Contrast of nuclei in stratified squamous epithelium in optical coherence tomography images at 800 nm
Imaging nuclei of keratinocytes in the stratified squamous epithelium has been a subject of intense research since nucleus associated cellular atypia is the key criteria for the screening and diagnosis of epithelial cancers and their precursors. However, keratinocyte nuclei have been reported to be...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2021
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/150186 |
| _version_ | 1826117293164199936 |
|---|---|
| author | Chen, Si Liu, Xinyu Wang, Nanshuo Ding, Qianshan Wang, Xianghong Ge, Xin Bo, En Yu, Xiaojun Yu, Honggang Xu, Chenjie Liu, Linbo |
| author2 | School of Electrical and Electronic Engineering |
| author_facet | School of Electrical and Electronic Engineering Chen, Si Liu, Xinyu Wang, Nanshuo Ding, Qianshan Wang, Xianghong Ge, Xin Bo, En Yu, Xiaojun Yu, Honggang Xu, Chenjie Liu, Linbo |
| author_sort | Chen, Si |
| collection | NTU |
| description | Imaging nuclei of keratinocytes in the stratified squamous epithelium has been a subject of intense research since nucleus associated cellular atypia is the key criteria for the screening and diagnosis of epithelial cancers and their precursors. However, keratinocyte nuclei have been reported to be either low scattering or high scattering, so that these inconsistent reports might have led to misinterpretations of optical images, and more importantly, hindered the establishment of optical diagnostic criteria. We disclose that they are generally low scattering in the core using Micro‐optical coherence tomography (μOCT) of 1.28‐μm axial resolution in vivo; those previously reported “high scattering” or “bright” signals from nuclei are likely from the nucleocytoplasmic boundary, and the low‐scattering nuclear cores were missed possibly due to insufficient axial resolutions (~4μm). It is further demonstrated that the high scattering signals may be associated with flattening of nuclei and cytoplasmic glycogen accumulation, which are valuable cytologic hallmarks of cell maturation. |
| first_indexed | 2024-10-01T04:25:15Z |
| format | Journal Article |
| id | ntu-10356/150186 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:25:15Z |
| publishDate | 2021 |
| record_format | dspace |
| spelling | ntu-10356/1501862021-05-20T01:13:42Z Contrast of nuclei in stratified squamous epithelium in optical coherence tomography images at 800 nm Chen, Si Liu, Xinyu Wang, Nanshuo Ding, Qianshan Wang, Xianghong Ge, Xin Bo, En Yu, Xiaojun Yu, Honggang Xu, Chenjie Liu, Linbo School of Electrical and Electronic Engineering School of Chemical and Biomedical Engineering Engineering::Bioengineering Epithelial Cancer Nucleus Imaging nuclei of keratinocytes in the stratified squamous epithelium has been a subject of intense research since nucleus associated cellular atypia is the key criteria for the screening and diagnosis of epithelial cancers and their precursors. However, keratinocyte nuclei have been reported to be either low scattering or high scattering, so that these inconsistent reports might have led to misinterpretations of optical images, and more importantly, hindered the establishment of optical diagnostic criteria. We disclose that they are generally low scattering in the core using Micro‐optical coherence tomography (μOCT) of 1.28‐μm axial resolution in vivo; those previously reported “high scattering” or “bright” signals from nuclei are likely from the nucleocytoplasmic boundary, and the low‐scattering nuclear cores were missed possibly due to insufficient axial resolutions (~4μm). It is further demonstrated that the high scattering signals may be associated with flattening of nuclei and cytoplasmic glycogen accumulation, which are valuable cytologic hallmarks of cell maturation. Agency for Science, Technology and Research (A*STAR) Ministry of Education (MOE) Nanyang Technological University National Medical Research Council (NMRC) National Research Foundation (NRF) Accepted version This research is supported by the Singapore Ministry ofHealth's National Medical Research Council under its Coop-erative Basic Research Grant (NMRC/CBRG/0036/2013),National Research Foundation Singapore under its Competi-tive Research Program (NRF-CRP13-2014-05), Ministry ofEducation Singapore under its Academic Research FundTier 1 (2018-T1-001-144), Agency for Science, Technologyand Research (A*STAR) under its Industrial AlignmentFund (Pre-positioning) (H17/01/a0/008), and NTU-AIT-MUV program in advanced biomedical imaging (NAM/15005). 2021-05-20T01:13:42Z 2021-05-20T01:13:42Z 2019 Journal Article Chen, S., Liu, X., Wang, N., Ding, Q., Wang, X., Ge, X., Bo, E., Yu, X., Yu, H., Xu, C. & Liu, L. (2019). Contrast of nuclei in stratified squamous epithelium in optical coherence tomography images at 800 nm. Journal of Biophotonics, 12(9), e201900073-. https://dx.doi.org/10.1002/jbio.201900073 1864-063X 0000-0001-6691-9253 https://hdl.handle.net/10356/150186 10.1002/jbio.201900073 31100192 2-s2.0-85067082534 9 12 e201900073 en NMRC/CBRG/0036/2013 NRF-CRP13-2014-05 2018-T1-001-144 H17/01/a0/008 NAM/15005 Journal of Biophotonics This is the accepted version of the following article: Chen, S., Liu, X., Wang, N., Ding, Q., Wang, X., Ge, X., Bo, E., Yu, X., Yu, H., Xu, C. & Liu, L. (2019). Contrast of nuclei in stratified squamous epithelium in optical coherence tomography images at 800 nm. Journal of Biophotonics, 12(9), which has been published in final form at https://doi.org/10.1002/jbio.201900073. This article may be used for non-commercial purposes in accordance with the Wiley Self-Archiving Policy [https://authorservices.wiley.com/authorresources/Journal-Authors/licensing/self-archiving.html]. application/pdf |
| spellingShingle | Engineering::Bioengineering Epithelial Cancer Nucleus Chen, Si Liu, Xinyu Wang, Nanshuo Ding, Qianshan Wang, Xianghong Ge, Xin Bo, En Yu, Xiaojun Yu, Honggang Xu, Chenjie Liu, Linbo Contrast of nuclei in stratified squamous epithelium in optical coherence tomography images at 800 nm |
| title | Contrast of nuclei in stratified squamous epithelium in optical coherence tomography images at 800 nm |
| title_full | Contrast of nuclei in stratified squamous epithelium in optical coherence tomography images at 800 nm |
| title_fullStr | Contrast of nuclei in stratified squamous epithelium in optical coherence tomography images at 800 nm |
| title_full_unstemmed | Contrast of nuclei in stratified squamous epithelium in optical coherence tomography images at 800 nm |
| title_short | Contrast of nuclei in stratified squamous epithelium in optical coherence tomography images at 800 nm |
| title_sort | contrast of nuclei in stratified squamous epithelium in optical coherence tomography images at 800 nm |
| topic | Engineering::Bioengineering Epithelial Cancer Nucleus |
| url | https://hdl.handle.net/10356/150186 |
| work_keys_str_mv | AT chensi contrastofnucleiinstratifiedsquamousepitheliuminopticalcoherencetomographyimagesat800nm AT liuxinyu contrastofnucleiinstratifiedsquamousepitheliuminopticalcoherencetomographyimagesat800nm AT wangnanshuo contrastofnucleiinstratifiedsquamousepitheliuminopticalcoherencetomographyimagesat800nm AT dingqianshan contrastofnucleiinstratifiedsquamousepitheliuminopticalcoherencetomographyimagesat800nm AT wangxianghong contrastofnucleiinstratifiedsquamousepitheliuminopticalcoherencetomographyimagesat800nm AT gexin contrastofnucleiinstratifiedsquamousepitheliuminopticalcoherencetomographyimagesat800nm AT boen contrastofnucleiinstratifiedsquamousepitheliuminopticalcoherencetomographyimagesat800nm AT yuxiaojun contrastofnucleiinstratifiedsquamousepitheliuminopticalcoherencetomographyimagesat800nm AT yuhonggang contrastofnucleiinstratifiedsquamousepitheliuminopticalcoherencetomographyimagesat800nm AT xuchenjie contrastofnucleiinstratifiedsquamousepitheliuminopticalcoherencetomographyimagesat800nm AT liulinbo contrastofnucleiinstratifiedsquamousepitheliuminopticalcoherencetomographyimagesat800nm |