Anticancer potential of L-histidine-capped silver nanoparticles against human cervical cancer cells (SIHA)
This study reports the synthesis of silver nanoparticles using amino acid L-histidine as a reducing and capping agent as an eco-friendly approach. Fabricated L-histidine-capped silver nanoparticles (L-HAgNPs) were characterized by spectroscopic and microscopic studies. Spherical shaped L-HAgNPs were...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2021
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| Online Access: | https://hdl.handle.net/10356/153510 |
| _version_ | 1811680012184584192 |
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| author | Mohammed Asik, Rajmohamed Manikkaraja, Chidhambaram Tamil Surya, Karuppusamy Suganthy, Natarajan Priya Aarthy, Archunan Mathe, Domokos Sivakumar, Muthusamy Archunan, Govindaraju Padmanabhan, Parasuraman Gulyás, Balázs |
| author2 | Lee Kong Chian School of Medicine (LKCMedicine) |
| author_facet | Lee Kong Chian School of Medicine (LKCMedicine) Mohammed Asik, Rajmohamed Manikkaraja, Chidhambaram Tamil Surya, Karuppusamy Suganthy, Natarajan Priya Aarthy, Archunan Mathe, Domokos Sivakumar, Muthusamy Archunan, Govindaraju Padmanabhan, Parasuraman Gulyás, Balázs |
| author_sort | Mohammed Asik, Rajmohamed |
| collection | NTU |
| description | This study reports the synthesis of silver nanoparticles using amino acid L-histidine as a reducing and capping agent as an eco-friendly approach. Fabricated L-histidine-capped silver nanoparticles (L-HAgNPs) were characterized by spectroscopic and microscopic studies. Spherical shaped L-HAgNPs were synthesized with a particle size of 47.43 ± 19.83 nm and zeta potential of -20.5 ± 0.95 mV. Results of the anticancer potential of L-HAgNPs showed antiproliferative effect against SiHa cells in a dose-dependent manner with an IC50 value of 18.25 ± 0.36 µg/mL. Fluorescent microscopic analysis revealed L-HAgNPs induced reactive oxygen species (ROS) mediated mitochondrial dysfunction, leading to activation of apoptotic pathway and DNA damage eventually causing cell death. To conclude, L-HAgNPs can act as promising candidates for cervical cancer therapy. |
| first_indexed | 2024-10-01T03:18:17Z |
| format | Journal Article |
| id | ntu-10356/153510 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:18:17Z |
| publishDate | 2021 |
| record_format | dspace |
| spelling | ntu-10356/1535102023-03-05T16:43:05Z Anticancer potential of L-histidine-capped silver nanoparticles against human cervical cancer cells (SIHA) Mohammed Asik, Rajmohamed Manikkaraja, Chidhambaram Tamil Surya, Karuppusamy Suganthy, Natarajan Priya Aarthy, Archunan Mathe, Domokos Sivakumar, Muthusamy Archunan, Govindaraju Padmanabhan, Parasuraman Gulyás, Balázs Lee Kong Chian School of Medicine (LKCMedicine) Cognitive Neuroimaging Centre Science::Medicine Anticancer Activity L-Histidine This study reports the synthesis of silver nanoparticles using amino acid L-histidine as a reducing and capping agent as an eco-friendly approach. Fabricated L-histidine-capped silver nanoparticles (L-HAgNPs) were characterized by spectroscopic and microscopic studies. Spherical shaped L-HAgNPs were synthesized with a particle size of 47.43 ± 19.83 nm and zeta potential of -20.5 ± 0.95 mV. Results of the anticancer potential of L-HAgNPs showed antiproliferative effect against SiHa cells in a dose-dependent manner with an IC50 value of 18.25 ± 0.36 µg/mL. Fluorescent microscopic analysis revealed L-HAgNPs induced reactive oxygen species (ROS) mediated mitochondrial dysfunction, leading to activation of apoptotic pathway and DNA damage eventually causing cell death. To conclude, L-HAgNPs can act as promising candidates for cervical cancer therapy. Nanyang Technological University Published version R.M.A. thanks Science and Engineering Research Board and Indian Council of Medical Research, New Delhi, India, for providing financial support through SERB—Overseas Visiting Doctoral Fellowship (ODF/2018/001044) and Senior Research Fellow (No. 2017-2853/SCRTBMS). N.S. thanks the University Grants Commission, New Delhi, India, for the financial support through the UGC-Start-Up Grant (Ref. No. F. 30-381/2017 (BSR), dated 6 July 2017) and RUSA—Phase 2.0 grant (No. F. 24-51/2014-U, Policy (TNMulti-Gen), Dept. of Edn. Govt. of India, dated 9 October 2018). D.M. thanks to European Union’s Horizon 2020 research and innovation program under grant agreement No 739593. HCEMM supported by EU Program: H2020-EU.4.a. This work was also partly funded by a grant from the Hungarian National Research, Development and Innovation Office (Thematic Excellence Program, TKP-BIOImaging, financed under the 2020-4.1.1-TKP2020 funding scheme). B.G. and P.P. thank Data Science and AI Research (DSAIR) Centre of NTU (Project Number ADH-11/2017-DSAIR), Imaging Probe Development Platform (IPDP), and the support from the Cognitive NeuroImaging Centre (CONIC) at NTU. G.A. acknowledges University Grants Commission, New Delhi, India, for the award of UGC-BSR Faculty Fellow (No. F.18-1/2011(BSR) dt. 4 January 2017). P.P. and B.G. acknowledge the support from Lee Kong Chian School of Medicine and Nanyang Technological University, Singapore. 2021-12-06T03:01:20Z 2021-12-06T03:01:20Z 2021 Journal Article Mohammed Asik, R., Manikkaraja, C., Tamil Surya, K., Suganthy, N., Priya Aarthy, A., Mathe, D., Sivakumar, M., Archunan, G., Padmanabhan, P. & Gulyás, B. (2021). Anticancer potential of L-histidine-capped silver nanoparticles against human cervical cancer cells (SIHA). Nanomaterials, 11(11), 3154-. https://dx.doi.org/10.3390/nano11113154 2079-4991 https://hdl.handle.net/10356/153510 10.3390/nano11113154 34835918 2-s2.0-85119612880 11 11 3154 en ADH-11/2017-DSAIR Nanomaterials © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). application/pdf |
| spellingShingle | Science::Medicine Anticancer Activity L-Histidine Mohammed Asik, Rajmohamed Manikkaraja, Chidhambaram Tamil Surya, Karuppusamy Suganthy, Natarajan Priya Aarthy, Archunan Mathe, Domokos Sivakumar, Muthusamy Archunan, Govindaraju Padmanabhan, Parasuraman Gulyás, Balázs Anticancer potential of L-histidine-capped silver nanoparticles against human cervical cancer cells (SIHA) |
| title | Anticancer potential of L-histidine-capped silver nanoparticles against human cervical cancer cells (SIHA) |
| title_full | Anticancer potential of L-histidine-capped silver nanoparticles against human cervical cancer cells (SIHA) |
| title_fullStr | Anticancer potential of L-histidine-capped silver nanoparticles against human cervical cancer cells (SIHA) |
| title_full_unstemmed | Anticancer potential of L-histidine-capped silver nanoparticles against human cervical cancer cells (SIHA) |
| title_short | Anticancer potential of L-histidine-capped silver nanoparticles against human cervical cancer cells (SIHA) |
| title_sort | anticancer potential of l histidine capped silver nanoparticles against human cervical cancer cells siha |
| topic | Science::Medicine Anticancer Activity L-Histidine |
| url | https://hdl.handle.net/10356/153510 |
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