Pharyngeal microbial signatures are predictive of the risk of fungal pneumonia in hematologic patients
The ability to predict invasive fungal infections (IFI) in patients with hematological malignancies is fundamental for successful therapy. Although gut dysbiosis is known to occur in hematological patients, whether airway dysbiosis also contributes to the risk of IFI has not been investigated. Nasal...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2021
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/153585 |
| _version_ | 1826111165437050880 |
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| author | Costantini, Claudio Nunzi, Emilia Spolzino, Angelica Palmieri, Melissa Renga, Giorgia Zelante, Teresa Englmaier, Lukas Coufalikova, Katerina Spáčil, Zdeněk Borghi, Monica Bellet, Marina M. Acerbi, Enzo Puccetti, Matteo Giovagnoli, Stefano Spaccapelo, Roberta Talesa, Vincenzo N. Lomurno, Giuseppe Merli, Francesco Facchini, Luca Spadea, Antonio Melillo, Lorella Codeluppi, Katia Marchesi, Francesco Marchesini, Gessica Valente, Daniela Dragonetti, Giulia Nadali, Gianpaolo Pagano, Livio Aversa, Franco Romani, Luigina |
| author2 | Singapore Centre for Environmental Life Sciences and Engineering |
| author_facet | Singapore Centre for Environmental Life Sciences and Engineering Costantini, Claudio Nunzi, Emilia Spolzino, Angelica Palmieri, Melissa Renga, Giorgia Zelante, Teresa Englmaier, Lukas Coufalikova, Katerina Spáčil, Zdeněk Borghi, Monica Bellet, Marina M. Acerbi, Enzo Puccetti, Matteo Giovagnoli, Stefano Spaccapelo, Roberta Talesa, Vincenzo N. Lomurno, Giuseppe Merli, Francesco Facchini, Luca Spadea, Antonio Melillo, Lorella Codeluppi, Katia Marchesi, Francesco Marchesini, Gessica Valente, Daniela Dragonetti, Giulia Nadali, Gianpaolo Pagano, Livio Aversa, Franco Romani, Luigina |
| author_sort | Costantini, Claudio |
| collection | NTU |
| description | The ability to predict invasive fungal infections (IFI) in patients with hematological malignancies is fundamental for successful therapy. Although gut dysbiosis is known to occur in hematological patients, whether airway dysbiosis also contributes to the risk of IFI has not been investigated. Nasal and oropharyngeal swabs were collected for functional microbiota characterization in 173 patients with hematological malignancies recruited in a multicenter, prospective, observational study and stratified according to the risk of developing IFI. A lower microbial richness and evenness were found in the pharyngeal microbiota of high-risk patients that were associated with a distinct taxonomic and metabolic profile. A murine model of IFI provided biologic plausibility for the finding that loss of protective anaerobes, such as Clostridiales and Bacteroidetes, along with an apparent restricted availability of tryptophan, is causally linked to the risk of IFI in hematologic patients and indicates avenues for antimicrobial stewardship and metabolic reequilibrium in IFI. |
| first_indexed | 2024-10-01T02:46:11Z |
| format | Journal Article |
| id | ntu-10356/153585 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:46:11Z |
| publishDate | 2021 |
| record_format | dspace |
| spelling | ntu-10356/1535852021-12-09T02:30:45Z Pharyngeal microbial signatures are predictive of the risk of fungal pneumonia in hematologic patients Costantini, Claudio Nunzi, Emilia Spolzino, Angelica Palmieri, Melissa Renga, Giorgia Zelante, Teresa Englmaier, Lukas Coufalikova, Katerina Spáčil, Zdeněk Borghi, Monica Bellet, Marina M. Acerbi, Enzo Puccetti, Matteo Giovagnoli, Stefano Spaccapelo, Roberta Talesa, Vincenzo N. Lomurno, Giuseppe Merli, Francesco Facchini, Luca Spadea, Antonio Melillo, Lorella Codeluppi, Katia Marchesi, Francesco Marchesini, Gessica Valente, Daniela Dragonetti, Giulia Nadali, Gianpaolo Pagano, Livio Aversa, Franco Romani, Luigina Singapore Centre for Environmental Life Sciences and Engineering Science::Biological sciences Hematological Malignancies Airway Microbiome The ability to predict invasive fungal infections (IFI) in patients with hematological malignancies is fundamental for successful therapy. Although gut dysbiosis is known to occur in hematological patients, whether airway dysbiosis also contributes to the risk of IFI has not been investigated. Nasal and oropharyngeal swabs were collected for functional microbiota characterization in 173 patients with hematological malignancies recruited in a multicenter, prospective, observational study and stratified according to the risk of developing IFI. A lower microbial richness and evenness were found in the pharyngeal microbiota of high-risk patients that were associated with a distinct taxonomic and metabolic profile. A murine model of IFI provided biologic plausibility for the finding that loss of protective anaerobes, such as Clostridiales and Bacteroidetes, along with an apparent restricted availability of tryptophan, is causally linked to the risk of IFI in hematologic patients and indicates avenues for antimicrobial stewardship and metabolic reequilibrium in IFI. This work was supported by FunMeta Project (ERC-2011-AdG 293714), MicroTher(ERC-2018-PoC-813099), and Gilead (IN-IT-131-4525-518872.9) to L.R. and the GrantAgency of the Czech Republic (GACR No 17-24592Y) and the Czech Ministry ofEducation, Youth and Sports (CETOCOEN PLUS CZ.02.1.01/0.0/0.0/15_003/0000469;LM2015051 and CETOCOEN EXCELLENCE Teaming 2 project CZ.02.1.01/0.0/0.0/18_046/0015975; and Horizon2020 project 857560) to Z.S. 2021-12-09T02:30:12Z 2021-12-09T02:30:12Z 2021 Journal Article Costantini, C., Nunzi, E., Spolzino, A., Palmieri, M., Renga, G., Zelante, T., Englmaier, L., Coufalikova, K., Spáčil, Z., Borghi, M., Bellet, M. M., Acerbi, E., Puccetti, M., Giovagnoli, S., Spaccapelo, R., Talesa, V. N., Lomurno, G., Merli, F., Facchini, L., ...Romani, L. (2021). Pharyngeal microbial signatures are predictive of the risk of fungal pneumonia in hematologic patients. Infection and Immunity, 89(8), e0010521-21-. https://dx.doi.org/10.1128/IAI.00105-21 0019-9567 https://hdl.handle.net/10356/153585 10.1128/IAI.00105-21 33782152 2-s2.0-85111079461 8 89 e0010521-21 en Infection and Immunity © 2021 American Society for Microbiology. All Rights Reserved. |
| spellingShingle | Science::Biological sciences Hematological Malignancies Airway Microbiome Costantini, Claudio Nunzi, Emilia Spolzino, Angelica Palmieri, Melissa Renga, Giorgia Zelante, Teresa Englmaier, Lukas Coufalikova, Katerina Spáčil, Zdeněk Borghi, Monica Bellet, Marina M. Acerbi, Enzo Puccetti, Matteo Giovagnoli, Stefano Spaccapelo, Roberta Talesa, Vincenzo N. Lomurno, Giuseppe Merli, Francesco Facchini, Luca Spadea, Antonio Melillo, Lorella Codeluppi, Katia Marchesi, Francesco Marchesini, Gessica Valente, Daniela Dragonetti, Giulia Nadali, Gianpaolo Pagano, Livio Aversa, Franco Romani, Luigina Pharyngeal microbial signatures are predictive of the risk of fungal pneumonia in hematologic patients |
| title | Pharyngeal microbial signatures are predictive of the risk of fungal pneumonia in hematologic patients |
| title_full | Pharyngeal microbial signatures are predictive of the risk of fungal pneumonia in hematologic patients |
| title_fullStr | Pharyngeal microbial signatures are predictive of the risk of fungal pneumonia in hematologic patients |
| title_full_unstemmed | Pharyngeal microbial signatures are predictive of the risk of fungal pneumonia in hematologic patients |
| title_short | Pharyngeal microbial signatures are predictive of the risk of fungal pneumonia in hematologic patients |
| title_sort | pharyngeal microbial signatures are predictive of the risk of fungal pneumonia in hematologic patients |
| topic | Science::Biological sciences Hematological Malignancies Airway Microbiome |
| url | https://hdl.handle.net/10356/153585 |
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