Comparison of retinal vessel diameter measurements from swept-source OCT angiography and adaptive optics ophthalmoscope
Background/ims: To compare the retinal vessel diameter measurements obtained from the swept-source optical coherence tomography angiography (OCTA; Plex Elite 9000, Carl Zeiss Meditec, USA) and adaptive optics ophthalmoscope (AOO; RTX1, Imagine Eyes, France). Methods: Fifteen healthy subjects, 67% wo...
| Main Authors: | , , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2021
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| Online Access: | https://hdl.handle.net/10356/152181 |
| _version_ | 1826129284346937344 |
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| author | Yao, Xinwen Ke, Mengyuan Ho, Yijie Lin, Emily Wong, Damon Wing Kee Tan, Bingyao Schmetterer, Leopold Chua, Jacqueline |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Yao, Xinwen Ke, Mengyuan Ho, Yijie Lin, Emily Wong, Damon Wing Kee Tan, Bingyao Schmetterer, Leopold Chua, Jacqueline |
| author_sort | Yao, Xinwen |
| collection | NTU |
| description | Background/ims: To compare the retinal vessel diameter measurements obtained from the swept-source optical coherence tomography angiography (OCTA; Plex Elite 9000, Carl Zeiss Meditec, USA) and adaptive optics ophthalmoscope (AOO; RTX1, Imagine Eyes, France). Methods: Fifteen healthy subjects, 67% women, mean age (SD) 30.87 (6.19) years, were imaged using OCTA and AOO by a single experienced operator on the same day. Each eye was scanned using two OCTA protocols (3×3 mm² and 9×9 mm²) and two to five AOO scans (1.2×1.2 mm²). The OCTA and AOO scans were scaled to the same pixel resolution. Two independent graders measured the vessel diameter at the same location on the region-of-interest in the three coregistered scans. Differences in vessel diameter measurements between the scans were assessed. Results: The inter-rater agreement was excellent for vessel diameter measurement in both OCTA protocols (ICC=0.92) and AOO (ICC=0.98). The measured vessel diameter was widest from the OCTA 3×3 mm² (55.2±16.3 µm), followed by OCTA 9×9 mm² (54.7±14.3 µm) and narrowest by the AOO (50.5±15.6 µm; p<0.001). Measurements obtained from both OCTA protocols were significantly wider than the AOO scan (OCTA 3×3 mm²: mean difference Δ=4.7 µm, p<0.001; OCTA 9×9 mm²: Δ=4.2 µm, p<0.001). For vessels >45 µm, it appeared to be larger in OCTA 3×3 mm² scan than the 9×9 mm² scan (Δ=1.9 µm; p=0.005), while vessels <45 µm appeared smaller in OCTA 3×3 mm² scan (Δ=−1.3 µm; p=0.009) Conclusions: The diameter of retinal vessels measured from OCTA scans were generally wider than that obtained from AOO scans. Different OCTA scan protocols may affect the vessel diameter measurements. This needs to be considered when OCTA measures such as vessel density are calculated. |
| first_indexed | 2024-10-01T07:38:07Z |
| format | Journal Article |
| id | ntu-10356/152181 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:38:07Z |
| publishDate | 2021 |
| record_format | dspace |
| spelling | ntu-10356/1521812023-12-29T06:52:33Z Comparison of retinal vessel diameter measurements from swept-source OCT angiography and adaptive optics ophthalmoscope Yao, Xinwen Ke, Mengyuan Ho, Yijie Lin, Emily Wong, Damon Wing Kee Tan, Bingyao Schmetterer, Leopold Chua, Jacqueline School of Chemical and Biomedical Engineering Singapore Eye Research Institute, Singapore National Eye Center Duke-NUS Medical School NTU Institute for Health Technologies SERI-NTU Advanced Ocular Engineering (STANCE) Engineering::Chemical engineering Imaging Retina Background/ims: To compare the retinal vessel diameter measurements obtained from the swept-source optical coherence tomography angiography (OCTA; Plex Elite 9000, Carl Zeiss Meditec, USA) and adaptive optics ophthalmoscope (AOO; RTX1, Imagine Eyes, France). Methods: Fifteen healthy subjects, 67% women, mean age (SD) 30.87 (6.19) years, were imaged using OCTA and AOO by a single experienced operator on the same day. Each eye was scanned using two OCTA protocols (3×3 mm² and 9×9 mm²) and two to five AOO scans (1.2×1.2 mm²). The OCTA and AOO scans were scaled to the same pixel resolution. Two independent graders measured the vessel diameter at the same location on the region-of-interest in the three coregistered scans. Differences in vessel diameter measurements between the scans were assessed. Results: The inter-rater agreement was excellent for vessel diameter measurement in both OCTA protocols (ICC=0.92) and AOO (ICC=0.98). The measured vessel diameter was widest from the OCTA 3×3 mm² (55.2±16.3 µm), followed by OCTA 9×9 mm² (54.7±14.3 µm) and narrowest by the AOO (50.5±15.6 µm; p<0.001). Measurements obtained from both OCTA protocols were significantly wider than the AOO scan (OCTA 3×3 mm²: mean difference Δ=4.7 µm, p<0.001; OCTA 9×9 mm²: Δ=4.2 µm, p<0.001). For vessels >45 µm, it appeared to be larger in OCTA 3×3 mm² scan than the 9×9 mm² scan (Δ=1.9 µm; p=0.005), while vessels <45 µm appeared smaller in OCTA 3×3 mm² scan (Δ=−1.3 µm; p=0.009) Conclusions: The diameter of retinal vessels measured from OCTA scans were generally wider than that obtained from AOO scans. Different OCTA scan protocols may affect the vessel diameter measurements. This needs to be considered when OCTA measures such as vessel density are calculated. National Medical Research Council (NMRC) Published version The study is funded by National Medical Research Council (grants CG/C010A/2017, OFLCG/004C/2018 and TA/MOH-000249-00/2018) and the Duke-NUS Medical School (Duke-NUS-KP(Coll)/2018/0009A), Singapore. 2021-08-04T02:42:11Z 2021-08-04T02:42:11Z 2021 Journal Article Yao, X., Ke, M., Ho, Y., Lin, E., Wong, D. W. K., Tan, B., Schmetterer, L. & Chua, J. (2021). Comparison of retinal vessel diameter measurements from swept-source OCT angiography and adaptive optics ophthalmoscope. British Journal of Ophthalmology, 105(3), 426-431. https://dx.doi.org/10.1136/bjophthalmol-2020-316111 0007-1161 https://hdl.handle.net/10356/152181 10.1136/bjophthalmol-2020-316111 32461263 2-s2.0-85085765783 3 105 426 431 en CG/C010A/2017 OFLCG/004C/2018 TA/MOH-000249-00/2018 Duke-NUS-KP(Coll)/2018/0009A British Journal of Ophthalmology © Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ. This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/. application/pdf |
| spellingShingle | Engineering::Chemical engineering Imaging Retina Yao, Xinwen Ke, Mengyuan Ho, Yijie Lin, Emily Wong, Damon Wing Kee Tan, Bingyao Schmetterer, Leopold Chua, Jacqueline Comparison of retinal vessel diameter measurements from swept-source OCT angiography and adaptive optics ophthalmoscope |
| title | Comparison of retinal vessel diameter measurements from swept-source OCT angiography and adaptive optics ophthalmoscope |
| title_full | Comparison of retinal vessel diameter measurements from swept-source OCT angiography and adaptive optics ophthalmoscope |
| title_fullStr | Comparison of retinal vessel diameter measurements from swept-source OCT angiography and adaptive optics ophthalmoscope |
| title_full_unstemmed | Comparison of retinal vessel diameter measurements from swept-source OCT angiography and adaptive optics ophthalmoscope |
| title_short | Comparison of retinal vessel diameter measurements from swept-source OCT angiography and adaptive optics ophthalmoscope |
| title_sort | comparison of retinal vessel diameter measurements from swept source oct angiography and adaptive optics ophthalmoscope |
| topic | Engineering::Chemical engineering Imaging Retina |
| url | https://hdl.handle.net/10356/152181 |
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