Liquid-crystalline hydroxyapatite/polymer nanorod hybrids : potential bioplatform for photodynamic therapy and cellular scaffolds
Recently, we found that self-organization of hydroxyapatite (HAp) with poly(acrylic acid) (PAA) leads to the formation of liquid-crystalline (LC) nanorod hybrids that form aligned films and show stimuli-responsive properties. Here, we demonstrate that these biocompatible HAp/PAA hybrid nanorods repr...
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| Format: | Journal Article |
| Language: | English |
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2021
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| Online Access: | https://hdl.handle.net/10356/151376 |
| _version_ | 1811692228339302400 |
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| author | Nakayama, Masanari Lim, Wei Qi Kajiyama, Satoshi Kumamoto, Akihito Ikuhara, Yuichi Kato, Takashi Zhao, Yanli |
| author2 | School of Physical and Mathematical Sciences |
| author_facet | School of Physical and Mathematical Sciences Nakayama, Masanari Lim, Wei Qi Kajiyama, Satoshi Kumamoto, Akihito Ikuhara, Yuichi Kato, Takashi Zhao, Yanli |
| author_sort | Nakayama, Masanari |
| collection | NTU |
| description | Recently, we found that self-organization of hydroxyapatite (HAp) with poly(acrylic acid) (PAA) leads to the formation of liquid-crystalline (LC) nanorod hybrids that form aligned films and show stimuli-responsive properties. Here, we demonstrate that these biocompatible HAp/PAA hybrid nanorods represent a platform technology as drug nanocarriers for photodynamic cancer therapy and as bioscaffolds for the control of cellular alignment and growth. To use hybrid nanorods as a drug nanocarrier, we introduced methylene blue (MB), a typical photosensitizer for photodynamic therapy, into the PAA nanolayer covering the surface of the HAp nanocrystals through electrostatic interactions. The stable MB-loaded HAp/PAA hybrid nanorods efficiently produced singlet oxygen from MB upon light irradiation and showed remarkable photodynamic therapeutic effects in cancer cells. Moreover, taking advantage of the mechanically responsive LC alignment properties of the HAp/PAA hybrid nanorods, macroscopically oriented bioscaffolds were prepared through a spin-coating process. The cells cultured on the oriented scaffolds showed cellular alignment and elongation along the oriented direction of the hybrid nanorods. The HAp/PAA hybrid nanorods demonstrate potential in drug delivery and tissue engineering. These unique LC HAp/PAA hybrid nanorods have significant potential as a platform for the development of various types of biomaterial. |
| first_indexed | 2024-10-01T06:32:27Z |
| format | Journal Article |
| id | ntu-10356/151376 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:32:27Z |
| publishDate | 2021 |
| record_format | dspace |
| spelling | ntu-10356/1513762021-07-23T06:03:18Z Liquid-crystalline hydroxyapatite/polymer nanorod hybrids : potential bioplatform for photodynamic therapy and cellular scaffolds Nakayama, Masanari Lim, Wei Qi Kajiyama, Satoshi Kumamoto, Akihito Ikuhara, Yuichi Kato, Takashi Zhao, Yanli School of Physical and Mathematical Sciences Science::Chemistry Photodynamic Therapy Drug Nanocarrier Drug Nanocarrier Recently, we found that self-organization of hydroxyapatite (HAp) with poly(acrylic acid) (PAA) leads to the formation of liquid-crystalline (LC) nanorod hybrids that form aligned films and show stimuli-responsive properties. Here, we demonstrate that these biocompatible HAp/PAA hybrid nanorods represent a platform technology as drug nanocarriers for photodynamic cancer therapy and as bioscaffolds for the control of cellular alignment and growth. To use hybrid nanorods as a drug nanocarrier, we introduced methylene blue (MB), a typical photosensitizer for photodynamic therapy, into the PAA nanolayer covering the surface of the HAp nanocrystals through electrostatic interactions. The stable MB-loaded HAp/PAA hybrid nanorods efficiently produced singlet oxygen from MB upon light irradiation and showed remarkable photodynamic therapeutic effects in cancer cells. Moreover, taking advantage of the mechanically responsive LC alignment properties of the HAp/PAA hybrid nanorods, macroscopically oriented bioscaffolds were prepared through a spin-coating process. The cells cultured on the oriented scaffolds showed cellular alignment and elongation along the oriented direction of the hybrid nanorods. The HAp/PAA hybrid nanorods demonstrate potential in drug delivery and tissue engineering. These unique LC HAp/PAA hybrid nanorods have significant potential as a platform for the development of various types of biomaterial. National Research Foundation (NRF) This study was partly supported by JSPS KAKENHI Grant Numbers JP15H02179 and JP17J09259. M.N. is grateful for financial support from a Japan Society for the Promotion of Science (JSPS) Research Fellowship for Young Scientists and the JSPS Program for Leading Graduate Schools (MERIT). The authors are grateful to Dr. S.Y. for performing the ζ-potential measurements. TEM observations were conducted at the Advanced Characterization Nanotechnology Platform at the University of Tokyo, which is supported by the “Nanotechnology Platform” of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. This work was also partially supported by the Singapore National Research Foundation Investigatorship (No. NRF-NRFI2018-03). 2021-07-23T06:03:17Z 2021-07-23T06:03:17Z 2019 Journal Article Nakayama, M., Lim, W. Q., Kajiyama, S., Kumamoto, A., Ikuhara, Y., Kato, T. & Zhao, Y. (2019). Liquid-crystalline hydroxyapatite/polymer nanorod hybrids : potential bioplatform for photodynamic therapy and cellular scaffolds. ACS Applied Materials and Interfaces, 11(19), 17759-17765. https://dx.doi.org/10.1021/acsami.9b02485 1944-8244 0000-0001-7023-3801 0000-0002-2200-7524 0000-0002-9730-3045 0000-0003-3886-005X 0000-0002-0571-0883 0000-0002-9231-8360 https://hdl.handle.net/10356/151376 10.1021/acsami.9b02485 31010284 2-s2.0-85065773146 19 11 17759 17765 en NRF-NRFI2018-03 ACS Applied Materials and Interfaces © 2019 American Chemical Society. All rights reserved. |
| spellingShingle | Science::Chemistry Photodynamic Therapy Drug Nanocarrier Drug Nanocarrier Nakayama, Masanari Lim, Wei Qi Kajiyama, Satoshi Kumamoto, Akihito Ikuhara, Yuichi Kato, Takashi Zhao, Yanli Liquid-crystalline hydroxyapatite/polymer nanorod hybrids : potential bioplatform for photodynamic therapy and cellular scaffolds |
| title | Liquid-crystalline hydroxyapatite/polymer nanorod hybrids : potential bioplatform for photodynamic therapy and cellular scaffolds |
| title_full | Liquid-crystalline hydroxyapatite/polymer nanorod hybrids : potential bioplatform for photodynamic therapy and cellular scaffolds |
| title_fullStr | Liquid-crystalline hydroxyapatite/polymer nanorod hybrids : potential bioplatform for photodynamic therapy and cellular scaffolds |
| title_full_unstemmed | Liquid-crystalline hydroxyapatite/polymer nanorod hybrids : potential bioplatform for photodynamic therapy and cellular scaffolds |
| title_short | Liquid-crystalline hydroxyapatite/polymer nanorod hybrids : potential bioplatform for photodynamic therapy and cellular scaffolds |
| title_sort | liquid crystalline hydroxyapatite polymer nanorod hybrids potential bioplatform for photodynamic therapy and cellular scaffolds |
| topic | Science::Chemistry Photodynamic Therapy Drug Nanocarrier Drug Nanocarrier |
| url | https://hdl.handle.net/10356/151376 |
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