Bio-magnetic nanoparticles based electrochemical immunosensor
This thesis explores the development of a novel disposable immunosensor based on Fe2O3 core with carbon paste and gold coating. The project explores the preparation, characterisation and measurement of the various properties of the immunosensor which will be helpful in the detection of Prostate-Spec...
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| Format: | Final Year Project (FYP) |
| Language: | English |
| Published: |
2009
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| Online Access: | http://hdl.handle.net/10356/15377 |
| _version_ | 1826125951449169920 |
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| author | Shazzharif Shamsudin. |
| author2 | Raju Vijayaraghavan Ramanujan |
| author_facet | Raju Vijayaraghavan Ramanujan Shazzharif Shamsudin. |
| author_sort | Shazzharif Shamsudin. |
| collection | NTU |
| description | This thesis explores the development of a novel disposable immunosensor based on Fe2O3 core with carbon paste and gold coating. The project explores the preparation, characterisation and measurement of the various properties of the immunosensor which will be helpful in the detection of Prostate-Specific Antigen (PSA). The detection of PSA is done by an amperometric route with a constant voltage of -300mV vs. Saturated Calomel Electrode (SCE).
The combination of Fe2O3 core, carbon paste and gold coating showed good adsorption properties for the anti-PSA (antibody) and yielded very good results. 5μl of 5 mg/ml anti-PSA is immobilised onto the electrode by physical adsorption for a time period of 24 hours overnight at 4oC. The electrode was tested against antigen at concentrations of 0.25 mg/L, 0.5 mg/L and 0.75 mg/L in pH 7.4 Phosphate Buffer Saline (0.1M). It was observed that the current increased by a substantial value of 3 orders, and this presents us with a good electrochemical response for the detection of PSA. This methodology can thus be proposed for use in clinical applications and even be used for detection of other antigens. |
| first_indexed | 2024-10-01T06:44:47Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/15377 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:44:47Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | ntu-10356/153772023-03-04T15:38:44Z Bio-magnetic nanoparticles based electrochemical immunosensor Shazzharif Shamsudin. Raju Vijayaraghavan Ramanujan School of Materials Science and Engineering DRNTU::Engineering::Nanotechnology DRNTU::Engineering::Materials::Magnetic materials DRNTU::Engineering::Materials::Biomaterials This thesis explores the development of a novel disposable immunosensor based on Fe2O3 core with carbon paste and gold coating. The project explores the preparation, characterisation and measurement of the various properties of the immunosensor which will be helpful in the detection of Prostate-Specific Antigen (PSA). The detection of PSA is done by an amperometric route with a constant voltage of -300mV vs. Saturated Calomel Electrode (SCE). The combination of Fe2O3 core, carbon paste and gold coating showed good adsorption properties for the anti-PSA (antibody) and yielded very good results. 5μl of 5 mg/ml anti-PSA is immobilised onto the electrode by physical adsorption for a time period of 24 hours overnight at 4oC. The electrode was tested against antigen at concentrations of 0.25 mg/L, 0.5 mg/L and 0.75 mg/L in pH 7.4 Phosphate Buffer Saline (0.1M). It was observed that the current increased by a substantial value of 3 orders, and this presents us with a good electrochemical response for the detection of PSA. This methodology can thus be proposed for use in clinical applications and even be used for detection of other antigens. Bachelor of Engineering (Materials Engineering) 2009-04-28T02:20:28Z 2009-04-28T02:20:28Z 2008 2008 Final Year Project (FYP) http://hdl.handle.net/10356/15377 en 51 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Nanotechnology DRNTU::Engineering::Materials::Magnetic materials DRNTU::Engineering::Materials::Biomaterials Shazzharif Shamsudin. Bio-magnetic nanoparticles based electrochemical immunosensor |
| title | Bio-magnetic nanoparticles based electrochemical immunosensor |
| title_full | Bio-magnetic nanoparticles based electrochemical immunosensor |
| title_fullStr | Bio-magnetic nanoparticles based electrochemical immunosensor |
| title_full_unstemmed | Bio-magnetic nanoparticles based electrochemical immunosensor |
| title_short | Bio-magnetic nanoparticles based electrochemical immunosensor |
| title_sort | bio magnetic nanoparticles based electrochemical immunosensor |
| topic | DRNTU::Engineering::Nanotechnology DRNTU::Engineering::Materials::Magnetic materials DRNTU::Engineering::Materials::Biomaterials |
| url | http://hdl.handle.net/10356/15377 |
| work_keys_str_mv | AT shazzharifshamsudin biomagneticnanoparticlesbasedelectrochemicalimmunosensor |