Characterization of anisotropic human hair keratin scaffolds fabricated via directed ice templating
Human hair keratin (HHK) is successfully exploited as raw materials for 3D scaffolds for soft tissue regeneration owing to its excellent biocompatibility and bioactivity. However, most HHK scaffolds are not able to achieve the anisotropic mechanical properties of soft tissues such as tendons and lig...
| Main Authors: | , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2021
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| Online Access: | https://hdl.handle.net/10356/146429 |
| _version_ | 1826111104815726592 |
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| author | Zhao, Zhitong Moay, Zi Kuang Lai, Hui Ying Goh, Bernice Huan Rong Chua, Huei Min Setyawati, Magdiel Inggrid Ng, Kee Woei |
| author2 | School of Materials Science and Engineering |
| author_facet | School of Materials Science and Engineering Zhao, Zhitong Moay, Zi Kuang Lai, Hui Ying Goh, Bernice Huan Rong Chua, Huei Min Setyawati, Magdiel Inggrid Ng, Kee Woei |
| author_sort | Zhao, Zhitong |
| collection | NTU |
| description | Human hair keratin (HHK) is successfully exploited as raw materials for 3D scaffolds for soft tissue regeneration owing to its excellent biocompatibility and bioactivity. However, most HHK scaffolds are not able to achieve the anisotropic mechanical properties of soft tissues such as tendons and ligaments due to lack of tunable, well-defined microstructures. In this study, directed ice templating method is used to fabricate anisotropic HHK scaffolds that are characterized by aligned pores (channels) in between keratin layers in the longitudinal plane. In contrast, pores in the transverse plane maintain a homogenous rounded morphology. Channel widths throughout the scaffolds range from ≈5 to ≈15 µm and are tunable by varying the freezing temperature. In comparison with HHK scaffolds with random, isotropic pore structures, the tensile strength of anisotropic HHK scaffolds is enhanced significantly by up to fourfolds (≈200 to ≈800 kPa) when the tensile load is applied in the direction parallel to the aligned pores. In vitro results demonstrate that the anisotropic HHK scaffolds are able to support human dermal fibroblast adhesion, spreading, and proliferation. The findings suggest that HHK scaffolds with well-defined, aligned microstructure hold promise as templates for soft tissues regeneration by mimicking their anisotropic properties. |
| first_indexed | 2024-10-01T02:45:31Z |
| format | Journal Article |
| id | ntu-10356/146429 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:45:31Z |
| publishDate | 2021 |
| record_format | dspace |
| spelling | ntu-10356/1464292023-07-14T15:52:35Z Characterization of anisotropic human hair keratin scaffolds fabricated via directed ice templating Zhao, Zhitong Moay, Zi Kuang Lai, Hui Ying Goh, Bernice Huan Rong Chua, Huei Min Setyawati, Magdiel Inggrid Ng, Kee Woei School of Materials Science and Engineering Engineering::Materials::Biomaterials Human Hair Keratin Ice Templating Method Human hair keratin (HHK) is successfully exploited as raw materials for 3D scaffolds for soft tissue regeneration owing to its excellent biocompatibility and bioactivity. However, most HHK scaffolds are not able to achieve the anisotropic mechanical properties of soft tissues such as tendons and ligaments due to lack of tunable, well-defined microstructures. In this study, directed ice templating method is used to fabricate anisotropic HHK scaffolds that are characterized by aligned pores (channels) in between keratin layers in the longitudinal plane. In contrast, pores in the transverse plane maintain a homogenous rounded morphology. Channel widths throughout the scaffolds range from ≈5 to ≈15 µm and are tunable by varying the freezing temperature. In comparison with HHK scaffolds with random, isotropic pore structures, the tensile strength of anisotropic HHK scaffolds is enhanced significantly by up to fourfolds (≈200 to ≈800 kPa) when the tensile load is applied in the direction parallel to the aligned pores. In vitro results demonstrate that the anisotropic HHK scaffolds are able to support human dermal fibroblast adhesion, spreading, and proliferation. The findings suggest that HHK scaffolds with well-defined, aligned microstructure hold promise as templates for soft tissues regeneration by mimicking their anisotropic properties. Agency for Science, Technology and Research (A*STAR) Accepted version This research is supported by the Agency for Science, Technology and Research (A*STAR) under its Acne and Sebaceous Gland Program & Wound Care Innovation for the Tropics IAF-PP (H17/01/a0/008 & H17/01/a0/0L9). 2021-02-17T00:52:44Z 2021-02-17T00:52:44Z 2021 Journal Article Zhao, Z., Moay, Z. K., Lai, H. Y., Goh, B. H. R., Chua, H. M., Setyawati, M. I., & Ng, K. W. (2021). Characterization of anisotropic human hair keratin scaffolds fabricated via directed ice templating. Macromolecular Bioscience, 21(2), e2000314-. doi:10.1002/mabi.202000314 1616-5187 https://hdl.handle.net/10356/146429 10.1002/mabi.202000314 33146949 2-s2.0-85096754676 2 21 e2000314 en Macromolecular Bioscience This is the accepted version of the following article: Zhao, Z., Moay, Z. K., Lai, H. Y., Goh, B. H. R., Chua, H. M., Setyawati, M. I., & Ng, K. W. (2021). Characterization of anisotropic human hair keratin scaffolds fabricated via directed ice templating. Macromolecular Bioscience, 21(2), e2000314-. doi:10.1002/mabi.202000314, which has been published in final form at https://doi.org/10.1002/mabi.202000314. This article may be used for non-commercial purposes in accordance with the Wiley Self-Archiving Policy [https://authorservices.wiley.com/authorresources/Journal-Authors/licensing/self-archiving.html]. application/pdf |
| spellingShingle | Engineering::Materials::Biomaterials Human Hair Keratin Ice Templating Method Zhao, Zhitong Moay, Zi Kuang Lai, Hui Ying Goh, Bernice Huan Rong Chua, Huei Min Setyawati, Magdiel Inggrid Ng, Kee Woei Characterization of anisotropic human hair keratin scaffolds fabricated via directed ice templating |
| title | Characterization of anisotropic human hair keratin scaffolds fabricated via directed ice templating |
| title_full | Characterization of anisotropic human hair keratin scaffolds fabricated via directed ice templating |
| title_fullStr | Characterization of anisotropic human hair keratin scaffolds fabricated via directed ice templating |
| title_full_unstemmed | Characterization of anisotropic human hair keratin scaffolds fabricated via directed ice templating |
| title_short | Characterization of anisotropic human hair keratin scaffolds fabricated via directed ice templating |
| title_sort | characterization of anisotropic human hair keratin scaffolds fabricated via directed ice templating |
| topic | Engineering::Materials::Biomaterials Human Hair Keratin Ice Templating Method |
| url | https://hdl.handle.net/10356/146429 |
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