Improved cell viability for large-scale biofabrication with photo-crosslinkable hydrogel systems through a dual-photoinitiator approach
Biofabrication with various hydrogel systems allows the production of tissue or organ constructs in vitro to address various challenges in healthcare and medicine. In particular, photocrosslinkable hydrogels have great advantages such as excellent spatial and temporal selectivity and low processing...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2022
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| Online Access: | https://hdl.handle.net/10356/154920 |
| _version_ | 1811683354705133568 |
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| author | Han, Win Tun Jang, Taesik Chen, Shengyang Chong, Lydia Shi Hui Jung, Hyun-Do Song, Juha |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Han, Win Tun Jang, Taesik Chen, Shengyang Chong, Lydia Shi Hui Jung, Hyun-Do Song, Juha |
| author_sort | Han, Win Tun |
| collection | NTU |
| description | Biofabrication with various hydrogel systems allows the production of tissue or organ constructs in vitro to address various challenges in healthcare and medicine. In particular, photocrosslinkable hydrogels have great advantages such as excellent spatial and temporal selectivity and low processing cost and energy requirements. However, inefficient polymerization kinetics of commercialized photoinitiators upon exposure to UV-A radiation or visible light increase processing time, often compromising cell viability. In this study, we developed a hydrogel crosslinking system which exhibited efficient crosslinking properties and desired mechanical properties with high cell viability, through a dual-photoinitiator approach. Through the co-existence of Irgacure 2959 and VA-086, the overall crosslinking process was completed with a minimal UV dosage during a significantly reduced crosslinking time, producing mechanically robust hydrogel constructs, while most encapsulated cells within the hydrogel constructs remained viable. Moreover, we fabricated a large PEGDA hydrogel construct with a single microchannel as a proof of concept for hydrogels with vasculature to demonstrate the versatility of the system. Our dual-photoinitiator approach allowed the production of this photocrosslinkable hydrogel system with microchannels, significantly improving cell viability and processing efficiency, yet maintaining good mechanical stability. Taken together, we envision the concurrent use of photoinitiators, Irgacure 2959 and VA-086, opening potential avenues for the utilization of various photocrosslinkable hydrogel systems in perfusable large artificial tissue for in vivo and ex vivo applications with improved processing efficiency and cell viability. |
| first_indexed | 2024-10-01T04:11:24Z |
| format | Journal Article |
| id | ntu-10356/154920 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:11:24Z |
| publishDate | 2022 |
| record_format | dspace |
| spelling | ntu-10356/1549202022-01-14T07:15:31Z Improved cell viability for large-scale biofabrication with photo-crosslinkable hydrogel systems through a dual-photoinitiator approach Han, Win Tun Jang, Taesik Chen, Shengyang Chong, Lydia Shi Hui Jung, Hyun-Do Song, Juha School of Chemical and Biomedical Engineering Engineering::Chemical engineering Tissue-Engineered Constructs Photopolymerizable Hydrogels Biofabrication with various hydrogel systems allows the production of tissue or organ constructs in vitro to address various challenges in healthcare and medicine. In particular, photocrosslinkable hydrogels have great advantages such as excellent spatial and temporal selectivity and low processing cost and energy requirements. However, inefficient polymerization kinetics of commercialized photoinitiators upon exposure to UV-A radiation or visible light increase processing time, often compromising cell viability. In this study, we developed a hydrogel crosslinking system which exhibited efficient crosslinking properties and desired mechanical properties with high cell viability, through a dual-photoinitiator approach. Through the co-existence of Irgacure 2959 and VA-086, the overall crosslinking process was completed with a minimal UV dosage during a significantly reduced crosslinking time, producing mechanically robust hydrogel constructs, while most encapsulated cells within the hydrogel constructs remained viable. Moreover, we fabricated a large PEGDA hydrogel construct with a single microchannel as a proof of concept for hydrogels with vasculature to demonstrate the versatility of the system. Our dual-photoinitiator approach allowed the production of this photocrosslinkable hydrogel system with microchannels, significantly improving cell viability and processing efficiency, yet maintaining good mechanical stability. Taken together, we envision the concurrent use of photoinitiators, Irgacure 2959 and VA-086, opening potential avenues for the utilization of various photocrosslinkable hydrogel systems in perfusable large artificial tissue for in vivo and ex vivo applications with improved processing efficiency and cell viability. Agency for Science, Technology and Research (A*STAR) Ministry of Education (MOE) This research was supported by the AcRF Tier 1 grant 2017-T1- 001-246 (RG51/17) from the Ministry of Education of Singapore, and the A*STAR AME IRG grant A1983c0031 from A*STAR. 2022-01-14T07:15:31Z 2022-01-14T07:15:31Z 2019 Journal Article Han, W. T., Jang, T., Chen, S., Chong, L. S. H., Jung, H. & Song, J. (2019). Improved cell viability for large-scale biofabrication with photo-crosslinkable hydrogel systems through a dual-photoinitiator approach. Biomaterials Science, 8(1), 450-461. https://dx.doi.org/10.1039/c9bm01347d 2047-4849 https://hdl.handle.net/10356/154920 10.1039/c9bm01347d 31748767 2-s2.0-85076875560 1 8 450 461 en 2017-T1- 001-246 (RG51/17) A1983c0031 Biomaterials Science © The Royal Society of Chemistry 2020. All rights reserved. |
| spellingShingle | Engineering::Chemical engineering Tissue-Engineered Constructs Photopolymerizable Hydrogels Han, Win Tun Jang, Taesik Chen, Shengyang Chong, Lydia Shi Hui Jung, Hyun-Do Song, Juha Improved cell viability for large-scale biofabrication with photo-crosslinkable hydrogel systems through a dual-photoinitiator approach |
| title | Improved cell viability for large-scale biofabrication with photo-crosslinkable hydrogel systems through a dual-photoinitiator approach |
| title_full | Improved cell viability for large-scale biofabrication with photo-crosslinkable hydrogel systems through a dual-photoinitiator approach |
| title_fullStr | Improved cell viability for large-scale biofabrication with photo-crosslinkable hydrogel systems through a dual-photoinitiator approach |
| title_full_unstemmed | Improved cell viability for large-scale biofabrication with photo-crosslinkable hydrogel systems through a dual-photoinitiator approach |
| title_short | Improved cell viability for large-scale biofabrication with photo-crosslinkable hydrogel systems through a dual-photoinitiator approach |
| title_sort | improved cell viability for large scale biofabrication with photo crosslinkable hydrogel systems through a dual photoinitiator approach |
| topic | Engineering::Chemical engineering Tissue-Engineered Constructs Photopolymerizable Hydrogels |
| url | https://hdl.handle.net/10356/154920 |
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