The role of Angiopoietin-like protein 4 in macrophage activation
Angiopoietin-like protein 4 (Angptl4) is a matricellular protein that associates with extracellular matrix proteins, mediating complex cell-cell, and cell-matrix interactions. It has been implicated in various inflammation-associated diseases, including atherosclerosis, pulmonary damage, and wound h...
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| Format: | Thesis-Doctor of Philosophy |
| Language: | English |
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Nanyang Technological University
2021
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| Online Access: | https://hdl.handle.net/10356/151419 |
| _version_ | 1826123037645209600 |
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| author | Wee, Jonathan Wei Kiat |
| author2 | Tan Nguan Soon |
| author_facet | Tan Nguan Soon Wee, Jonathan Wei Kiat |
| author_sort | Wee, Jonathan Wei Kiat |
| collection | NTU |
| description | Angiopoietin-like protein 4 (Angptl4) is a matricellular protein that associates with extracellular matrix proteins, mediating complex cell-cell, and cell-matrix interactions. It has been implicated in various inflammation-associated diseases, including atherosclerosis, pulmonary damage, and wound healing. Studies have shown Angptl4 to be a key regulator of wound healing, affecting different processes like cell adhesion, migration, and angiogenesis. However, very few reports describe a direct role for Angptl4 in immune cell development and response. Using single-cell RNA sequencing to examine the temporal changes in the immune cell landscape of excisional wounds from wild type and Angplt4-knockout (Angplt4-/-) mice revealed that Angptl4-/- wounds had a stalled inflammatory phase. We identified interferon activated protein 202B (ifi202b) to be consistently upregulated in Angptl4-/- macrophages. We confirmed that ifb202b significantly impacted multiple gene networks involved in the cell fate of monocytes and the functions of monocyte-derived macrophages. Taken altogether, we show the importance of Angptl4 in macrophage function during wound healing and its parallels to diabetic wounds during deficiency. |
| first_indexed | 2024-10-01T05:58:04Z |
| format | Thesis-Doctor of Philosophy |
| id | ntu-10356/151419 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:58:04Z |
| publishDate | 2021 |
| publisher | Nanyang Technological University |
| record_format | dspace |
| spelling | ntu-10356/1514192023-02-28T18:42:20Z The role of Angiopoietin-like protein 4 in macrophage activation Wee, Jonathan Wei Kiat Tan Nguan Soon School of Biological Sciences NSTan@ntu.edu.sg Science::Biological sciences Angiopoietin-like protein 4 (Angptl4) is a matricellular protein that associates with extracellular matrix proteins, mediating complex cell-cell, and cell-matrix interactions. It has been implicated in various inflammation-associated diseases, including atherosclerosis, pulmonary damage, and wound healing. Studies have shown Angptl4 to be a key regulator of wound healing, affecting different processes like cell adhesion, migration, and angiogenesis. However, very few reports describe a direct role for Angptl4 in immune cell development and response. Using single-cell RNA sequencing to examine the temporal changes in the immune cell landscape of excisional wounds from wild type and Angplt4-knockout (Angplt4-/-) mice revealed that Angptl4-/- wounds had a stalled inflammatory phase. We identified interferon activated protein 202B (ifi202b) to be consistently upregulated in Angptl4-/- macrophages. We confirmed that ifb202b significantly impacted multiple gene networks involved in the cell fate of monocytes and the functions of monocyte-derived macrophages. Taken altogether, we show the importance of Angptl4 in macrophage function during wound healing and its parallels to diabetic wounds during deficiency. Doctor of Philosophy 2021-06-24T12:20:46Z 2021-06-24T12:20:46Z 2020 Thesis-Doctor of Philosophy Wee, J. W. K. (2020). The role of Angiopoietin-like protein 4 in macrophage activation. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/151419 https://hdl.handle.net/10356/151419 10.32657/10356/151419 en This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0). application/pdf Nanyang Technological University |
| spellingShingle | Science::Biological sciences Wee, Jonathan Wei Kiat The role of Angiopoietin-like protein 4 in macrophage activation |
| title | The role of Angiopoietin-like protein 4 in macrophage activation |
| title_full | The role of Angiopoietin-like protein 4 in macrophage activation |
| title_fullStr | The role of Angiopoietin-like protein 4 in macrophage activation |
| title_full_unstemmed | The role of Angiopoietin-like protein 4 in macrophage activation |
| title_short | The role of Angiopoietin-like protein 4 in macrophage activation |
| title_sort | role of angiopoietin like protein 4 in macrophage activation |
| topic | Science::Biological sciences |
| url | https://hdl.handle.net/10356/151419 |
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