Biomolecular condensation-mediated actin remodeling in plant innate immunity
Upon bacterial invasion, the plant host acutely remodels the actin cytoskeleton to coordinate defense mechanisms. However, the molecular mechanisms by which plant triggers rapid actin remodeling remain enigmatic. In my PhD study, we have found that plant actin nucleation factor, type-I formin, serve...
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| 格式: | Thesis-Doctor of Philosophy |
| 语言: | English |
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Nanyang Technological University
2021
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| 主题: | |
| 在线阅读: | https://hdl.handle.net/10356/146511 |
| _version_ | 1826114980902076416 |
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| author | Ma, Zhiming |
| author2 | Miao Yansong |
| author_facet | Miao Yansong Ma, Zhiming |
| author_sort | Ma, Zhiming |
| collection | NTU |
| description | Upon bacterial invasion, the plant host acutely remodels the actin cytoskeleton to coordinate defense mechanisms. However, the molecular mechanisms by which plant triggers rapid actin remodeling remain enigmatic. In my PhD study, we have found that plant actin nucleation factor, type-I formin, serves as the molecular sensor that responds to bacterial virulence factors for actin remodeling during host immune signalling. Two types of virulence factors of Xanthomonas campestris pv. campestris (Xcc), diffusible signal factor (DSF), and pathogen-associated molecular pattern (PAMP), flagellin (flg22), regulate actin assembly through oppositely tuning the type-I formin nano-clustering on the plasma membrane (PM). The formin nano-clustering and nucleation activity depend highly on the mechanical regulation by the cell wall-PM-actin cytoskeleton continuum during both DSF and PAMP signalling. Specifically, DSF attenuates formin nano-clustering and causes reduced actin assembly by perturbing the integrity of cell wall. While PAMPs trigger the nano-clustering of formin in a PM nanodomain assembly factor Remorin-dependent manner. Together, our results have unravelled a central molecular mechanism for rapid actin remodeling during plant-bacteria interactions, in which bacterial signalling molecules finetune plant type-I formin condensation in a host mechanical structure-dependent manner. |
| first_indexed | 2024-10-01T03:48:04Z |
| format | Thesis-Doctor of Philosophy |
| id | ntu-10356/146511 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:48:04Z |
| publishDate | 2021 |
| publisher | Nanyang Technological University |
| record_format | dspace |
| spelling | ntu-10356/1465112023-02-28T18:35:44Z Biomolecular condensation-mediated actin remodeling in plant innate immunity Ma, Zhiming Miao Yansong School of Biological Sciences Arabidopsis yansongm@ntu.edu.sg Science::Biological sciences::Botany Science::Biological sciences::Biochemistry Upon bacterial invasion, the plant host acutely remodels the actin cytoskeleton to coordinate defense mechanisms. However, the molecular mechanisms by which plant triggers rapid actin remodeling remain enigmatic. In my PhD study, we have found that plant actin nucleation factor, type-I formin, serves as the molecular sensor that responds to bacterial virulence factors for actin remodeling during host immune signalling. Two types of virulence factors of Xanthomonas campestris pv. campestris (Xcc), diffusible signal factor (DSF), and pathogen-associated molecular pattern (PAMP), flagellin (flg22), regulate actin assembly through oppositely tuning the type-I formin nano-clustering on the plasma membrane (PM). The formin nano-clustering and nucleation activity depend highly on the mechanical regulation by the cell wall-PM-actin cytoskeleton continuum during both DSF and PAMP signalling. Specifically, DSF attenuates formin nano-clustering and causes reduced actin assembly by perturbing the integrity of cell wall. While PAMPs trigger the nano-clustering of formin in a PM nanodomain assembly factor Remorin-dependent manner. Together, our results have unravelled a central molecular mechanism for rapid actin remodeling during plant-bacteria interactions, in which bacterial signalling molecules finetune plant type-I formin condensation in a host mechanical structure-dependent manner. Doctor of Philosophy 2021-02-23T01:22:20Z 2021-02-23T01:22:20Z 2021 Thesis-Doctor of Philosophy Ma, Z. (2021). Biomolecular condensation-mediated actin remodeling in plant innate immunity. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/146511 10.32657/10356/146511 en This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0). application/pdf Nanyang Technological University |
| spellingShingle | Science::Biological sciences::Botany Science::Biological sciences::Biochemistry Ma, Zhiming Biomolecular condensation-mediated actin remodeling in plant innate immunity |
| title | Biomolecular condensation-mediated actin remodeling in plant innate immunity |
| title_full | Biomolecular condensation-mediated actin remodeling in plant innate immunity |
| title_fullStr | Biomolecular condensation-mediated actin remodeling in plant innate immunity |
| title_full_unstemmed | Biomolecular condensation-mediated actin remodeling in plant innate immunity |
| title_short | Biomolecular condensation-mediated actin remodeling in plant innate immunity |
| title_sort | biomolecular condensation mediated actin remodeling in plant innate immunity |
| topic | Science::Biological sciences::Botany Science::Biological sciences::Biochemistry |
| url | https://hdl.handle.net/10356/146511 |
| work_keys_str_mv | AT mazhiming biomolecularcondensationmediatedactinremodelinginplantinnateimmunity |