Discovery and characterization of hevein-like peptides in functional food and medicinal plants
Hevein-like peptides (HLPs) are a family of cysteine-rich, structurally compact, plant defense peptides. However, they remain underexplored and poorly understood. The objectives of my thesis consist of two parts. The first part focuses on the discovery of novel HLPs and to examine their structure, b...
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| Format: | Thesis-Doctor of Philosophy |
| Language: | English |
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Nanyang Technological University
2021
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| Online Access: | https://hdl.handle.net/10356/146624 |
| _version_ | 1824453584216915968 |
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| author | Tay, Stephanie Victoria Choo Gek |
| author2 | James P Tam |
| author_facet | James P Tam Tay, Stephanie Victoria Choo Gek |
| author_sort | Tay, Stephanie Victoria Choo Gek |
| collection | NTU |
| description | Hevein-like peptides (HLPs) are a family of cysteine-rich, structurally compact, plant defense peptides. However, they remain underexplored and poorly understood. The objectives of my thesis consist of two parts. The first part focuses on the discovery of novel HLPs and to examine their structure, biosynthesis, distribution, occurrence and functions in selected plants. They include two functional grains, Chenopodium quinoa (quinoa), Avena sativa (oats), and a medicinal plant, Eleutherococcus trifoliatus (Three-leaved Eleutherococcus， 苦力心). From these plants, I discovered 13 novel HLPs: three 6C-chitin-binding HLPs, nine 8C-chitin-binding HLPs and a 8C-non-chitin-binding HLP. The second part focuses on data mining using bioinformatics to identify 1204 novel HLPs from 252 plant families. Together this study suggests that HLPs are one of the largest family of CRPs and provides insights into their sequence, molecular and functional diversity, potentially leading to development of novel peptidyl drugs, antimicrobial agents and pathogen-resistant transgenic crops. |
| first_indexed | 2025-02-19T03:08:44Z |
| format | Thesis-Doctor of Philosophy |
| id | ntu-10356/146624 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:08:44Z |
| publishDate | 2021 |
| publisher | Nanyang Technological University |
| record_format | dspace |
| spelling | ntu-10356/1466242023-02-28T18:48:51Z Discovery and characterization of hevein-like peptides in functional food and medicinal plants Tay, Stephanie Victoria Choo Gek James P Tam School of Biological Sciences JPTam@ntu.edu.sg Science::Biological sciences Hevein-like peptides (HLPs) are a family of cysteine-rich, structurally compact, plant defense peptides. However, they remain underexplored and poorly understood. The objectives of my thesis consist of two parts. The first part focuses on the discovery of novel HLPs and to examine their structure, biosynthesis, distribution, occurrence and functions in selected plants. They include two functional grains, Chenopodium quinoa (quinoa), Avena sativa (oats), and a medicinal plant, Eleutherococcus trifoliatus (Three-leaved Eleutherococcus， 苦力心). From these plants, I discovered 13 novel HLPs: three 6C-chitin-binding HLPs, nine 8C-chitin-binding HLPs and a 8C-non-chitin-binding HLP. The second part focuses on data mining using bioinformatics to identify 1204 novel HLPs from 252 plant families. Together this study suggests that HLPs are one of the largest family of CRPs and provides insights into their sequence, molecular and functional diversity, potentially leading to development of novel peptidyl drugs, antimicrobial agents and pathogen-resistant transgenic crops. Doctor of Philosophy 2021-03-03T06:40:58Z 2021-03-03T06:40:58Z 2020 Thesis-Doctor of Philosophy Tay, S. V. C. G. (2020). Discovery and characterization of hevein-like peptides in functional food and medicinal plants. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/146624 10.32657/10356/146624 en This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0). application/pdf Nanyang Technological University |
| spellingShingle | Science::Biological sciences Tay, Stephanie Victoria Choo Gek Discovery and characterization of hevein-like peptides in functional food and medicinal plants |
| title | Discovery and characterization of hevein-like peptides in functional food and medicinal plants |
| title_full | Discovery and characterization of hevein-like peptides in functional food and medicinal plants |
| title_fullStr | Discovery and characterization of hevein-like peptides in functional food and medicinal plants |
| title_full_unstemmed | Discovery and characterization of hevein-like peptides in functional food and medicinal plants |
| title_short | Discovery and characterization of hevein-like peptides in functional food and medicinal plants |
| title_sort | discovery and characterization of hevein like peptides in functional food and medicinal plants |
| topic | Science::Biological sciences |
| url | https://hdl.handle.net/10356/146624 |
| work_keys_str_mv | AT taystephanievictoriachoogek discoveryandcharacterizationofheveinlikepeptidesinfunctionalfoodandmedicinalplants |