Synbiotic effect of L. fermentum PC1 and Spirulina on gut health and immunity
The modulation of the gut microbiome has been of great interest as the gut microbiome affects the host metabolism, and even establishing cross-talk with the immune system. Dietary interventions using probiotics, prebiotics and their combinations (synbiotics) have proven to be beneficial for human he...
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Final Year Project (FYP) |
| Language: | English |
| Published: |
Nanyang Technological University
2021
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/148544 |
| _version_ | 1826109754038026240 |
|---|---|
| author | Sim, Edric Shao Zhe |
| author2 | Ruedl Christiane |
| author_facet | Ruedl Christiane Sim, Edric Shao Zhe |
| author_sort | Sim, Edric Shao Zhe |
| collection | NTU |
| description | The modulation of the gut microbiome has been of great interest as the gut microbiome affects the host metabolism, and even establishing cross-talk with the immune system. Dietary interventions using probiotics, prebiotics and their combinations (synbiotics) have proven to be beneficial for human health. However, the mechanisms of synbiotics on the gut microbiome and immunity remains poorly characterized. Using an ex-vivo gut model supplemented with fecal samples, we hereby report that the combination of Lactobacillus fermentum PC1 and Spirulina as a synbiotic modulates the gut microbiome by promoting lactobacilli growth, exhibiting anti-microbial activity on enteric bacteria and enhancing acetic acid production. Using an in-vitro FMS-like tyrosine kinase 3 ligand (FLT3L)-stimulated bone-marrow dendritic cells (BMDCs), we also report that synbiotic differently upregulates dendritic cells (DCs) maturation and induce pro-inflammatory TNF-α, IL-6, IL-1β cytokines. Interestingly, synergism was only observed in the production of IL-1β cytokine. This might represent an alternative mechanism for synbiotic effects, other than the microbiota-driven modulation of immunity. Together, these findings successfully characterize synbiotic effects on gut health and immunity. However, the functionality of this synbiotic should be studied further in various in-vitro and in-vivo models, potentially paving way for precision application to individuals in clinical settings. |
| first_indexed | 2024-10-01T02:23:08Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/148544 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:23:08Z |
| publishDate | 2021 |
| publisher | Nanyang Technological University |
| record_format | dspace |
| spelling | ntu-10356/1485442023-02-28T18:08:18Z Synbiotic effect of L. fermentum PC1 and Spirulina on gut health and immunity Sim, Edric Shao Zhe Ruedl Christiane School of Biological Sciences Patricia Conway Ruedl@ntu.edu.sg Science::Biological sciences The modulation of the gut microbiome has been of great interest as the gut microbiome affects the host metabolism, and even establishing cross-talk with the immune system. Dietary interventions using probiotics, prebiotics and their combinations (synbiotics) have proven to be beneficial for human health. However, the mechanisms of synbiotics on the gut microbiome and immunity remains poorly characterized. Using an ex-vivo gut model supplemented with fecal samples, we hereby report that the combination of Lactobacillus fermentum PC1 and Spirulina as a synbiotic modulates the gut microbiome by promoting lactobacilli growth, exhibiting anti-microbial activity on enteric bacteria and enhancing acetic acid production. Using an in-vitro FMS-like tyrosine kinase 3 ligand (FLT3L)-stimulated bone-marrow dendritic cells (BMDCs), we also report that synbiotic differently upregulates dendritic cells (DCs) maturation and induce pro-inflammatory TNF-α, IL-6, IL-1β cytokines. Interestingly, synergism was only observed in the production of IL-1β cytokine. This might represent an alternative mechanism for synbiotic effects, other than the microbiota-driven modulation of immunity. Together, these findings successfully characterize synbiotic effects on gut health and immunity. However, the functionality of this synbiotic should be studied further in various in-vitro and in-vivo models, potentially paving way for precision application to individuals in clinical settings. Bachelor of Science in Biological Sciences 2021-05-05T08:43:54Z 2021-05-05T08:43:54Z 2021 Final Year Project (FYP) Sim, E. S. Z. (2021). Synbiotic effect of L. fermentum PC1 and Spirulina on gut health and immunity. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/148544 https://hdl.handle.net/10356/148544 en application/pdf Nanyang Technological University |
| spellingShingle | Science::Biological sciences Sim, Edric Shao Zhe Synbiotic effect of L. fermentum PC1 and Spirulina on gut health and immunity |
| title | Synbiotic effect of L. fermentum PC1 and Spirulina on gut health and immunity |
| title_full | Synbiotic effect of L. fermentum PC1 and Spirulina on gut health and immunity |
| title_fullStr | Synbiotic effect of L. fermentum PC1 and Spirulina on gut health and immunity |
| title_full_unstemmed | Synbiotic effect of L. fermentum PC1 and Spirulina on gut health and immunity |
| title_short | Synbiotic effect of L. fermentum PC1 and Spirulina on gut health and immunity |
| title_sort | synbiotic effect of l fermentum pc1 and spirulina on gut health and immunity |
| topic | Science::Biological sciences |
| url | https://hdl.handle.net/10356/148544 |
| work_keys_str_mv | AT simedricshaozhe synbioticeffectoflfermentumpc1andspirulinaonguthealthandimmunity |