Solid-state NMR studies of chromatin structure and dynamics
Understanding the dynamic organization of chromatin is indispensable as it provides crucial information to delineate cellular processes that it mediates–DNA replication, transcription, and repair. Nucleosome core particle (NCP) composed of core histones, organizing ~147 bp of DNA constitutes the fun...
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| Format: | Thesis-Doctor of Philosophy |
| Language: | English |
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Nanyang Technological University
2021
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| Online Access: | https://hdl.handle.net/10356/146519 |
| _version_ | 1811691243447517184 |
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| author | Prasanna, Chinmayi |
| author2 | Lars Nordenskiöld |
| author_facet | Lars Nordenskiöld Prasanna, Chinmayi |
| author_sort | Prasanna, Chinmayi |
| collection | NTU |
| description | Understanding the dynamic organization of chromatin is indispensable as it provides crucial information to delineate cellular processes that it mediates–DNA replication, transcription, and repair. Nucleosome core particle (NCP) composed of core histones, organizing ~147 bp of DNA constitutes the fundamental unit of eukaryotic chromatin. While the structure of nucleosome is investigated predominantly using X-ray crystallography, quantitative information about nucleosome dynamics remains elusive. Our objective is to address this gap using solid-state nuclear magnetic resonance (SSNMR) spectroscopy, which is a state-of-the-art technology to probe protein dynamics at atomic resolution. We have conducted SSNMR experiments for human histones in the NCP at physiological chromatin concentrations to probe the site-specific motions and amplitudes. We also investigated the influence of histone dynamics in the context of nucleosomes in the chromatin fiber and the human telomeric repeat DNA sequence. Our study is the first to have probed quantitative motions of histones in the chromatin. |
| first_indexed | 2024-10-01T06:16:48Z |
| format | Thesis-Doctor of Philosophy |
| id | ntu-10356/146519 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:16:48Z |
| publishDate | 2021 |
| publisher | Nanyang Technological University |
| record_format | dspace |
| spelling | ntu-10356/1465192023-02-28T18:43:23Z Solid-state NMR studies of chromatin structure and dynamics Prasanna, Chinmayi Lars Nordenskiöld School of Biological Sciences LarsNor@ntu.edu.sg Science::Biological sciences Understanding the dynamic organization of chromatin is indispensable as it provides crucial information to delineate cellular processes that it mediates–DNA replication, transcription, and repair. Nucleosome core particle (NCP) composed of core histones, organizing ~147 bp of DNA constitutes the fundamental unit of eukaryotic chromatin. While the structure of nucleosome is investigated predominantly using X-ray crystallography, quantitative information about nucleosome dynamics remains elusive. Our objective is to address this gap using solid-state nuclear magnetic resonance (SSNMR) spectroscopy, which is a state-of-the-art technology to probe protein dynamics at atomic resolution. We have conducted SSNMR experiments for human histones in the NCP at physiological chromatin concentrations to probe the site-specific motions and amplitudes. We also investigated the influence of histone dynamics in the context of nucleosomes in the chromatin fiber and the human telomeric repeat DNA sequence. Our study is the first to have probed quantitative motions of histones in the chromatin. Doctor of Philosophy 2021-02-23T05:40:53Z 2021-02-23T05:40:53Z 2020 Thesis-Doctor of Philosophy Prasanna, C. (2020). Solid-state NMR studies of chromatin structure and dynamics. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/146519 10.32657/10356/146519 en This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0). application/pdf Nanyang Technological University |
| spellingShingle | Science::Biological sciences Prasanna, Chinmayi Solid-state NMR studies of chromatin structure and dynamics |
| title | Solid-state NMR studies of chromatin structure and dynamics |
| title_full | Solid-state NMR studies of chromatin structure and dynamics |
| title_fullStr | Solid-state NMR studies of chromatin structure and dynamics |
| title_full_unstemmed | Solid-state NMR studies of chromatin structure and dynamics |
| title_short | Solid-state NMR studies of chromatin structure and dynamics |
| title_sort | solid state nmr studies of chromatin structure and dynamics |
| topic | Science::Biological sciences |
| url | https://hdl.handle.net/10356/146519 |
| work_keys_str_mv | AT prasannachinmayi solidstatenmrstudiesofchromatinstructureanddynamics |