Pulmonary toxicity of nanoparticles in a lung-on-a-chip platform
The use of nanoparticles (NPs) started since last century. Due to their remarkable physical and chemical properties, NPs are used in a wide variety of applications, ranging from consumer products to biomedical applications. NPs are very small particles with sizes in the nano level. Due to the small...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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Nanyang Technological University
2021
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| Online Access: | https://hdl.handle.net/10356/147672 |
| _version_ | 1811678746808156160 |
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| author | Tan, Zou Wei |
| author2 | Dalton Tay Chor Yong |
| author_facet | Dalton Tay Chor Yong Tan, Zou Wei |
| author_sort | Tan, Zou Wei |
| collection | NTU |
| description | The use of nanoparticles (NPs) started since last century. Due to their remarkable physical and chemical properties, NPs are used in a wide variety of applications, ranging from consumer products to biomedical applications. NPs are very small particles with sizes in the nano level. Due to the small size of NPs, they can be easily inhaled by humans. The NPs can reach and be deposited in the alveolar regions of human lungs. Studies have shown that many of the common NPs used are toxic. When these NPs are deposited and accumulated in human lungs for a long period of time, it can bring about pulmonary health issues like asthma and lung cancer. It is therefore crucial to investigate the effects of toxicity of commonly used NPs to study their toxicity on human lung cells.
To test the toxicity of NPs, many studies use in vivo and in vitro models to determine nanotoxicity. However, both in vivo and in vitro models are not able to fully mimic a human lung microenvironment. A systematic assessment to study potential harmful effects of NPs on human lung cells is still lacking.
This study aimed to evaluate the toxicity of commonly used metal oxide NPs like CuO, TiO2 and ZnO NPs by characterizing and conducting in vitro cytotoxicity tests of these NPs on lung epithelial cells (A549). A 3D human lung-on-a-chip in vitro co-culture model which mimics the structure of functions the airway interface of human lung was adapted. This 3D lung-on-a-chip model was used to investigate the morphological changes and cell viability of A549 cells and human lung fibroblasts (HLF) upon exposure to these NPs. The various findings and observations will be presented in this report. |
| first_indexed | 2024-10-01T02:58:10Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/147672 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:58:10Z |
| publishDate | 2021 |
| publisher | Nanyang Technological University |
| record_format | dspace |
| spelling | ntu-10356/1476722023-03-04T15:44:28Z Pulmonary toxicity of nanoparticles in a lung-on-a-chip platform Tan, Zou Wei Dalton Tay Chor Yong School of Materials Science and Engineering cytay@ntu.edu.sg Engineering::Nanotechnology Engineering::Materials The use of nanoparticles (NPs) started since last century. Due to their remarkable physical and chemical properties, NPs are used in a wide variety of applications, ranging from consumer products to biomedical applications. NPs are very small particles with sizes in the nano level. Due to the small size of NPs, they can be easily inhaled by humans. The NPs can reach and be deposited in the alveolar regions of human lungs. Studies have shown that many of the common NPs used are toxic. When these NPs are deposited and accumulated in human lungs for a long period of time, it can bring about pulmonary health issues like asthma and lung cancer. It is therefore crucial to investigate the effects of toxicity of commonly used NPs to study their toxicity on human lung cells. To test the toxicity of NPs, many studies use in vivo and in vitro models to determine nanotoxicity. However, both in vivo and in vitro models are not able to fully mimic a human lung microenvironment. A systematic assessment to study potential harmful effects of NPs on human lung cells is still lacking. This study aimed to evaluate the toxicity of commonly used metal oxide NPs like CuO, TiO2 and ZnO NPs by characterizing and conducting in vitro cytotoxicity tests of these NPs on lung epithelial cells (A549). A 3D human lung-on-a-chip in vitro co-culture model which mimics the structure of functions the airway interface of human lung was adapted. This 3D lung-on-a-chip model was used to investigate the morphological changes and cell viability of A549 cells and human lung fibroblasts (HLF) upon exposure to these NPs. The various findings and observations will be presented in this report. Bachelor of Engineering (Materials Engineering) 2021-04-10T14:37:12Z 2021-04-10T14:37:12Z 2021 Final Year Project (FYP) Tan, Z. W. (2021). Pulmonary toxicity of nanoparticles in a lung-on-a-chip platform. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/147672 https://hdl.handle.net/10356/147672 en application/pdf Nanyang Technological University |
| spellingShingle | Engineering::Nanotechnology Engineering::Materials Tan, Zou Wei Pulmonary toxicity of nanoparticles in a lung-on-a-chip platform |
| title | Pulmonary toxicity of nanoparticles in a lung-on-a-chip platform |
| title_full | Pulmonary toxicity of nanoparticles in a lung-on-a-chip platform |
| title_fullStr | Pulmonary toxicity of nanoparticles in a lung-on-a-chip platform |
| title_full_unstemmed | Pulmonary toxicity of nanoparticles in a lung-on-a-chip platform |
| title_short | Pulmonary toxicity of nanoparticles in a lung-on-a-chip platform |
| title_sort | pulmonary toxicity of nanoparticles in a lung on a chip platform |
| topic | Engineering::Nanotechnology Engineering::Materials |
| url | https://hdl.handle.net/10356/147672 |
| work_keys_str_mv | AT tanzouwei pulmonarytoxicityofnanoparticlesinalungonachipplatform |