Oxidative stress response plays a role in antibiotic tolerance of Streptococcus mutans biofilms
Knowledge about biofilm-associated antibiotic tolerance mechanisms is warranted in order to develop effective treatments against biofilm infections. We performed a screen of a Streptococcus mutans transposon mutant library for mutants with reduced biofilm-associated antimicrobial tolerance, and foun...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2021
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/150228 |
| _version_ | 1811682744968675328 |
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| author | Nilsson, Martin Jakobsen, Tim Holm Givskov, Michael Twetman, Svante Tolker-Nielsen, Tim |
| author2 | Singapore Centre for Environmental Life Sciences and Engineering |
| author_facet | Singapore Centre for Environmental Life Sciences and Engineering Nilsson, Martin Jakobsen, Tim Holm Givskov, Michael Twetman, Svante Tolker-Nielsen, Tim |
| author_sort | Nilsson, Martin |
| collection | NTU |
| description | Knowledge about biofilm-associated antibiotic tolerance mechanisms is warranted in order to develop effective treatments against biofilm infections. We performed a screen of a Streptococcus mutans transposon mutant library for mutants with reduced biofilm-associated antimicrobial tolerance, and found that the spxA1 gene plays a role in tolerance towards gentamicin and other antibiotics such as vancomycin and linezolid. SpxA1 is a regulator of genes involved in the oxidative stress response in S. mutans. The oxidative stress response genes gor and ahpC were found to be up-regulated upon antibiotic treatment of S. mutans wild-type biofilms, but not spxA1 mutant biofilms. The gor gene product catalyses the formation of glutathione which functions as an important antioxidant during oxidative stress, and accordingly biofilm-associated antibiotic tolerance of the spxA1 mutant could be restored by exogenous addition of glutathione. Our results indicate that the oxidative stress response plays a role in biofilm-associated antibiotic tolerance of S. mutans, and add to the on-going debate on the role of reactive oxygen species in antibiotic mediated killing of bacteria. |
| first_indexed | 2024-10-01T04:01:43Z |
| format | Journal Article |
| id | ntu-10356/150228 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:01:43Z |
| publishDate | 2021 |
| record_format | dspace |
| spelling | ntu-10356/1502282021-06-12T20:11:18Z Oxidative stress response plays a role in antibiotic tolerance of Streptococcus mutans biofilms Nilsson, Martin Jakobsen, Tim Holm Givskov, Michael Twetman, Svante Tolker-Nielsen, Tim Singapore Centre for Environmental Life Sciences and Engineering Engineering::Bioengineering Streptococcus Mutans Biofilm Knowledge about biofilm-associated antibiotic tolerance mechanisms is warranted in order to develop effective treatments against biofilm infections. We performed a screen of a Streptococcus mutans transposon mutant library for mutants with reduced biofilm-associated antimicrobial tolerance, and found that the spxA1 gene plays a role in tolerance towards gentamicin and other antibiotics such as vancomycin and linezolid. SpxA1 is a regulator of genes involved in the oxidative stress response in S. mutans. The oxidative stress response genes gor and ahpC were found to be up-regulated upon antibiotic treatment of S. mutans wild-type biofilms, but not spxA1 mutant biofilms. The gor gene product catalyses the formation of glutathione which functions as an important antioxidant during oxidative stress, and accordingly biofilm-associated antibiotic tolerance of the spxA1 mutant could be restored by exogenous addition of glutathione. Our results indicate that the oxidative stress response plays a role in biofilm-associated antibiotic tolerance of S. mutans, and add to the on-going debate on the role of reactive oxygen species in antibiotic mediated killing of bacteria. Published version 2021-06-09T08:45:07Z 2021-06-09T08:45:07Z 2019 Journal Article Nilsson, M., Jakobsen, T. H., Givskov, M., Twetman, S. & Tolker-Nielsen, T. (2019). Oxidative stress response plays a role in antibiotic tolerance of Streptococcus mutans biofilms. Microbiology, 165(3), 334-342. https://dx.doi.org/10.1099/mic.0.000773 1350-0872 https://hdl.handle.net/10356/150228 10.1099/mic.0.000773 30663959 2-s2.0-85063236832 3 165 334 342 en Microbiology © 2019 The Authors (published by Microbiology Society). This is an open-access article distributed under the terms of the Creative Commons Attribution License. application/pdf |
| spellingShingle | Engineering::Bioengineering Streptococcus Mutans Biofilm Nilsson, Martin Jakobsen, Tim Holm Givskov, Michael Twetman, Svante Tolker-Nielsen, Tim Oxidative stress response plays a role in antibiotic tolerance of Streptococcus mutans biofilms |
| title | Oxidative stress response plays a role in antibiotic tolerance of Streptococcus mutans biofilms |
| title_full | Oxidative stress response plays a role in antibiotic tolerance of Streptococcus mutans biofilms |
| title_fullStr | Oxidative stress response plays a role in antibiotic tolerance of Streptococcus mutans biofilms |
| title_full_unstemmed | Oxidative stress response plays a role in antibiotic tolerance of Streptococcus mutans biofilms |
| title_short | Oxidative stress response plays a role in antibiotic tolerance of Streptococcus mutans biofilms |
| title_sort | oxidative stress response plays a role in antibiotic tolerance of streptococcus mutans biofilms |
| topic | Engineering::Bioengineering Streptococcus Mutans Biofilm |
| url | https://hdl.handle.net/10356/150228 |
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