An enzymatic method for harvesting functional melanosomes after keratin extraction – maximizing resource recovery from human hair
Hair contains about 80% keratins and 1–3% melanin packaged in melanosomes. Both of these are high-value and functional raw materials that have potential applications in wide-ranging fields. While keratin extraction has been widely refined, efficient methods of melanosome extraction are limited. The...
| Main Authors: | , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2021
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| Online Access: | https://hdl.handle.net/10356/153487 |
| _version_ | 1826115818453204992 |
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| author | Zhang, Nan Lai, Hui Ying Gautam, Archana Yu, Darien De Kwek Dong, Yibing Wang, Qiang Ng, Kee Woei |
| author2 | School of Materials Science and Engineering |
| author_facet | School of Materials Science and Engineering Zhang, Nan Lai, Hui Ying Gautam, Archana Yu, Darien De Kwek Dong, Yibing Wang, Qiang Ng, Kee Woei |
| author_sort | Zhang, Nan |
| collection | NTU |
| description | Hair contains about 80% keratins and 1–3% melanin packaged in melanosomes. Both of these are high-value and functional raw materials that have potential applications in wide-ranging fields. While keratin extraction has been widely refined, efficient methods of melanosome extraction are limited. The extraction of melanosomes requires complete removal of keratin, thus combining keratin extraction and melanosome isolation is logical. Herein, a successive process to harvest melanosomes after keratin extraction from human hair waste was developed. The yield of melanosomes was about 1.3% of the total hair mass. The structure of harvested melanosomes is well preserved based on surface morphology and interior ultrastructural observations using electron microscopy. The chemical structure, ultraviolet (UV)-filtering ability, and thermal stability of the melanosomes are examined to demonstrate preservation of native functions. Our strategy of combining melanosome isolation with keratin extraction is shown to be effective and significantly improves the total resource recovery efficiency from human hair waste. |
| first_indexed | 2024-10-01T04:01:23Z |
| format | Journal Article |
| id | ntu-10356/153487 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:01:23Z |
| publishDate | 2021 |
| record_format | dspace |
| spelling | ntu-10356/1534872022-01-01T20:11:50Z An enzymatic method for harvesting functional melanosomes after keratin extraction – maximizing resource recovery from human hair Zhang, Nan Lai, Hui Ying Gautam, Archana Yu, Darien De Kwek Dong, Yibing Wang, Qiang Ng, Kee Woei School of Materials Science and Engineering Nanyang Environment and Water Research Institute Engineering::Materials::Biomaterials Human Hair Resource Recovery Hair contains about 80% keratins and 1–3% melanin packaged in melanosomes. Both of these are high-value and functional raw materials that have potential applications in wide-ranging fields. While keratin extraction has been widely refined, efficient methods of melanosome extraction are limited. The extraction of melanosomes requires complete removal of keratin, thus combining keratin extraction and melanosome isolation is logical. Herein, a successive process to harvest melanosomes after keratin extraction from human hair waste was developed. The yield of melanosomes was about 1.3% of the total hair mass. The structure of harvested melanosomes is well preserved based on surface morphology and interior ultrastructural observations using electron microscopy. The chemical structure, ultraviolet (UV)-filtering ability, and thermal stability of the melanosomes are examined to demonstrate preservation of native functions. Our strategy of combining melanosome isolation with keratin extraction is shown to be effective and significantly improves the total resource recovery efficiency from human hair waste. Agency for Science, Technology and Research (A*STAR) Accepted version This research was supported by the Agency for Science, Technology and Research (A*STAR) under its Acne and Sebaceous Gland Program & Wound Care Innovation for the Tropics IAF-PP (Grant Nos. H17/01/a0/008 & H17/01/a0/0L9), the National Natural Science Foundation of China (Grant No. 51673087), and the China Scholarship Council (Grant No.201906790039 to N.Z.). 2021-12-12T06:01:39Z 2021-12-12T06:01:39Z 2021 Journal Article Zhang, N., Lai, H. Y., Gautam, A., Yu, D. D. K., Dong, Y., Wang, Q. & Ng, K. W. (2021). An enzymatic method for harvesting functional melanosomes after keratin extraction – maximizing resource recovery from human hair. Journal of Polymers and the Environment. https://dx.doi.org/10.1007/s10924-021-02246-8 1566-2543 https://hdl.handle.net/10356/153487 10.1007/s10924-021-02246-8 en H17/01/a0/008 H17/01/a0/0L9 NSF of China 51673087 CSC 201906790039 Journal of Polymers and the Environment © 2021 The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. This paper was published in Journal of Polymers and the Environment and is made available with permission of The Author(s). application/pdf |
| spellingShingle | Engineering::Materials::Biomaterials Human Hair Resource Recovery Zhang, Nan Lai, Hui Ying Gautam, Archana Yu, Darien De Kwek Dong, Yibing Wang, Qiang Ng, Kee Woei An enzymatic method for harvesting functional melanosomes after keratin extraction – maximizing resource recovery from human hair |
| title | An enzymatic method for harvesting functional melanosomes after keratin extraction – maximizing resource recovery from human hair |
| title_full | An enzymatic method for harvesting functional melanosomes after keratin extraction – maximizing resource recovery from human hair |
| title_fullStr | An enzymatic method for harvesting functional melanosomes after keratin extraction – maximizing resource recovery from human hair |
| title_full_unstemmed | An enzymatic method for harvesting functional melanosomes after keratin extraction – maximizing resource recovery from human hair |
| title_short | An enzymatic method for harvesting functional melanosomes after keratin extraction – maximizing resource recovery from human hair |
| title_sort | enzymatic method for harvesting functional melanosomes after keratin extraction maximizing resource recovery from human hair |
| topic | Engineering::Materials::Biomaterials Human Hair Resource Recovery |
| url | https://hdl.handle.net/10356/153487 |
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