Assessment of choroidal neovascularization perfusion : a pilot study with laser speckle flowgraphy
Purpose: The purpose of this study was to quantify perfusion in the area of choroidal neovascularization (CNV) using laser speckle flowgraphy (LSFG) before and after intravitreal anti-vascular endothelial growth factor (VEGF) injection. Methods: Retrospective case series. Fifteen eyes of 15 patients...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2021
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/147321 |
| _version_ | 1811678173603037184 |
|---|---|
| author | Calzetti, Giacomo Mora, Paolo Favilla, Stefania Ottonelli, Giorgia Devincenzi, Giulia Carta, Arturo Tedesco, Salvatore Mursch-Edlmayr, Anna Garhöfer, Gerhard Gandolfi, Stefano Schmetterer, Leopold |
| author2 | Lee Kong Chian School of Medicine (LKCMedicine) |
| author_facet | Lee Kong Chian School of Medicine (LKCMedicine) Calzetti, Giacomo Mora, Paolo Favilla, Stefania Ottonelli, Giorgia Devincenzi, Giulia Carta, Arturo Tedesco, Salvatore Mursch-Edlmayr, Anna Garhöfer, Gerhard Gandolfi, Stefano Schmetterer, Leopold |
| author_sort | Calzetti, Giacomo |
| collection | NTU |
| description | Purpose: The purpose of this study was to quantify perfusion in the area of choroidal neovascularization (CNV) using laser speckle flowgraphy (LSFG) before and after intravitreal anti-vascular endothelial growth factor (VEGF) injection. Methods: Retrospective case series. Fifteen eyes of 15 patients with treatment-naïve CNV due to age-related macular degeneration (AMD) and with available LSFG images were included. The main outcome was the mean blur rate (MBR) quantified as a measure of perfusion within the CNV area observed on indocyanine green angiography. Twelve patients had available longitudinal data until one month after the injection, used to evaluate changes in perfusion, central macular thickness (CMT), visual acuity, and ocular perfusion pressure. Reproducibility of LSFG measurements was investigated at each time point from two images taken within five minutes. Results: Intraclass correlation coefficients for LSFG measurements were higher than 0.8 indicating excellent reproducibility. There was a significant decrease in perfusion after one week (-26.4±14.4%;P=0.027), whereas, after one month, perfusion was no longer significantly different from baseline (P=0.121). CMT showed a progressive decrease over the follow-up period. Changes in perfusion were strongly correlated with changes in CMT after one week, but not thereafter. Conclusions: This pilot study suggests a method to select a region in the CNV area to quantify perfusion using LSFG. MBR could represent a parameter possibly related to regrowth of the CNV after anti-VEGF treatment. Large-scale studies are needed to assess the usefulness of LSFG in defining re-treatment criteria for neovascular AMD. |
| first_indexed | 2024-10-01T02:49:03Z |
| format | Journal Article |
| id | ntu-10356/147321 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:49:03Z |
| publishDate | 2021 |
| record_format | dspace |
| spelling | ntu-10356/1473212023-03-05T16:49:55Z Assessment of choroidal neovascularization perfusion : a pilot study with laser speckle flowgraphy Calzetti, Giacomo Mora, Paolo Favilla, Stefania Ottonelli, Giorgia Devincenzi, Giulia Carta, Arturo Tedesco, Salvatore Mursch-Edlmayr, Anna Garhöfer, Gerhard Gandolfi, Stefano Schmetterer, Leopold Lee Kong Chian School of Medicine (LKCMedicine) Science::Medicine Laser Speckle Flowgraphy Imaging Purpose: The purpose of this study was to quantify perfusion in the area of choroidal neovascularization (CNV) using laser speckle flowgraphy (LSFG) before and after intravitreal anti-vascular endothelial growth factor (VEGF) injection. Methods: Retrospective case series. Fifteen eyes of 15 patients with treatment-naïve CNV due to age-related macular degeneration (AMD) and with available LSFG images were included. The main outcome was the mean blur rate (MBR) quantified as a measure of perfusion within the CNV area observed on indocyanine green angiography. Twelve patients had available longitudinal data until one month after the injection, used to evaluate changes in perfusion, central macular thickness (CMT), visual acuity, and ocular perfusion pressure. Reproducibility of LSFG measurements was investigated at each time point from two images taken within five minutes. Results: Intraclass correlation coefficients for LSFG measurements were higher than 0.8 indicating excellent reproducibility. There was a significant decrease in perfusion after one week (-26.4±14.4%;P=0.027), whereas, after one month, perfusion was no longer significantly different from baseline (P=0.121). CMT showed a progressive decrease over the follow-up period. Changes in perfusion were strongly correlated with changes in CMT after one week, but not thereafter. Conclusions: This pilot study suggests a method to select a region in the CNV area to quantify perfusion using LSFG. MBR could represent a parameter possibly related to regrowth of the CNV after anti-VEGF treatment. Large-scale studies are needed to assess the usefulness of LSFG in defining re-treatment criteria for neovascular AMD. Published version 2021-03-31T03:50:53Z 2021-03-31T03:50:53Z 2020 Journal Article Calzetti, G., Mora, P., Favilla, S., Ottonelli, G., Devincenzi, G., Carta, A., Tedesco, S., Mursch-Edlmayr, A., Garhöfer, G., Gandolfi, S. & Schmetterer, L. (2020). Assessment of choroidal neovascularization perfusion : a pilot study with laser speckle flowgraphy. Translational Vision Science and Technology, 9(5). https://dx.doi.org/10.1167/tvst.9.5.9 2164-2591 https://hdl.handle.net/10356/147321 10.1167/tvst.9.5.9 32821481 2-s2.0-85083657636 5 9 en Translational Vision Science and Technology © 2020 The Author(s). This work is licensed under a Creative Commons Attribution-Non Commercial-No Derivatives 4.0 International License. application/pdf |
| spellingShingle | Science::Medicine Laser Speckle Flowgraphy Imaging Calzetti, Giacomo Mora, Paolo Favilla, Stefania Ottonelli, Giorgia Devincenzi, Giulia Carta, Arturo Tedesco, Salvatore Mursch-Edlmayr, Anna Garhöfer, Gerhard Gandolfi, Stefano Schmetterer, Leopold Assessment of choroidal neovascularization perfusion : a pilot study with laser speckle flowgraphy |
| title | Assessment of choroidal neovascularization perfusion : a pilot study with laser speckle flowgraphy |
| title_full | Assessment of choroidal neovascularization perfusion : a pilot study with laser speckle flowgraphy |
| title_fullStr | Assessment of choroidal neovascularization perfusion : a pilot study with laser speckle flowgraphy |
| title_full_unstemmed | Assessment of choroidal neovascularization perfusion : a pilot study with laser speckle flowgraphy |
| title_short | Assessment of choroidal neovascularization perfusion : a pilot study with laser speckle flowgraphy |
| title_sort | assessment of choroidal neovascularization perfusion a pilot study with laser speckle flowgraphy |
| topic | Science::Medicine Laser Speckle Flowgraphy Imaging |
| url | https://hdl.handle.net/10356/147321 |
| work_keys_str_mv | AT calzettigiacomo assessmentofchoroidalneovascularizationperfusionapilotstudywithlaserspeckleflowgraphy AT morapaolo assessmentofchoroidalneovascularizationperfusionapilotstudywithlaserspeckleflowgraphy AT favillastefania assessmentofchoroidalneovascularizationperfusionapilotstudywithlaserspeckleflowgraphy AT ottonelligiorgia assessmentofchoroidalneovascularizationperfusionapilotstudywithlaserspeckleflowgraphy AT devincenzigiulia assessmentofchoroidalneovascularizationperfusionapilotstudywithlaserspeckleflowgraphy AT cartaarturo assessmentofchoroidalneovascularizationperfusionapilotstudywithlaserspeckleflowgraphy AT tedescosalvatore assessmentofchoroidalneovascularizationperfusionapilotstudywithlaserspeckleflowgraphy AT murschedlmayranna assessmentofchoroidalneovascularizationperfusionapilotstudywithlaserspeckleflowgraphy AT garhofergerhard assessmentofchoroidalneovascularizationperfusionapilotstudywithlaserspeckleflowgraphy AT gandolfistefano assessmentofchoroidalneovascularizationperfusionapilotstudywithlaserspeckleflowgraphy AT schmettererleopold assessmentofchoroidalneovascularizationperfusionapilotstudywithlaserspeckleflowgraphy |