Characterisation of proteinaceous material in selected food processing wastewater
One of the many resources that can be valorised from food processing wastewater (FPWW) is proteinaceous material. However, the incredibly broad array and complex nature of proteinaceous material that is present in the aliquot have made it difficult to characterize and determine the specific componen...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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Nanyang Technological University
2021
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| Online Access: | https://hdl.handle.net/10356/149131 |
| _version_ | 1811678235467972608 |
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| author | Tan, Ee Tang |
| author2 | Zhou Yan |
| author_facet | Zhou Yan Tan, Ee Tang |
| author_sort | Tan, Ee Tang |
| collection | NTU |
| description | One of the many resources that can be valorised from food processing wastewater (FPWW) is proteinaceous material. However, the incredibly broad array and complex nature of proteinaceous material that is present in the aliquot have made it difficult to characterize and determine the specific components. In this study, the characterisation of the proteinaceous material in the FPWW collected from Mr Bean and the Asia Pacific Breweries through using both basic analytical techniques and advanced spectroscopic methods are addressed. Various analytical strategies, such as ‘total nitrogen analyser (TN)’, ‘carbon, hydrogen, nitrogen and sulphur analyser (CHNS)’, ‘liquid chromatography – organic carbon detection – organic nitrogen detection (LC-OCD-OND)’, ‘liquid chromatography – mass spectrometry (LCMS)’ and ‘colorimetric protein assay kits’, that would be suitable for determining the concentration of proteinaceous matter in wastewater samples have been compared and evaluated. Results have proven that analytical methods such as TN, CHNS and LC-OCD-OND are inconclusive to be a good representation of proteinaceous concentration in the wastewater samples. Using the interclass correlation coefficient, a comparison of eleven protein assay methods with 24 different heterogenous proteinaceous samples shown that the ‘Micro BCA with Protein Precipitation’ method proceeds excellent reliability. It was then used to estimate the concentration of the protein in the wastewater and compared with amino acid analysis using LCMS, although the latter method is a better analytical method in both characterising and quantifying the proteinaceous material in the FPWW. |
| first_indexed | 2024-10-01T02:50:02Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/149131 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:50:02Z |
| publishDate | 2021 |
| publisher | Nanyang Technological University |
| record_format | dspace |
| spelling | ntu-10356/1491312021-05-12T01:11:27Z Characterisation of proteinaceous material in selected food processing wastewater Tan, Ee Tang Zhou Yan School of Civil and Environmental Engineering Advanced Environmental Biotechnology Centre (AEBC) ZhouYan@ntu.edu.sg Engineering::Environmental engineering::Water treatment One of the many resources that can be valorised from food processing wastewater (FPWW) is proteinaceous material. However, the incredibly broad array and complex nature of proteinaceous material that is present in the aliquot have made it difficult to characterize and determine the specific components. In this study, the characterisation of the proteinaceous material in the FPWW collected from Mr Bean and the Asia Pacific Breweries through using both basic analytical techniques and advanced spectroscopic methods are addressed. Various analytical strategies, such as ‘total nitrogen analyser (TN)’, ‘carbon, hydrogen, nitrogen and sulphur analyser (CHNS)’, ‘liquid chromatography – organic carbon detection – organic nitrogen detection (LC-OCD-OND)’, ‘liquid chromatography – mass spectrometry (LCMS)’ and ‘colorimetric protein assay kits’, that would be suitable for determining the concentration of proteinaceous matter in wastewater samples have been compared and evaluated. Results have proven that analytical methods such as TN, CHNS and LC-OCD-OND are inconclusive to be a good representation of proteinaceous concentration in the wastewater samples. Using the interclass correlation coefficient, a comparison of eleven protein assay methods with 24 different heterogenous proteinaceous samples shown that the ‘Micro BCA with Protein Precipitation’ method proceeds excellent reliability. It was then used to estimate the concentration of the protein in the wastewater and compared with amino acid analysis using LCMS, although the latter method is a better analytical method in both characterising and quantifying the proteinaceous material in the FPWW. Bachelor of Engineering (Environmental Engineering) 2021-05-12T01:11:27Z 2021-05-12T01:11:27Z 2021 Final Year Project (FYP) Tan, E. T. (2021). Characterisation of proteinaceous material in selected food processing wastewater. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/149131 https://hdl.handle.net/10356/149131 en application/pdf Nanyang Technological University |
| spellingShingle | Engineering::Environmental engineering::Water treatment Tan, Ee Tang Characterisation of proteinaceous material in selected food processing wastewater |
| title | Characterisation of proteinaceous material in selected food processing wastewater |
| title_full | Characterisation of proteinaceous material in selected food processing wastewater |
| title_fullStr | Characterisation of proteinaceous material in selected food processing wastewater |
| title_full_unstemmed | Characterisation of proteinaceous material in selected food processing wastewater |
| title_short | Characterisation of proteinaceous material in selected food processing wastewater |
| title_sort | characterisation of proteinaceous material in selected food processing wastewater |
| topic | Engineering::Environmental engineering::Water treatment |
| url | https://hdl.handle.net/10356/149131 |
| work_keys_str_mv | AT taneetang characterisationofproteinaceousmaterialinselectedfoodprocessingwastewater |