A pilot study investigating anterior segment optical coherence tomography angiography as a non-invasive tool in evaluating corneal vascularisation
The current assessment of corneal vascularisation (CV) relies on slit-lamp examination, which may be subjective. Dye-based angiographies, like indocyanine green angiography (ICGA), allows for good visualisation of anterior segment blood vessels. However, ICGA is invasive and can be associated with s...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2021
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| Online Access: | https://hdl.handle.net/10356/146420 |
| _version_ | 1826109910594617344 |
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| author | Ong, Hon Shing Tey, Kai Yuan Ke, Mengyuan Tan, Bingyao Chua, Jacqueline Schmetterer, Leopold Mehta, Jodhbir Singh Ang, Marcus |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Ong, Hon Shing Tey, Kai Yuan Ke, Mengyuan Tan, Bingyao Chua, Jacqueline Schmetterer, Leopold Mehta, Jodhbir Singh Ang, Marcus |
| author_sort | Ong, Hon Shing |
| collection | NTU |
| description | The current assessment of corneal vascularisation (CV) relies on slit-lamp examination, which may be subjective. Dye-based angiographies, like indocyanine green angiography (ICGA), allows for good visualisation of anterior segment blood vessels. However, ICGA is invasive and can be associated with systemic adverse effects. Anterior segment optical coherence tomography angiography (AS-OCTA) is a non-invasive tool that has been shown to successfully delineate CV. However, there are no previous studies that have reported if AS-OCTA can determine CV stage and activity. We used an established CV model in rabbits to examine serial AS-OCTA scans of CV development and regression following treatment with anti-vascular endothelial growth factor. We compared AS-OCTA derived vascular measurements to that of ICGA determined vessel leakage and CV staging. Our results showed that AS-OCTA vessel densities and vessel branch area significantly correlated with the severity of CV based on ICGA (all p ≤ 0.05). We also found that AS-OCTA vessel densities correlated with ICGA vessel leakage time, following an inverse linear relationship (r2 = - 0.726, p < 0.01). Changes in aqueous levels of CXCL-12 and PIGF cytokines significantly correlated with AS-OCTA vessel densities (r2 = 0.736 and r2 = 0.731 respectively, all p < 0.05). In summary, we found that AS-OCTA derived vessel parameters may be useful for assessing CV severity, while vessel density correlates with CV activity and leakage. Thus, our pilot animal model study suggests that AS-OCTA may be a useful non-invasive imaging tool to provide objective assessment of CV to examine progression or response in treatment, which requires confirmation in clinical studies. |
| first_indexed | 2024-10-01T02:25:49Z |
| format | Journal Article |
| id | ntu-10356/146420 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:25:49Z |
| publishDate | 2021 |
| record_format | dspace |
| spelling | ntu-10356/1464202023-12-29T06:45:14Z A pilot study investigating anterior segment optical coherence tomography angiography as a non-invasive tool in evaluating corneal vascularisation Ong, Hon Shing Tey, Kai Yuan Ke, Mengyuan Tan, Bingyao Chua, Jacqueline Schmetterer, Leopold Mehta, Jodhbir Singh Ang, Marcus School of Chemical and Biomedical Engineering School of Materials Science and Engineering Engineering::Chemical engineering Eye Diseases Eye Manifestations The current assessment of corneal vascularisation (CV) relies on slit-lamp examination, which may be subjective. Dye-based angiographies, like indocyanine green angiography (ICGA), allows for good visualisation of anterior segment blood vessels. However, ICGA is invasive and can be associated with systemic adverse effects. Anterior segment optical coherence tomography angiography (AS-OCTA) is a non-invasive tool that has been shown to successfully delineate CV. However, there are no previous studies that have reported if AS-OCTA can determine CV stage and activity. We used an established CV model in rabbits to examine serial AS-OCTA scans of CV development and regression following treatment with anti-vascular endothelial growth factor. We compared AS-OCTA derived vascular measurements to that of ICGA determined vessel leakage and CV staging. Our results showed that AS-OCTA vessel densities and vessel branch area significantly correlated with the severity of CV based on ICGA (all p ≤ 0.05). We also found that AS-OCTA vessel densities correlated with ICGA vessel leakage time, following an inverse linear relationship (r2 = - 0.726, p < 0.01). Changes in aqueous levels of CXCL-12 and PIGF cytokines significantly correlated with AS-OCTA vessel densities (r2 = 0.736 and r2 = 0.731 respectively, all p < 0.05). In summary, we found that AS-OCTA derived vessel parameters may be useful for assessing CV severity, while vessel density correlates with CV activity and leakage. Thus, our pilot animal model study suggests that AS-OCTA may be a useful non-invasive imaging tool to provide objective assessment of CV to examine progression or response in treatment, which requires confirmation in clinical studies. National Medical Research Council (NMRC) Published version This work was funded by grants from the National Medical Research Council (CG/C010A/2017; OFIRG/0048/2017; and OFLCG/004c/2018), Singapore. 2021-02-16T08:25:02Z 2021-02-16T08:25:02Z 2021 Journal Article Ong, H. S., Tey, K. Y., Ke, M., Tan, B., Chua, J., Schmetterer, L., . . . Ang, M. (2021). A pilot study investigating anterior segment optical coherence tomography angiography as a non-invasive tool in evaluating corneal vascularisation. Scientific Reports, 11(1), 1212-. doi:10.1038/s41598-020-80099-2 2045-2322 https://hdl.handle.net/10356/146420 10.1038/s41598-020-80099-2 33441810 2-s2.0-85099246977 1 11 en CG/C010A/2017 OFIRG/0048/2017 OFLCG/004c/2018 Scientific Reports © 2021 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. application/pdf |
| spellingShingle | Engineering::Chemical engineering Eye Diseases Eye Manifestations Ong, Hon Shing Tey, Kai Yuan Ke, Mengyuan Tan, Bingyao Chua, Jacqueline Schmetterer, Leopold Mehta, Jodhbir Singh Ang, Marcus A pilot study investigating anterior segment optical coherence tomography angiography as a non-invasive tool in evaluating corneal vascularisation |
| title | A pilot study investigating anterior segment optical coherence tomography angiography as a non-invasive tool in evaluating corneal vascularisation |
| title_full | A pilot study investigating anterior segment optical coherence tomography angiography as a non-invasive tool in evaluating corneal vascularisation |
| title_fullStr | A pilot study investigating anterior segment optical coherence tomography angiography as a non-invasive tool in evaluating corneal vascularisation |
| title_full_unstemmed | A pilot study investigating anterior segment optical coherence tomography angiography as a non-invasive tool in evaluating corneal vascularisation |
| title_short | A pilot study investigating anterior segment optical coherence tomography angiography as a non-invasive tool in evaluating corneal vascularisation |
| title_sort | pilot study investigating anterior segment optical coherence tomography angiography as a non invasive tool in evaluating corneal vascularisation |
| topic | Engineering::Chemical engineering Eye Diseases Eye Manifestations |
| url | https://hdl.handle.net/10356/146420 |
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