Catalytic properties of human apurinic/apyrimidinic endonuclease 1 (APE1) and development of biosensors of APE1 in living cells
Apurinic/apyrimidinic endonuclease 1 (APE1) is an essential enzyme involved in the DNA base excision repair pathway and responsible for restoring mutagenic and cytotoxic abasic lesions throughout cell cycles. APE1 will cleave the 5’ end phosphodiester backbone right next to the generated apurinic/ap...
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| 格式: | Thesis-Doctor of Philosophy |
| 语言: | English |
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Nanyang Technological University
2021
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| 在线阅读: | https://hdl.handle.net/10356/147410 |
| _version_ | 1826110969992970240 |
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| author | Wang, Tianxiang |
| author2 | Li Tianhu |
| author_facet | Li Tianhu Wang, Tianxiang |
| author_sort | Wang, Tianxiang |
| collection | NTU |
| description | Apurinic/apyrimidinic endonuclease 1 (APE1) is an essential enzyme involved in the DNA base excision repair pathway and responsible for restoring mutagenic and cytotoxic abasic lesions throughout cell cycles. APE1 will cleave the 5’ end phosphodiester backbone right next to the generated apurinic/apyrimidinic site (AP site) to produce a free 5’ phosphate termini linking to the AP site and a free 3’ hydroxyl termini on the regular nucleotide. APE1 is overexpressed in various types of cancer cells, proving that APE1 is a valid biomarker for cancer diagnosis and its inhibitors show potential in monotherapy and combination therapy for fighting cancers. In spite of its potential roles in cancer diagnosis, prognosis, and chemotherapy, however, various aspects of translational research about APE1 have not yet been carried out thus far. Herein, we have successfully developed graphene quantum dot-based biosensors for APE1 diagnosis both in cell-free systems and in living cells. Besides, investigation on diversity of substrates makes a great contribution to understand the catalytic properties of APE1 and further explore sequence-specific DNA as new APE1 inhibitors. |
| first_indexed | 2024-10-01T02:43:15Z |
| format | Thesis-Doctor of Philosophy |
| id | ntu-10356/147410 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:43:15Z |
| publishDate | 2021 |
| publisher | Nanyang Technological University |
| record_format | dspace |
| spelling | ntu-10356/1474102023-02-28T23:33:15Z Catalytic properties of human apurinic/apyrimidinic endonuclease 1 (APE1) and development of biosensors of APE1 in living cells Wang, Tianxiang Li Tianhu School of Physical and Mathematical Sciences THLI@ntu.edu.sg Science::Chemistry::Biochemistry Apurinic/apyrimidinic endonuclease 1 (APE1) is an essential enzyme involved in the DNA base excision repair pathway and responsible for restoring mutagenic and cytotoxic abasic lesions throughout cell cycles. APE1 will cleave the 5’ end phosphodiester backbone right next to the generated apurinic/apyrimidinic site (AP site) to produce a free 5’ phosphate termini linking to the AP site and a free 3’ hydroxyl termini on the regular nucleotide. APE1 is overexpressed in various types of cancer cells, proving that APE1 is a valid biomarker for cancer diagnosis and its inhibitors show potential in monotherapy and combination therapy for fighting cancers. In spite of its potential roles in cancer diagnosis, prognosis, and chemotherapy, however, various aspects of translational research about APE1 have not yet been carried out thus far. Herein, we have successfully developed graphene quantum dot-based biosensors for APE1 diagnosis both in cell-free systems and in living cells. Besides, investigation on diversity of substrates makes a great contribution to understand the catalytic properties of APE1 and further explore sequence-specific DNA as new APE1 inhibitors. Doctor of Philosophy 2021-04-01T12:39:40Z 2021-04-01T12:39:40Z 2021 Thesis-Doctor of Philosophy Wang, T. (2021). Catalytic properties of human apurinic/apyrimidinic endonuclease 1 (APE1) and development of biosensors of APE1 in living cells. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/147410 https://hdl.handle.net/10356/147410 10.32657/10356/147410 en This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0). application/pdf Nanyang Technological University |
| spellingShingle | Science::Chemistry::Biochemistry Wang, Tianxiang Catalytic properties of human apurinic/apyrimidinic endonuclease 1 (APE1) and development of biosensors of APE1 in living cells |
| title | Catalytic properties of human apurinic/apyrimidinic endonuclease 1 (APE1) and development of biosensors of APE1 in living cells |
| title_full | Catalytic properties of human apurinic/apyrimidinic endonuclease 1 (APE1) and development of biosensors of APE1 in living cells |
| title_fullStr | Catalytic properties of human apurinic/apyrimidinic endonuclease 1 (APE1) and development of biosensors of APE1 in living cells |
| title_full_unstemmed | Catalytic properties of human apurinic/apyrimidinic endonuclease 1 (APE1) and development of biosensors of APE1 in living cells |
| title_short | Catalytic properties of human apurinic/apyrimidinic endonuclease 1 (APE1) and development of biosensors of APE1 in living cells |
| title_sort | catalytic properties of human apurinic apyrimidinic endonuclease 1 ape1 and development of biosensors of ape1 in living cells |
| topic | Science::Chemistry::Biochemistry |
| url | https://hdl.handle.net/10356/147410 |
| work_keys_str_mv | AT wangtianxiang catalyticpropertiesofhumanapurinicapyrimidinicendonuclease1ape1anddevelopmentofbiosensorsofape1inlivingcells |