Multiplex optical urinalysis for early detection of drug-induced kidney injury
Drug-induced kidney injury (DIKI) is a significant contributor of both acute and chronic kidney injury and remains a major concern in drug development and clinical care. However, current clinical diagnostic methods often fail to accurately and timely detect nephrotoxicity. This study reports the dev...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | English |
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2021
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| Online Access: | https://hdl.handle.net/10356/152066 |
| _version_ | 1811690955762302976 |
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| author | Cheng, Penghui Miao, Qingqing Huang, Jiaguo Li, Jingchao Pu, Kanyi |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Cheng, Penghui Miao, Qingqing Huang, Jiaguo Li, Jingchao Pu, Kanyi |
| author_sort | Cheng, Penghui |
| collection | NTU |
| description | Drug-induced kidney injury (DIKI) is a significant contributor of both acute and chronic kidney injury and remains a major concern in drug development and clinical care. However, current clinical diagnostic methods often fail to accurately and timely detect nephrotoxicity. This study reports the development of activatable molecular urinary reporters (MURs) that are able to specifically detect urinary biomarkers including γ-glutamyl transferase (GGT), alanine aminopeptidase (AAP), and N-acetyl-β-d-glucosaminidase (NAG). By virtue of their discrete absorption and emission properties, the mixture of MURs can serve as a cocktail sensor for multiplex optical urinalysis in the mouse models of drug-induced acute kidney injury (AKI) and chronic kidney disease (CKD). The MURs cocktail not only detects nephrotoxicity earlier than the tested clinical diagnostic methods in drug-induced AKI and CKD mice models, but also possesses a higher diagnostic accuracy. Therefore, MURs hold great promise for detection of kidney function in both preclinical drug screening and clinical settings. |
| first_indexed | 2024-10-01T06:12:13Z |
| format | Journal Article |
| id | ntu-10356/152066 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:12:13Z |
| publishDate | 2021 |
| record_format | dspace |
| spelling | ntu-10356/1520662021-09-01T06:36:59Z Multiplex optical urinalysis for early detection of drug-induced kidney injury Cheng, Penghui Miao, Qingqing Huang, Jiaguo Li, Jingchao Pu, Kanyi School of Chemical and Biomedical Engineering Engineering::Chemical engineering Anatomy Fluorescence Drug-induced kidney injury (DIKI) is a significant contributor of both acute and chronic kidney injury and remains a major concern in drug development and clinical care. However, current clinical diagnostic methods often fail to accurately and timely detect nephrotoxicity. This study reports the development of activatable molecular urinary reporters (MURs) that are able to specifically detect urinary biomarkers including γ-glutamyl transferase (GGT), alanine aminopeptidase (AAP), and N-acetyl-β-d-glucosaminidase (NAG). By virtue of their discrete absorption and emission properties, the mixture of MURs can serve as a cocktail sensor for multiplex optical urinalysis in the mouse models of drug-induced acute kidney injury (AKI) and chronic kidney disease (CKD). The MURs cocktail not only detects nephrotoxicity earlier than the tested clinical diagnostic methods in drug-induced AKI and CKD mice models, but also possesses a higher diagnostic accuracy. Therefore, MURs hold great promise for detection of kidney function in both preclinical drug screening and clinical settings. Ministry of Education (MOE) Nanyang Technological University K.P. thanks the Nanyang Technological University (Start-Up grant: M4081627), Singapore Ministry of Education Academic Research Fund Tier 1 (2017-T1-002-134, RG147/17; 2019-T1-002-045, and RG125/19), and Academic Research Fund Tier 2 (MOE2018-T2-2-042) for financial support. 2021-09-01T06:36:59Z 2021-09-01T06:36:59Z 2020 Journal Article Cheng, P., Miao, Q., Huang, J., Li, J. & Pu, K. (2020). Multiplex optical urinalysis for early detection of drug-induced kidney injury. Analytical Chemistry, 92(8), 6166-6172. https://dx.doi.org/10.1021/acs.analchem.0c00989 0003-2700 https://hdl.handle.net/10356/152066 10.1021/acs.analchem.0c00989 32241110 2-s2.0-85084658361 8 92 6166 6172 en M4081627 017-T1-002-134 RG147/17 2019-T1-002-045 RG125/19 MOE2018-T2-2-042 Analytical Chemistry © 2020 American Chemical Society. All rights reserved. |
| spellingShingle | Engineering::Chemical engineering Anatomy Fluorescence Cheng, Penghui Miao, Qingqing Huang, Jiaguo Li, Jingchao Pu, Kanyi Multiplex optical urinalysis for early detection of drug-induced kidney injury |
| title | Multiplex optical urinalysis for early detection of drug-induced kidney injury |
| title_full | Multiplex optical urinalysis for early detection of drug-induced kidney injury |
| title_fullStr | Multiplex optical urinalysis for early detection of drug-induced kidney injury |
| title_full_unstemmed | Multiplex optical urinalysis for early detection of drug-induced kidney injury |
| title_short | Multiplex optical urinalysis for early detection of drug-induced kidney injury |
| title_sort | multiplex optical urinalysis for early detection of drug induced kidney injury |
| topic | Engineering::Chemical engineering Anatomy Fluorescence |
| url | https://hdl.handle.net/10356/152066 |
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