Annual phytoplankton dynamics in coastal waters from Fildes Bay, Western Antarctic Peninsula
Year-round reports of phytoplankton dynamics in the West Antarctic Peninsula are rare and mainly limited to microscopy and/or pigment-based studies. We analyzed the phytoplankton community from coastal waters of Fildes Bay in the West Antarctic Peninsula between January 2014 and 2015 using metabarco...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2021
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| Online Access: | https://hdl.handle.net/10356/146646 |
| _version_ | 1811690312122236928 |
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| author | Trefault, Nicole De la Iglesia, Rodrigo Moreno-Pino, Mario Lopes dos Santos, Adriana Gérikas Ribeiro, Catherine Parada-Pozo, Génesis Cristi, Antonia Marie, Dominique Vaulot, Daniel |
| author2 | Asian School of the Environment |
| author_facet | Asian School of the Environment Trefault, Nicole De la Iglesia, Rodrigo Moreno-Pino, Mario Lopes dos Santos, Adriana Gérikas Ribeiro, Catherine Parada-Pozo, Génesis Cristi, Antonia Marie, Dominique Vaulot, Daniel |
| author_sort | Trefault, Nicole |
| collection | NTU |
| description | Year-round reports of phytoplankton dynamics in the West Antarctic Peninsula are rare and mainly limited to microscopy and/or pigment-based studies. We analyzed the phytoplankton community from coastal waters of Fildes Bay in the West Antarctic Peninsula between January 2014 and 2015 using metabarcoding of the nuclear and plastidial 18/16S rRNA gene from both size-fractionated and flow cytometry sorted samples. Overall 14 classes of photosynthetic eukaryotes were present in our samples with the following dominating: Bacillariophyta (diatoms), Pelagophyceae and Dictyochophyceae for division Ochrophyta, Mamiellophyceae and Pyramimonadophyceae for division Chlorophyta, Haptophyta and Cryptophyta. Each metabarcoding approach yielded a different image of the phytoplankton community with for example Prymnesiophyceae more prevalent in plastidial metabarcodes and Mamiellophyceae in nuclear ones. Diatoms were dominant in the larger size fractions and during summer, while Prymnesiophyceae and Cryptophyceae were dominant in colder seasons. Pelagophyceae were particularly abundant towards the end of autumn (May). In addition of Micromonas polaris and Micromonas sp. clade B3, both previously reported in Arctic waters, we detected a new Micromonas 18S rRNA sequence signature, close to, but clearly distinct from M. polaris, which potentially represents a new clade specific of the Antarctic. These results highlight the need for complementary strategies as well as the importance of year-round monitoring for a comprehensive description of phytoplankton communities in Antarctic coastal waters. |
| first_indexed | 2024-10-01T06:01:59Z |
| format | Journal Article |
| id | ntu-10356/146646 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:01:59Z |
| publishDate | 2021 |
| record_format | dspace |
| spelling | ntu-10356/1466462023-02-28T16:41:13Z Annual phytoplankton dynamics in coastal waters from Fildes Bay, Western Antarctic Peninsula Trefault, Nicole De la Iglesia, Rodrigo Moreno-Pino, Mario Lopes dos Santos, Adriana Gérikas Ribeiro, Catherine Parada-Pozo, Génesis Cristi, Antonia Marie, Dominique Vaulot, Daniel Asian School of the Environment Engineering::Environmental engineering Biogeography Biooceanography Year-round reports of phytoplankton dynamics in the West Antarctic Peninsula are rare and mainly limited to microscopy and/or pigment-based studies. We analyzed the phytoplankton community from coastal waters of Fildes Bay in the West Antarctic Peninsula between January 2014 and 2015 using metabarcoding of the nuclear and plastidial 18/16S rRNA gene from both size-fractionated and flow cytometry sorted samples. Overall 14 classes of photosynthetic eukaryotes were present in our samples with the following dominating: Bacillariophyta (diatoms), Pelagophyceae and Dictyochophyceae for division Ochrophyta, Mamiellophyceae and Pyramimonadophyceae for division Chlorophyta, Haptophyta and Cryptophyta. Each metabarcoding approach yielded a different image of the phytoplankton community with for example Prymnesiophyceae more prevalent in plastidial metabarcodes and Mamiellophyceae in nuclear ones. Diatoms were dominant in the larger size fractions and during summer, while Prymnesiophyceae and Cryptophyceae were dominant in colder seasons. Pelagophyceae were particularly abundant towards the end of autumn (May). In addition of Micromonas polaris and Micromonas sp. clade B3, both previously reported in Arctic waters, we detected a new Micromonas 18S rRNA sequence signature, close to, but clearly distinct from M. polaris, which potentially represents a new clade specific of the Antarctic. These results highlight the need for complementary strategies as well as the importance of year-round monitoring for a comprehensive description of phytoplankton communities in Antarctic coastal waters. Ministry of Education (MOE) Published version This work was funded by INACH RG_31-15, INACH RT_34-17 and FONDECYT No. 1190879 grants. Collaboration between Chile and France was funded through ECOS-CONICYT No. C16B02 and CNRS International Research Network “Diversity, Evolution and Biotechnology of Marine Algae” (GDRI No. 0803). Catherine Géri-kas Ribeiro was supported by the FONDECYT Project No. 3190827. The authors thank Dr. Ernesto Molina for sampling during autumn and spring, as well the logistic support at the scientific station Professor Julio Escudero, INACH. Adriana Lopes dos Santos was supported by the Singapore Ministry of Education, Academic Research Fund Tier 1 (RG26/19). We thank the Roscoff ABIMS platform of the FR2424 (CNRS, Sorbonne Université) for bioinformatics resources. 2021-03-04T04:48:34Z 2021-03-04T04:48:34Z 2021 Journal Article Trefault, N., De la Iglesia, R., Moreno-Pino, M., Lopes dos Santos, A., Gérikas Ribeiro, C., Parada-Pozo, G., . . . Vaulot, D. (2021). Annual phytoplankton dynamics in coastal waters from Fildes Bay, Western Antarctic Peninsula. Scientific Reports, 11(1), 1368-. doi:10.1038/s41598-020-80568-8 2045-2322 0000-0002-2000-8697 0000-0002-0736-4937 0000-0003-0531-2313 0000-0003-1381-8170 0000-0002-0717-5685 https://hdl.handle.net/10356/146646 10.1038/s41598-020-80568-8 33446791 2-s2.0-85099466616 1 11 en RG26/19 Scientific Reports © 2021 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. application/pdf |
| spellingShingle | Engineering::Environmental engineering Biogeography Biooceanography Trefault, Nicole De la Iglesia, Rodrigo Moreno-Pino, Mario Lopes dos Santos, Adriana Gérikas Ribeiro, Catherine Parada-Pozo, Génesis Cristi, Antonia Marie, Dominique Vaulot, Daniel Annual phytoplankton dynamics in coastal waters from Fildes Bay, Western Antarctic Peninsula |
| title | Annual phytoplankton dynamics in coastal waters from Fildes Bay, Western Antarctic Peninsula |
| title_full | Annual phytoplankton dynamics in coastal waters from Fildes Bay, Western Antarctic Peninsula |
| title_fullStr | Annual phytoplankton dynamics in coastal waters from Fildes Bay, Western Antarctic Peninsula |
| title_full_unstemmed | Annual phytoplankton dynamics in coastal waters from Fildes Bay, Western Antarctic Peninsula |
| title_short | Annual phytoplankton dynamics in coastal waters from Fildes Bay, Western Antarctic Peninsula |
| title_sort | annual phytoplankton dynamics in coastal waters from fildes bay western antarctic peninsula |
| topic | Engineering::Environmental engineering Biogeography Biooceanography |
| url | https://hdl.handle.net/10356/146646 |
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