Unravelling the aptamer-analyte interaction dynamics through fluorescence quenching in graphene quantum dots (GQDS) based homogeneous assays
Graphene quantum dots (GQDs) are used here as a biosensing platform for the recognition of the major food contaminant ochratoxin A (OTA), with a fluorescently labelled DNA aptamer (FAM OTA aptamer) functioning as the biorecognition element. The detection principle lies in the formation of noncovalen...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2021
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| Online Access: | https://hdl.handle.net/10356/151663 |
| _version_ | 1826114744551997440 |
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| author | Ang, Wei Li Bonanni, Alessandra |
| author2 | School of Physical and Mathematical Sciences |
| author_facet | School of Physical and Mathematical Sciences Ang, Wei Li Bonanni, Alessandra |
| author_sort | Ang, Wei Li |
| collection | NTU |
| description | Graphene quantum dots (GQDs) are used here as a biosensing platform for the recognition of the major food contaminant ochratoxin A (OTA), with a fluorescently labelled DNA aptamer (FAM OTA aptamer) functioning as the biorecognition element. The detection principle lies in the formation of noncovalent interactions between the FAM OTA aptamer and the GQD surface, and the consequent fluorescence quenching. The further change in the fluorescence signal, induced by the formation of the FAM OTA Aptamer/OTA conjugate during the detection step, could then be correlated to the presence and concentration of the target analyte. Upon tuning the concentration of GQDs, a switch in the biorecognition mechanism occurred. Specifically, while a lower GQD concentration (0.060 mg/mL) resulted in a restoration of the fluorescence intensity upon incubation with OTA, a higher GQD concentration (0.150 mg/mL) provided a further quenching of the final fluorescence intensity. Upon further calibration study, it was discovered that the latter mechanism provided a better option in terms of linearity of response, detection limit and selectivity. |
| first_indexed | 2024-10-01T03:44:09Z |
| format | Journal Article |
| id | ntu-10356/151663 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:44:09Z |
| publishDate | 2021 |
| record_format | dspace |
| spelling | ntu-10356/1516632021-07-09T07:45:21Z Unravelling the aptamer-analyte interaction dynamics through fluorescence quenching in graphene quantum dots (GQDS) based homogeneous assays Ang, Wei Li Bonanni, Alessandra School of Physical and Mathematical Sciences Science::Chemistry Aptamers Biosensors Graphene quantum dots (GQDs) are used here as a biosensing platform for the recognition of the major food contaminant ochratoxin A (OTA), with a fluorescently labelled DNA aptamer (FAM OTA aptamer) functioning as the biorecognition element. The detection principle lies in the formation of noncovalent interactions between the FAM OTA aptamer and the GQD surface, and the consequent fluorescence quenching. The further change in the fluorescence signal, induced by the formation of the FAM OTA Aptamer/OTA conjugate during the detection step, could then be correlated to the presence and concentration of the target analyte. Upon tuning the concentration of GQDs, a switch in the biorecognition mechanism occurred. Specifically, while a lower GQD concentration (0.060 mg/mL) resulted in a restoration of the fluorescence intensity upon incubation with OTA, a higher GQD concentration (0.150 mg/mL) provided a further quenching of the final fluorescence intensity. Upon further calibration study, it was discovered that the latter mechanism provided a better option in terms of linearity of response, detection limit and selectivity. Nanyang Technological University A. B. acknowledges Nanyang Technological University for the financial support. 2021-07-09T07:45:20Z 2021-07-09T07:45:20Z 2019 Journal Article Ang, W. L. & Bonanni, A. (2019). Unravelling the aptamer-analyte interaction dynamics through fluorescence quenching in graphene quantum dots (GQDS) based homogeneous assays. ChemPlusChem, 84(4), 420-426. https://dx.doi.org/10.1002/cplu.201900146 2192-6506 0000-0001-8214-6748 https://hdl.handle.net/10356/151663 10.1002/cplu.201900146 31939208 2-s2.0-85064897249 4 84 420 426 en ChemPlusChem © 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. All rights reserved. |
| spellingShingle | Science::Chemistry Aptamers Biosensors Ang, Wei Li Bonanni, Alessandra Unravelling the aptamer-analyte interaction dynamics through fluorescence quenching in graphene quantum dots (GQDS) based homogeneous assays |
| title | Unravelling the aptamer-analyte interaction dynamics through fluorescence quenching in graphene quantum dots (GQDS) based homogeneous assays |
| title_full | Unravelling the aptamer-analyte interaction dynamics through fluorescence quenching in graphene quantum dots (GQDS) based homogeneous assays |
| title_fullStr | Unravelling the aptamer-analyte interaction dynamics through fluorescence quenching in graphene quantum dots (GQDS) based homogeneous assays |
| title_full_unstemmed | Unravelling the aptamer-analyte interaction dynamics through fluorescence quenching in graphene quantum dots (GQDS) based homogeneous assays |
| title_short | Unravelling the aptamer-analyte interaction dynamics through fluorescence quenching in graphene quantum dots (GQDS) based homogeneous assays |
| title_sort | unravelling the aptamer analyte interaction dynamics through fluorescence quenching in graphene quantum dots gqds based homogeneous assays |
| topic | Science::Chemistry Aptamers Biosensors |
| url | https://hdl.handle.net/10356/151663 |
| work_keys_str_mv | AT angweili unravellingtheaptameranalyteinteractiondynamicsthroughfluorescencequenchingingraphenequantumdotsgqdsbasedhomogeneousassays AT bonannialessandra unravellingtheaptameranalyteinteractiondynamicsthroughfluorescencequenchingingraphenequantumdotsgqdsbasedhomogeneousassays |