Functional redundancy and plasticity in staphylococcus aureus vitamin B1 metabolism ensure stable colonisation in the environment and in the host
In this study, we explored fastidious metabolic pathways of Staphylococcus aureus and showed that the organism is auxotrophic for thiamine. Thiamine plays a key role in carbohydrate metabolism and is essential for ATP synthesis in both prokaryotes and eukaryotes. We demonstrated that thiamine depriv...
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| Format: | Thesis-Doctor of Philosophy |
| Language: | English |
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Nanyang Technological University
2021
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| Online Access: | https://hdl.handle.net/10356/152806 |
| _version_ | 1826122940235644928 |
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| author | Yeo, Benny Ken Yee |
| author2 | Kevin Pethe |
| author_facet | Kevin Pethe Yeo, Benny Ken Yee |
| author_sort | Yeo, Benny Ken Yee |
| collection | NTU |
| description | In this study, we explored fastidious metabolic pathways of Staphylococcus aureus and showed that the organism is auxotrophic for thiamine. Thiamine plays a key role in carbohydrate metabolism and is essential for ATP synthesis in both prokaryotes and eukaryotes. We demonstrated that thiamine deprivation is lethal in S. aureus and explored the essential pathway involved in vitamin biosynthesis and transport. Moreover, we have shown that thiVWX is a thiamine, TMP, and TPP transporter instead of a previously hypothesized HMP (thiamine precursor) transporter. Lastly, we also showed how the S. aureus thiamine pathway redundancy is necessary for S. aureus survival in different environmental niches. With multi-drug resistant (MDR) pathogen becoming a global issue and the lack of new drug discovery, our results open up new revenue for developing drugs against the MDR pathogen. |
| first_indexed | 2024-10-01T05:56:29Z |
| format | Thesis-Doctor of Philosophy |
| id | ntu-10356/152806 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:56:29Z |
| publishDate | 2021 |
| publisher | Nanyang Technological University |
| record_format | dspace |
| spelling | ntu-10356/1528062023-03-05T17:09:34Z Functional redundancy and plasticity in staphylococcus aureus vitamin B1 metabolism ensure stable colonisation in the environment and in the host Yeo, Benny Ken Yee Kevin Pethe Lee Kong Chian School of Medicine (LKCMedicine) kevin.pethe@ntu.edu.sg Science::Medicine In this study, we explored fastidious metabolic pathways of Staphylococcus aureus and showed that the organism is auxotrophic for thiamine. Thiamine plays a key role in carbohydrate metabolism and is essential for ATP synthesis in both prokaryotes and eukaryotes. We demonstrated that thiamine deprivation is lethal in S. aureus and explored the essential pathway involved in vitamin biosynthesis and transport. Moreover, we have shown that thiVWX is a thiamine, TMP, and TPP transporter instead of a previously hypothesized HMP (thiamine precursor) transporter. Lastly, we also showed how the S. aureus thiamine pathway redundancy is necessary for S. aureus survival in different environmental niches. With multi-drug resistant (MDR) pathogen becoming a global issue and the lack of new drug discovery, our results open up new revenue for developing drugs against the MDR pathogen. Doctor of Philosophy 2021-10-04T00:57:04Z 2021-10-04T00:57:04Z 2020 Thesis-Doctor of Philosophy Yeo, B. K. Y. (2020). Functional redundancy and plasticity in staphylococcus aureus vitamin B1 metabolism ensure stable colonisation in the environment and in the host. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/152806 https://hdl.handle.net/10356/152806 10.32657/10356/152806 en This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0). application/pdf Nanyang Technological University |
| spellingShingle | Science::Medicine Yeo, Benny Ken Yee Functional redundancy and plasticity in staphylococcus aureus vitamin B1 metabolism ensure stable colonisation in the environment and in the host |
| title | Functional redundancy and plasticity in staphylococcus aureus vitamin B1 metabolism ensure stable colonisation in the environment and in the host |
| title_full | Functional redundancy and plasticity in staphylococcus aureus vitamin B1 metabolism ensure stable colonisation in the environment and in the host |
| title_fullStr | Functional redundancy and plasticity in staphylococcus aureus vitamin B1 metabolism ensure stable colonisation in the environment and in the host |
| title_full_unstemmed | Functional redundancy and plasticity in staphylococcus aureus vitamin B1 metabolism ensure stable colonisation in the environment and in the host |
| title_short | Functional redundancy and plasticity in staphylococcus aureus vitamin B1 metabolism ensure stable colonisation in the environment and in the host |
| title_sort | functional redundancy and plasticity in staphylococcus aureus vitamin b1 metabolism ensure stable colonisation in the environment and in the host |
| topic | Science::Medicine |
| url | https://hdl.handle.net/10356/152806 |
| work_keys_str_mv | AT yeobennykenyee functionalredundancyandplasticityinstaphylococcusaureusvitaminb1metabolismensurestablecolonisationintheenvironmentandinthehost |