Integrating signaling pathways to pattern cells of the fin fold
During zebrafish median fin fold (MFF) development, mesoderm-derived mesenchymal fibroblasts migrate outwards into the fin fold, towards the Apical Ectodermal Ridge (AER). The Platelet Derived Growth Factor (PDGF) and Bone Morphogenetic Protein (BMP) are small diffusible ligands expressed in the AER...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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Nanyang Technological University
2021
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| Online Access: | https://hdl.handle.net/10356/153141 |
| _version_ | 1826122471301971968 |
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| author | Ho, Charmaine Min |
| author2 | Tom James Carney |
| author_facet | Tom James Carney Ho, Charmaine Min |
| author_sort | Ho, Charmaine Min |
| collection | NTU |
| description | During zebrafish median fin fold (MFF) development, mesoderm-derived mesenchymal fibroblasts migrate outwards into the fin fold, towards the Apical Ectodermal Ridge (AER). The Platelet Derived Growth Factor (PDGF) and Bone Morphogenetic Protein (BMP) are small diffusible ligands expressed in the AER, with their corresponding receptors expressed in the fin mesenchyme, and are suspected to influence this migration process. However, little is known about their roles in the zebrafish MFF. In this study, the function of PDGF and BMP signaling in zebrafish fin fold mesenchymal fibroblast migration was investigated using the chemical inhibitors PDGFR Tyrosine Kinase Inhibitor V and K02288 respectively. Transgenic lines Tg(-1.5hsp70l:smurf1-P2A-mCherry-CAAX,crybb:ECFP) and Tg(-1.5hsp70l:bmpr2bSF-P2A-mCherry-CAAX,crybb:ECFP) were also generated for the selective downregulation of canonical and LIMK1-mediated non-canonical BMP signaling respectively. PDGF signaling inhibition resulted in a supposed migration defect, reduction in fin fold mesenchymal fibroblast numbers, and increase in acridine orange-positive apoptotic cells within the fin mesoderm. BMP signaling inhibition resulted in a migration defect with a fall in fibroblast branching and cell area and an increase in cell aspect ratio. Taken together, our findings suggest a possible role for PDGF in the survival and/or migration of mesenchymal fibroblasts, and for BMP in mesenchymal fibroblast morphology and migration. |
| first_indexed | 2024-10-01T05:48:41Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/153141 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:48:41Z |
| publishDate | 2021 |
| publisher | Nanyang Technological University |
| record_format | dspace |
| spelling | ntu-10356/1531412023-02-28T18:08:32Z Integrating signaling pathways to pattern cells of the fin fold Ho, Charmaine Min Tom James Carney School of Biological Sciences tcarney@ntu.edu.sg Science::Biological sciences During zebrafish median fin fold (MFF) development, mesoderm-derived mesenchymal fibroblasts migrate outwards into the fin fold, towards the Apical Ectodermal Ridge (AER). The Platelet Derived Growth Factor (PDGF) and Bone Morphogenetic Protein (BMP) are small diffusible ligands expressed in the AER, with their corresponding receptors expressed in the fin mesenchyme, and are suspected to influence this migration process. However, little is known about their roles in the zebrafish MFF. In this study, the function of PDGF and BMP signaling in zebrafish fin fold mesenchymal fibroblast migration was investigated using the chemical inhibitors PDGFR Tyrosine Kinase Inhibitor V and K02288 respectively. Transgenic lines Tg(-1.5hsp70l:smurf1-P2A-mCherry-CAAX,crybb:ECFP) and Tg(-1.5hsp70l:bmpr2bSF-P2A-mCherry-CAAX,crybb:ECFP) were also generated for the selective downregulation of canonical and LIMK1-mediated non-canonical BMP signaling respectively. PDGF signaling inhibition resulted in a supposed migration defect, reduction in fin fold mesenchymal fibroblast numbers, and increase in acridine orange-positive apoptotic cells within the fin mesoderm. BMP signaling inhibition resulted in a migration defect with a fall in fibroblast branching and cell area and an increase in cell aspect ratio. Taken together, our findings suggest a possible role for PDGF in the survival and/or migration of mesenchymal fibroblasts, and for BMP in mesenchymal fibroblast morphology and migration. Bachelor of Science in Biological Sciences 2021-11-09T00:48:46Z 2021-11-09T00:48:46Z 2021 Final Year Project (FYP) Ho, C. M. (2021). Integrating signaling pathways to pattern cells of the fin fold. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/153141 https://hdl.handle.net/10356/153141 en application/pdf Nanyang Technological University |
| spellingShingle | Science::Biological sciences Ho, Charmaine Min Integrating signaling pathways to pattern cells of the fin fold |
| title | Integrating signaling pathways to pattern cells of the fin fold |
| title_full | Integrating signaling pathways to pattern cells of the fin fold |
| title_fullStr | Integrating signaling pathways to pattern cells of the fin fold |
| title_full_unstemmed | Integrating signaling pathways to pattern cells of the fin fold |
| title_short | Integrating signaling pathways to pattern cells of the fin fold |
| title_sort | integrating signaling pathways to pattern cells of the fin fold |
| topic | Science::Biological sciences |
| url | https://hdl.handle.net/10356/153141 |
| work_keys_str_mv | AT hocharmainemin integratingsignalingpathwaystopatterncellsofthefinfold |