Enterobacteriaceae, which correlated negatively with the expression of antimicrobial peptides REG3G and DEFB1, respectively. Enterobacteriaceae correlated positively with the expression siderophore binding protein LCN2 and Betaproteobacteria negatively with DEFB4A expression. The results indicate that reciprocal interaction of epithelial microbiota and defense mechanisms play a role in UC. |
| first_indexed | 2025-02-19T03:17:28Z |
| format | Journal Article |
| id | ntu-10356/146353 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:17:28Z |
| publishDate | 2021 |
| record_format | dspace |
| spelling | ntu-10356/1463532023-02-28T16:57:43Z Colonic mucosal microbiota and association of bacterial taxa with the expression of host antimicrobial peptides in pediatric ulcerative colitis Jalanka, Jonna Cheng, Jing Hiippala, Kaisa Ritari, Jarmo Salojärvi, Jarkko Ruuska, Tarja Kalliomäki, Marko Satokari, Reetta School of Biological Sciences Science::Biological sciences Inflammatory Bowel Disease Ulcerative Colitis Inflammatory bowel diseases (IBD), ulcerative colitis (UC) and Crohn's disease (CD), are chronic debilitating disorders of unknown etiology. Over 200 genetic risk loci are associated with IBD, highlighting a key role for immunological and epithelial barrier functions. Environmental factors account for the growing incidence of IBD, and microbiota are considered as an important contributor. Microbiota dysbiosis can lead to a loss of tolerogenic immune effects and initiate or exacerbate inflammation. We aimed to study colonic mucosal microbiota and the expression of selected host genes in pediatric UC. We used high-throughput 16S rDNA sequencing to profile microbiota in colonic biopsies of pediatric UC patients (n = 26) and non-IBD controls (n = 27). The expression of 13 genes, including five for antimicrobial peptides, in parallel biopsies was assessed with qRT-PCR. The composition of microbiota between UC and non-IBD differed significantly (PCoA, p = 0.001). UC children had a decrease in Bacteroidetes and an increase in several family-level taxa including Peptostreptococcaceae and Enterobacteriaceae, which correlated negatively with the expression of antimicrobial peptides REG3G and DEFB1, respectively. Enterobacteriaceae correlated positively with the expression siderophore binding protein LCN2 and Betaproteobacteria negatively with DEFB4A expression. The results indicate that reciprocal interaction of epithelial microbiota and defense mechanisms play a role in UC. Published version 2021-02-10T08:27:53Z 2021-02-10T08:27:53Z 2020 Journal Article Jalanka, J., Cheng, J., Hiippala, K., Ritari, J., Salojärvi, J., Ruuska, T., . . . Satokari, R. (2020). Colonic Mucosal Microbiota and Association of Bacterial Taxa with the Expression of Host Antimicrobial Peptides in Pediatric Ulcerative Colitis. International Journal of Molecular Sciences, 21(17), 6044-. doi:10.3390/ijms21176044 1661-6596 https://hdl.handle.net/10356/146353 10.3390/ijms21176044 32842596 2-s2.0-85089933776 17 21 en International journal of molecular sciences © 2020 The Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). application/pdf |
| spellingShingle | Science::Biological sciences Inflammatory Bowel Disease Ulcerative Colitis Jalanka, Jonna Cheng, Jing Hiippala, Kaisa Ritari, Jarmo Salojärvi, Jarkko Ruuska, Tarja Kalliomäki, Marko Satokari, Reetta Colonic mucosal microbiota and association of bacterial taxa with the expression of host antimicrobial peptides in pediatric ulcerative colitis |
| title | Colonic mucosal microbiota and association of bacterial taxa with the expression of host antimicrobial peptides in pediatric ulcerative colitis |
| title_full | Colonic mucosal microbiota and association of bacterial taxa with the expression of host antimicrobial peptides in pediatric ulcerative colitis |
| title_fullStr | Colonic mucosal microbiota and association of bacterial taxa with the expression of host antimicrobial peptides in pediatric ulcerative colitis |
| title_full_unstemmed | Colonic mucosal microbiota and association of bacterial taxa with the expression of host antimicrobial peptides in pediatric ulcerative colitis |
| title_short | Colonic mucosal microbiota and association of bacterial taxa with the expression of host antimicrobial peptides in pediatric ulcerative colitis |
| title_sort | colonic mucosal microbiota and association of bacterial taxa with the expression of host antimicrobial peptides in pediatric ulcerative colitis |
| topic | Science::Biological sciences Inflammatory Bowel Disease Ulcerative Colitis |
| url | https://hdl.handle.net/10356/146353 |
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