Comparative genomics of Leuconostoc carnosum
Leuconostoc carnosum is a known colonizer of meat-related food matrices. It reaches remarkably high loads during the shelf life in packaged meat products and plays a role in spoilage, although preservative effects have been proposed for some strains. In this study, the draft genomes of 17 strains of...
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| Format: | Journal Article |
| Language: | English |
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2021
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| Online Access: | https://hdl.handle.net/10356/146868 |
| _version_ | 1826119999335432192 |
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| author | Candeliere, Francesco Raimondi, Stefano Spampinato, Gloria Tay, Moon Yue Feng Amaretti, Alberto Schlundt, Joergen Rossi, Maddalena |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Candeliere, Francesco Raimondi, Stefano Spampinato, Gloria Tay, Moon Yue Feng Amaretti, Alberto Schlundt, Joergen Rossi, Maddalena |
| author_sort | Candeliere, Francesco |
| collection | NTU |
| description | Leuconostoc carnosum is a known colonizer of meat-related food matrices. It reaches remarkably high loads during the shelf life in packaged meat products and plays a role in spoilage, although preservative effects have been proposed for some strains. In this study, the draft genomes of 17 strains of L. carnosum (i.e., all the strains that have been sequenced so far) were compared to decipher their metabolic and functional potential and to determine their role in food transformations. Genome comparison and pathway reconstruction indicated that L. carnosum is a compact group of closely related heterofermentative bacteria sharing most of the metabolic features. Adaptation to a nitrogen-rich environment, such as meat, is evidenced by 23 peptidase genes identified in the core genome and by the autotrophy for nitrogen compounds including several amino acids, vitamins, and cofactors. Genes encoding the decarboxylases yielding biogenic amines were not present. All the strains harbored 1-4 of 32 different plasmids, bearing functions associated to proteins hydrolysis, transport of amino acids and oligopeptides, exopolysaccharides, and various resistances (e.g., to environmental stresses, bacteriophages, and heavy metals). Functions associated to bacteriocin synthesis, secretion, and immunity were also found in plasmids. While genes for lactococcin were found in most plasmids, only three harbored the genes for leucocin B, a class IIa antilisterial bacteriocin. Determinants of antibiotic resistances were absent in both plasmids and chromosomes. |
| first_indexed | 2024-10-01T05:09:21Z |
| format | Journal Article |
| id | ntu-10356/146868 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:09:21Z |
| publishDate | 2021 |
| record_format | dspace |
| spelling | ntu-10356/1468682023-12-29T06:48:52Z Comparative genomics of Leuconostoc carnosum Candeliere, Francesco Raimondi, Stefano Spampinato, Gloria Tay, Moon Yue Feng Amaretti, Alberto Schlundt, Joergen Rossi, Maddalena School of Chemical and Biomedical Engineering NTU Food Technology Centre Engineering::Bioengineering Leuconostoc Carnosum Genomics Leuconostoc carnosum is a known colonizer of meat-related food matrices. It reaches remarkably high loads during the shelf life in packaged meat products and plays a role in spoilage, although preservative effects have been proposed for some strains. In this study, the draft genomes of 17 strains of L. carnosum (i.e., all the strains that have been sequenced so far) were compared to decipher their metabolic and functional potential and to determine their role in food transformations. Genome comparison and pathway reconstruction indicated that L. carnosum is a compact group of closely related heterofermentative bacteria sharing most of the metabolic features. Adaptation to a nitrogen-rich environment, such as meat, is evidenced by 23 peptidase genes identified in the core genome and by the autotrophy for nitrogen compounds including several amino acids, vitamins, and cofactors. Genes encoding the decarboxylases yielding biogenic amines were not present. All the strains harbored 1-4 of 32 different plasmids, bearing functions associated to proteins hydrolysis, transport of amino acids and oligopeptides, exopolysaccharides, and various resistances (e.g., to environmental stresses, bacteriophages, and heavy metals). Functions associated to bacteriocin synthesis, secretion, and immunity were also found in plasmids. While genes for lactococcin were found in most plasmids, only three harbored the genes for leucocin B, a class IIa antilisterial bacteriocin. Determinants of antibiotic resistances were absent in both plasmids and chromosomes. Published version 2021-03-12T04:30:42Z 2021-03-12T04:30:42Z 2021 Journal Article Candeliere, F., Raimondi, S., Spampinato, G., Tay, M. Y. F., Amaretti, A., Schlundt, J. & Rossi, M. (2021). Comparative genomics of Leuconostoc carnosum. Frontiers in Microbiology, 11. https://dx.doi.org/10.3389/fmicb.2020.605127 1664-302X https://hdl.handle.net/10356/146868 10.3389/fmicb.2020.605127 33505375 2-s2.0-85099747601 11 en Frontiers in Microbiology © 2021 The Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. application/pdf |
| spellingShingle | Engineering::Bioengineering Leuconostoc Carnosum Genomics Candeliere, Francesco Raimondi, Stefano Spampinato, Gloria Tay, Moon Yue Feng Amaretti, Alberto Schlundt, Joergen Rossi, Maddalena Comparative genomics of Leuconostoc carnosum |
| title | Comparative genomics of Leuconostoc carnosum |
| title_full | Comparative genomics of Leuconostoc carnosum |
| title_fullStr | Comparative genomics of Leuconostoc carnosum |
| title_full_unstemmed | Comparative genomics of Leuconostoc carnosum |
| title_short | Comparative genomics of Leuconostoc carnosum |
| title_sort | comparative genomics of leuconostoc carnosum |
| topic | Engineering::Bioengineering Leuconostoc Carnosum Genomics |
| url | https://hdl.handle.net/10356/146868 |
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