Reconstitution of actin polymerization via artificial cells
Unilamellar liposomes are sphere-shaped vesicles which are artificially created from natural phospholipids and/or cholesterol to mimic the composition of bilayer plasma membrane. Giant Unilamellar Vesicles (GUVs) are enclosed compartments or “microreactors” which offers a way to study the effect of...
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| Format: | Thesis-Master by Research |
| Language: | English |
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Nanyang Technological University
2021
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| Online Access: | https://hdl.handle.net/10356/146725 |
| _version_ | 1811677341412229120 |
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| author | Susanti |
| author2 | Miao Yansong |
| author_facet | Miao Yansong Susanti |
| author_sort | Susanti |
| collection | NTU |
| description | Unilamellar liposomes are sphere-shaped vesicles which are artificially created from natural phospholipids and/or cholesterol to mimic the composition of bilayer plasma membrane. Giant Unilamellar Vesicles (GUVs) are enclosed compartments or “microreactors” which offers a way to study the effect of confinement on cellular systems. Here we demonstrated several preparation approaches of GUVs, namely water-in-oil droplets with microfluidics apparatus, one-pot assembly water-in-oil droplets, GUVs with gentle hydration method and GUVs with reverse emulsion method. Specifically, we highlighted the advantages and challenges of each approach in reconstituting actin cytoskeleton, in an attempt to mimic the interaction between actin and actin-binding proteins – AtFH1, AtVLN4, XopR, Sac6 – in the cell-like vesicles. Moreover, we had also showed that we could vary the lipid compositions to create artificial cell of desired properties, as well as manipulating the proteins-lipids interaction on the membrane interface. Overall, we established capabilities of reconstituting actin polymerization inside an artificial cell-like confinement. |
| first_indexed | 2024-10-01T02:35:50Z |
| format | Thesis-Master by Research |
| id | ntu-10356/146725 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:35:50Z |
| publishDate | 2021 |
| publisher | Nanyang Technological University |
| record_format | dspace |
| spelling | ntu-10356/1467252023-02-28T18:31:57Z Reconstitution of actin polymerization via artificial cells Susanti Miao Yansong School of Biological Sciences yansongm@ntu.edu.sg Science::Biological sciences::Molecular biology Unilamellar liposomes are sphere-shaped vesicles which are artificially created from natural phospholipids and/or cholesterol to mimic the composition of bilayer plasma membrane. Giant Unilamellar Vesicles (GUVs) are enclosed compartments or “microreactors” which offers a way to study the effect of confinement on cellular systems. Here we demonstrated several preparation approaches of GUVs, namely water-in-oil droplets with microfluidics apparatus, one-pot assembly water-in-oil droplets, GUVs with gentle hydration method and GUVs with reverse emulsion method. Specifically, we highlighted the advantages and challenges of each approach in reconstituting actin cytoskeleton, in an attempt to mimic the interaction between actin and actin-binding proteins – AtFH1, AtVLN4, XopR, Sac6 – in the cell-like vesicles. Moreover, we had also showed that we could vary the lipid compositions to create artificial cell of desired properties, as well as manipulating the proteins-lipids interaction on the membrane interface. Overall, we established capabilities of reconstituting actin polymerization inside an artificial cell-like confinement. Master of Science 2021-03-09T00:34:52Z 2021-03-09T00:34:52Z 2021 Thesis-Master by Research Susanti. (2021). Reconstitution of actin polymerization via artificial cells. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/146725 10.32657/10356/146725 en This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0). application/pdf Nanyang Technological University |
| spellingShingle | Science::Biological sciences::Molecular biology Susanti Reconstitution of actin polymerization via artificial cells |
| title | Reconstitution of actin polymerization via artificial cells |
| title_full | Reconstitution of actin polymerization via artificial cells |
| title_fullStr | Reconstitution of actin polymerization via artificial cells |
| title_full_unstemmed | Reconstitution of actin polymerization via artificial cells |
| title_short | Reconstitution of actin polymerization via artificial cells |
| title_sort | reconstitution of actin polymerization via artificial cells |
| topic | Science::Biological sciences::Molecular biology |
| url | https://hdl.handle.net/10356/146725 |
| work_keys_str_mv | AT susanti reconstitutionofactinpolymerizationviaartificialcells |