Study on effect of surfactant concentration of esophageal scaffold
Objective: The author has conducted series of experiments to investigate the optimal rate of decellularization on porcine esophagi using varied surfactant concentrations with expected retention of folded internal structure and extracellular matrix (ECM) scaffold. Methods: Porcine esophagi, collected...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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Nanyang Technological University
2021
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| Online Access: | https://hdl.handle.net/10356/149160 |
| _version_ | 1811685089693663232 |
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| author | Nur Khalisah Noorman |
| author2 | Chian Kerm Sin |
| author_facet | Chian Kerm Sin Nur Khalisah Noorman |
| author_sort | Nur Khalisah Noorman |
| collection | NTU |
| description | Objective: The author has conducted series of experiments to investigate the optimal rate of decellularization on porcine esophagi using varied surfactant concentrations with expected retention of folded internal structure and extracellular matrix (ECM) scaffold. Methods: Porcine esophagi, collected from a local abattoir, were treated and decellularized with Sodium Dodecyl Sulphate (SDS) in varying concentrations of 0.2%, 0.5%, 0.8%, 1% and 2% w/v SDS. After decellularization, results were characterized by: 1) visual cross-sectional assessment, 2) DNA quantification and 3) histology study. Results: Esophagus treated with the lowest SDS concentration is not able to reach complete decellularization due to presence of cell remnants in the ECM. On the contrary, esophagus treated in higher SDS concentrations concluded to reach complete decellularization however lost its folded mucosa. An optimum SDS concentration range within 0.5% to 0.8% w/v SDS is determined to be carried out for further studies. Conclusion: Increasing SDS concentration improves decellularization however it is limited to the optimum range. Beyond the stipulated range, it may have adverse effects on the folded internal structure and ECM scaffold. |
| first_indexed | 2024-10-01T04:38:59Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/149160 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:38:59Z |
| publishDate | 2021 |
| publisher | Nanyang Technological University |
| record_format | dspace |
| spelling | ntu-10356/1491602021-05-14T07:07:55Z Study on effect of surfactant concentration of esophageal scaffold Nur Khalisah Noorman Chian Kerm Sin School of Mechanical and Aerospace Engineering ASKSChian@ntu.edu.sg Engineering::Mechanical engineering Objective: The author has conducted series of experiments to investigate the optimal rate of decellularization on porcine esophagi using varied surfactant concentrations with expected retention of folded internal structure and extracellular matrix (ECM) scaffold. Methods: Porcine esophagi, collected from a local abattoir, were treated and decellularized with Sodium Dodecyl Sulphate (SDS) in varying concentrations of 0.2%, 0.5%, 0.8%, 1% and 2% w/v SDS. After decellularization, results were characterized by: 1) visual cross-sectional assessment, 2) DNA quantification and 3) histology study. Results: Esophagus treated with the lowest SDS concentration is not able to reach complete decellularization due to presence of cell remnants in the ECM. On the contrary, esophagus treated in higher SDS concentrations concluded to reach complete decellularization however lost its folded mucosa. An optimum SDS concentration range within 0.5% to 0.8% w/v SDS is determined to be carried out for further studies. Conclusion: Increasing SDS concentration improves decellularization however it is limited to the optimum range. Beyond the stipulated range, it may have adverse effects on the folded internal structure and ECM scaffold. Bachelor of Engineering (Mechanical Engineering) 2021-05-14T07:07:55Z 2021-05-14T07:07:55Z 2021 Final Year Project (FYP) Nur Khalisah Noorman (2021). Study on effect of surfactant concentration of esophageal scaffold. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/149160 https://hdl.handle.net/10356/149160 en B362 application/pdf Nanyang Technological University |
| spellingShingle | Engineering::Mechanical engineering Nur Khalisah Noorman Study on effect of surfactant concentration of esophageal scaffold |
| title | Study on effect of surfactant concentration of esophageal scaffold |
| title_full | Study on effect of surfactant concentration of esophageal scaffold |
| title_fullStr | Study on effect of surfactant concentration of esophageal scaffold |
| title_full_unstemmed | Study on effect of surfactant concentration of esophageal scaffold |
| title_short | Study on effect of surfactant concentration of esophageal scaffold |
| title_sort | study on effect of surfactant concentration of esophageal scaffold |
| topic | Engineering::Mechanical engineering |
| url | https://hdl.handle.net/10356/149160 |
| work_keys_str_mv | AT nurkhalisahnoorman studyoneffectofsurfactantconcentrationofesophagealscaffold |