A surrogate of Roux-en-Y gastric bypass (the enterogastro anastomosis surgery) regulates multiple beta-cell pathways during resolution of diabetes in ob/ob mice
Background: Bariatric surgery is an effective treatment for type 2 diabetes. Early post-surgical enhancement of insulin secretion is key for diabetes remission. The full complement of mechanisms responsible for improved pancreatic beta cell functionality after bariatric surgery is still unclear. Our...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2021
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| Online Access: | https://hdl.handle.net/10356/148805 |
| _version_ | 1826125280208486400 |
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| author | Amouyal, Chloé Castel, Julien Guay, Claudiane Lacombe, Amélie Denom, Jessica Migrenne-Li, Stéphanie Rouault, Christine Marquet, Florian Georgiadou, Eleni Stylianides, Theodoros Luquet, Serge Le Stunff, Hervé Scharfmann, Raphael Clément, Karine Rutter, Guy A. Taboureau, Olivier Magnan, Christophe Regazzi, Romano Andreelli, Fabrizio |
| author2 | Lee Kong Chian School of Medicine (LKCMedicine) |
| author_facet | Lee Kong Chian School of Medicine (LKCMedicine) Amouyal, Chloé Castel, Julien Guay, Claudiane Lacombe, Amélie Denom, Jessica Migrenne-Li, Stéphanie Rouault, Christine Marquet, Florian Georgiadou, Eleni Stylianides, Theodoros Luquet, Serge Le Stunff, Hervé Scharfmann, Raphael Clément, Karine Rutter, Guy A. Taboureau, Olivier Magnan, Christophe Regazzi, Romano Andreelli, Fabrizio |
| author_sort | Amouyal, Chloé |
| collection | NTU |
| description | Background: Bariatric surgery is an effective treatment for type 2 diabetes. Early post-surgical enhancement of insulin secretion is key for diabetes remission. The full complement of mechanisms responsible for improved pancreatic beta cell functionality after bariatric surgery is still unclear. Our aim was to identify pathways, evident in the islet transcriptome, that characterize the adaptive response to bariatric surgery independently of body weight changes. Methods: We performed entero-gastro-anastomosis (EGA) with pyloric ligature in leptin-deficient ob/ob mice as a surrogate of Roux-en-Y gastric bypass (RYGB) in humans. Multiple approaches such as determination of glucose tolerance, GLP-1 and insulin secretion, whole body insulin sensitivity, ex vivo glucose-stimulated insulin secretion (GSIS) and functional multicellular Ca2+-imaging, profiling of mRNA and of miRNA expression were utilized to identify significant biological processes involved in pancreatic islet recovery. |
| first_indexed | 2024-10-01T06:34:04Z |
| format | Journal Article |
| id | ntu-10356/148805 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:34:04Z |
| publishDate | 2021 |
| record_format | dspace |
| spelling | ntu-10356/1488052023-03-05T16:45:32Z A surrogate of Roux-en-Y gastric bypass (the enterogastro anastomosis surgery) regulates multiple beta-cell pathways during resolution of diabetes in ob/ob mice Amouyal, Chloé Castel, Julien Guay, Claudiane Lacombe, Amélie Denom, Jessica Migrenne-Li, Stéphanie Rouault, Christine Marquet, Florian Georgiadou, Eleni Stylianides, Theodoros Luquet, Serge Le Stunff, Hervé Scharfmann, Raphael Clément, Karine Rutter, Guy A. Taboureau, Olivier Magnan, Christophe Regazzi, Romano Andreelli, Fabrizio Lee Kong Chian School of Medicine (LKCMedicine) Science::Medicine Diabetes Bariatric Surgery Background: Bariatric surgery is an effective treatment for type 2 diabetes. Early post-surgical enhancement of insulin secretion is key for diabetes remission. The full complement of mechanisms responsible for improved pancreatic beta cell functionality after bariatric surgery is still unclear. Our aim was to identify pathways, evident in the islet transcriptome, that characterize the adaptive response to bariatric surgery independently of body weight changes. Methods: We performed entero-gastro-anastomosis (EGA) with pyloric ligature in leptin-deficient ob/ob mice as a surrogate of Roux-en-Y gastric bypass (RYGB) in humans. Multiple approaches such as determination of glucose tolerance, GLP-1 and insulin secretion, whole body insulin sensitivity, ex vivo glucose-stimulated insulin secretion (GSIS) and functional multicellular Ca2+-imaging, profiling of mRNA and of miRNA expression were utilized to identify significant biological processes involved in pancreatic islet recovery. Published version This project has received funding from the European Union’s Horizon 2020 research and innovation programme via the Innovative Medicines Initiative 2 Joint Undertaking under grant agreement No 115881 (RHAPSODY) to G.A.R., C.M. and R.S. This Joint Undertaking receives support from the European Union’s Horizon 2020 research and innovation programme and EFPIA. C.A. received grants for this work by INSERM (poste d’accueil INSERM), Societ e Francophone du Diabete and Institut Benjamin Delessert. G.A.R. was supported by a Wellcome Trust Investigator Award (212625/Z/18/Z), MRC Programme grants (MR/R022259/1, MR/J0003042/1, MR/L020149/1) and by Diabetes UK (BDA/11/0004210, BDA/15/0005275, BDA 16/ 0005485) project grants. RR was supported by National Science Foundation (310030 188447). F.A. received a grant by Fondation de l’Avenir for this work. Funders had no role in study design, data collection, data analysis, interpretation, writing of the report. 2021-06-08T04:09:42Z 2021-06-08T04:09:42Z 2020 Journal Article Amouyal, C., Castel, J., Guay, C., Lacombe, A., Denom, J., Migrenne-Li, S., Rouault, C., Marquet, F., Georgiadou, E., Stylianides, T., Luquet, S., Le Stunff, H., Scharfmann, R., Clément, K., Rutter, G. A., Taboureau, O., Magnan, C., Regazzi, R. & Andreelli, F. (2020). A surrogate of Roux-en-Y gastric bypass (the enterogastro anastomosis surgery) regulates multiple beta-cell pathways during resolution of diabetes in ob/ob mice. EBioMedicine, 58, 102895-. https://dx.doi.org/10.1016/j.ebiom.2020.102895 2352-3964 https://hdl.handle.net/10356/148805 10.1016/j.ebiom.2020.102895 32739864 2-s2.0-85088792679 58 102895 en EBioMedicine © 2020 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license. (http://creativecommons.org/licenses/by-nc-nd/4.0/) application/pdf |
| spellingShingle | Science::Medicine Diabetes Bariatric Surgery Amouyal, Chloé Castel, Julien Guay, Claudiane Lacombe, Amélie Denom, Jessica Migrenne-Li, Stéphanie Rouault, Christine Marquet, Florian Georgiadou, Eleni Stylianides, Theodoros Luquet, Serge Le Stunff, Hervé Scharfmann, Raphael Clément, Karine Rutter, Guy A. Taboureau, Olivier Magnan, Christophe Regazzi, Romano Andreelli, Fabrizio A surrogate of Roux-en-Y gastric bypass (the enterogastro anastomosis surgery) regulates multiple beta-cell pathways during resolution of diabetes in ob/ob mice |
| title | A surrogate of Roux-en-Y gastric bypass (the enterogastro anastomosis surgery) regulates multiple beta-cell pathways during resolution of diabetes in ob/ob mice |
| title_full | A surrogate of Roux-en-Y gastric bypass (the enterogastro anastomosis surgery) regulates multiple beta-cell pathways during resolution of diabetes in ob/ob mice |
| title_fullStr | A surrogate of Roux-en-Y gastric bypass (the enterogastro anastomosis surgery) regulates multiple beta-cell pathways during resolution of diabetes in ob/ob mice |
| title_full_unstemmed | A surrogate of Roux-en-Y gastric bypass (the enterogastro anastomosis surgery) regulates multiple beta-cell pathways during resolution of diabetes in ob/ob mice |
| title_short | A surrogate of Roux-en-Y gastric bypass (the enterogastro anastomosis surgery) regulates multiple beta-cell pathways during resolution of diabetes in ob/ob mice |
| title_sort | surrogate of roux en y gastric bypass the enterogastro anastomosis surgery regulates multiple beta cell pathways during resolution of diabetes in ob ob mice |
| topic | Science::Medicine Diabetes Bariatric Surgery |
| url | https://hdl.handle.net/10356/148805 |
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