Enhanced sample pre-concentration by ion concentration polarization on a paraffin coated converging microfluidic paper based analytical platform
Microfluidic paper-based analytical devices (μPADs) represent a modest and feasible alternative for conventional analytical methods. However, the inadequate sensitivity of these devices limits the possible applications of μPADs. In this scenario, inducing ion concentration polarization (ICP) on μPAD...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2021
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| Online Access: | https://hdl.handle.net/10356/148757 |
| _version_ | 1811682824752726016 |
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| author | Perera, A. T. K. Phan, Dinh-Tuan Pudasaini, Sanam Liu, Yu Yang, Chun |
| author2 | School of Mechanical and Aerospace Engineering |
| author_facet | School of Mechanical and Aerospace Engineering Perera, A. T. K. Phan, Dinh-Tuan Pudasaini, Sanam Liu, Yu Yang, Chun |
| author_sort | Perera, A. T. K. |
| collection | NTU |
| description | Microfluidic paper-based analytical devices (μPADs) represent a modest and feasible alternative for conventional analytical methods. However, the inadequate sensitivity of these devices limits the possible applications of μPADs. In this scenario, inducing ion concentration polarization (ICP) on μPADs has shown promise to overcome this limitation by preconcentrating the analytes of interest. Here, we report a μPAD implementing ICP using an off-shelf Nafion® membrane as the perm selective membrane. Two types of devices with a geometrical configuration of a straight channel converging at the middle connecting to circular reservoirs at the end of channels were fabricated. The devices are comprised of a single input channel and an absorption channel. The Nafion membrane is attached to the absorption channel of the device, which is encased by heating with paraffin films at both sides to lower the electro-osmotic flow generated by an applied DC electric field that is needed for ICP. The field induced ICP enables obtaining a maximum concentration factor of more than 2000 folds for fluorescein sodium salt solution on the μPAD. Also, since evaporation of the sample solution was reported to be of great influence on the concentration factor, we analyze the effect of sample solution evaporation on sample preconcentration. Furthermore, our reported fabrication method for μPAD can lower the fabrication cost down to 0.3 USD. This device shows the potential to be developed for serving as a diagnostic and environmental monitoring platform. |
| first_indexed | 2024-10-01T04:02:59Z |
| format | Journal Article |
| id | ntu-10356/148757 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:02:59Z |
| publishDate | 2021 |
| record_format | dspace |
| spelling | ntu-10356/1487572021-05-17T08:05:23Z Enhanced sample pre-concentration by ion concentration polarization on a paraffin coated converging microfluidic paper based analytical platform Perera, A. T. K. Phan, Dinh-Tuan Pudasaini, Sanam Liu, Yu Yang, Chun School of Mechanical and Aerospace Engineering Interdisciplinary Graduate School (IGS) School of Civil and Environmental Engineering Engineering::Mechanical engineering Analytic Equipment Electric Fields Microfluidic paper-based analytical devices (μPADs) represent a modest and feasible alternative for conventional analytical methods. However, the inadequate sensitivity of these devices limits the possible applications of μPADs. In this scenario, inducing ion concentration polarization (ICP) on μPADs has shown promise to overcome this limitation by preconcentrating the analytes of interest. Here, we report a μPAD implementing ICP using an off-shelf Nafion® membrane as the perm selective membrane. Two types of devices with a geometrical configuration of a straight channel converging at the middle connecting to circular reservoirs at the end of channels were fabricated. The devices are comprised of a single input channel and an absorption channel. The Nafion membrane is attached to the absorption channel of the device, which is encased by heating with paraffin films at both sides to lower the electro-osmotic flow generated by an applied DC electric field that is needed for ICP. The field induced ICP enables obtaining a maximum concentration factor of more than 2000 folds for fluorescein sodium salt solution on the μPAD. Also, since evaporation of the sample solution was reported to be of great influence on the concentration factor, we analyze the effect of sample solution evaporation on sample preconcentration. Furthermore, our reported fabrication method for μPAD can lower the fabrication cost down to 0.3 USD. This device shows the potential to be developed for serving as a diagnostic and environmental monitoring platform. Ministry of Education (MOE) Nanyang Technological University Published version The authors acknowledge financial support from the Interdisciplinary Graduate Programme and Nanyang Environmental and Water Research Institute, Nanyang Technological University, Singapore and the Ministry of Education of Singapore under Tier 1 Grant No. RG83/15. 2021-05-17T08:05:23Z 2021-05-17T08:05:23Z 2020 Journal Article Perera, A. T. K., Phan, D., Pudasaini, S., Liu, Y. & Yang, C. (2020). Enhanced sample pre-concentration by ion concentration polarization on a paraffin coated converging microfluidic paper based analytical platform. Biomicrofluidics, 14(1). https://dx.doi.org/10.1063/1.5133946 1932-1058 0000-0002-5730-8167 0000-0001-7060-0173 0000-0001-9782-3369 0000-0003-1191-7642 https://hdl.handle.net/10356/148757 10.1063/1.5133946 31933713 2-s2.0-85077516602 1 14 en RG83/15 Biomicrofluidics © 2020 The Author(s). All rights reserved. This paper was published by American Institute of Physics (AIP) in Applied Physics Reviews and is made available with permission of The Author(s). application/pdf |
| spellingShingle | Engineering::Mechanical engineering Analytic Equipment Electric Fields Perera, A. T. K. Phan, Dinh-Tuan Pudasaini, Sanam Liu, Yu Yang, Chun Enhanced sample pre-concentration by ion concentration polarization on a paraffin coated converging microfluidic paper based analytical platform |
| title | Enhanced sample pre-concentration by ion concentration polarization on a paraffin coated converging microfluidic paper based analytical platform |
| title_full | Enhanced sample pre-concentration by ion concentration polarization on a paraffin coated converging microfluidic paper based analytical platform |
| title_fullStr | Enhanced sample pre-concentration by ion concentration polarization on a paraffin coated converging microfluidic paper based analytical platform |
| title_full_unstemmed | Enhanced sample pre-concentration by ion concentration polarization on a paraffin coated converging microfluidic paper based analytical platform |
| title_short | Enhanced sample pre-concentration by ion concentration polarization on a paraffin coated converging microfluidic paper based analytical platform |
| title_sort | enhanced sample pre concentration by ion concentration polarization on a paraffin coated converging microfluidic paper based analytical platform |
| topic | Engineering::Mechanical engineering Analytic Equipment Electric Fields |
| url | https://hdl.handle.net/10356/148757 |
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