Evaluating the antioxidant effects of human hair protein extracts
The intrinsically high cysteine content in human hair keratins and keratin associated proteins confer hair its outstanding mechanical strength through the formation of strong intermolecular disulfide bonds. In addition, these proteins offer the potential to be exploited as potent antioxidants. This...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2022
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| Online Access: | https://hdl.handle.net/10356/155073 |
| _version_ | 1826127163011629056 |
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| author | Lai, Hui Ying Wang, Shuai Singh, Vaishali Nguyen, Luong T. H. Ng, Kee Woei |
| author2 | School of Materials Science and Engineering |
| author_facet | School of Materials Science and Engineering Lai, Hui Ying Wang, Shuai Singh, Vaishali Nguyen, Luong T. H. Ng, Kee Woei |
| author_sort | Lai, Hui Ying |
| collection | NTU |
| description | The intrinsically high cysteine content in human hair keratins and keratin associated proteins confer hair its outstanding mechanical strength through the formation of strong intermolecular disulfide bonds. In addition, these proteins offer the potential to be exploited as potent antioxidants. This report presents our findings on the antioxidant effects of human hair protein extracts and their consequent protective role against oxidative stress in human dermal fibroblast (HDF) cultures. Protein extracts were obtained from human hair using sodium sulfide as the reducing agent, and characterized using SDS-PAGE, Western blotting, MALDI-ToF mass spectrometry and amino acid analysis. Cysteine was found to account for 11.2 mol % in the extracted fractions. By measuring 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radical scavenging activity, the hair protein fractions were shown to possess significant antioxidant ability (IC50 = 16.22 μM). As a supplement in cell culture media, the extracts protected HDFs from H2O2 induced oxidative stress, which was demonstrated by the maintenance of cell viability and reduced reactive oxygen species production. Besides offering mechanical support as a scaffolding material, the unique antioxidizing ability of human hair protein extracts may also be exploited in biomedical applications. |
| first_indexed | 2024-10-01T07:04:16Z |
| format | Journal Article |
| id | ntu-10356/155073 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:04:16Z |
| publishDate | 2022 |
| record_format | dspace |
| spelling | ntu-10356/1550732022-02-05T20:11:23Z Evaluating the antioxidant effects of human hair protein extracts Lai, Hui Ying Wang, Shuai Singh, Vaishali Nguyen, Luong T. H. Ng, Kee Woei School of Materials Science and Engineering Interdisciplinary Graduate School (IGS) Nanyang Environment and Water Research Institute Environmental Chemistry and Materials Centre Engineering::Materials::Biomaterials Keratin Keratin Associated Proteins Antioxidant Biomaterial Human Hair Fibroblast The intrinsically high cysteine content in human hair keratins and keratin associated proteins confer hair its outstanding mechanical strength through the formation of strong intermolecular disulfide bonds. In addition, these proteins offer the potential to be exploited as potent antioxidants. This report presents our findings on the antioxidant effects of human hair protein extracts and their consequent protective role against oxidative stress in human dermal fibroblast (HDF) cultures. Protein extracts were obtained from human hair using sodium sulfide as the reducing agent, and characterized using SDS-PAGE, Western blotting, MALDI-ToF mass spectrometry and amino acid analysis. Cysteine was found to account for 11.2 mol % in the extracted fractions. By measuring 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radical scavenging activity, the hair protein fractions were shown to possess significant antioxidant ability (IC50 = 16.22 μM). As a supplement in cell culture media, the extracts protected HDFs from H2O2 induced oxidative stress, which was demonstrated by the maintenance of cell viability and reduced reactive oxygen species production. Besides offering mechanical support as a scaffolding material, the unique antioxidizing ability of human hair protein extracts may also be exploited in biomedical applications. Agency for Science, Technology and Research (A*STAR) Ministry of Education (MOE) Accepted version This work was supported by the Ministry of Education, AcRF-Tier 1 (RG42/13) and A*STAR-NTU-NHG 2nd Skin Research Grant (SRG/15035). 2022-02-04T07:47:15Z 2022-02-04T07:47:15Z 2018 Journal Article Lai, H. Y., Wang, S., Singh, V., Nguyen, L. T. H. & Ng, K. W. (2018). Evaluating the antioxidant effects of human hair protein extracts. Journal of Biomaterials Science, Polymer Edition, 29(7-9), 1081-1093. https://dx.doi.org/10.1080/09205063.2017.1421345 0920-5063 https://hdl.handle.net/10356/155073 10.1080/09205063.2017.1421345 29285991 2-s2.0-85040985245 7-9 29 1081 1093 en RG42/13 SRG/15035 Journal of Biomaterials Science, Polymer Edition This is an Accepted Manuscript of an article published by Taylor & Francis in Journal of Biomaterials Science, Polymer Edition on 03 Jan 2018, available online: http://www.tandfonline.com/10.1080/09205063.2017.1421345. application/pdf |
| spellingShingle | Engineering::Materials::Biomaterials Keratin Keratin Associated Proteins Antioxidant Biomaterial Human Hair Fibroblast Lai, Hui Ying Wang, Shuai Singh, Vaishali Nguyen, Luong T. H. Ng, Kee Woei Evaluating the antioxidant effects of human hair protein extracts |
| title | Evaluating the antioxidant effects of human hair protein extracts |
| title_full | Evaluating the antioxidant effects of human hair protein extracts |
| title_fullStr | Evaluating the antioxidant effects of human hair protein extracts |
| title_full_unstemmed | Evaluating the antioxidant effects of human hair protein extracts |
| title_short | Evaluating the antioxidant effects of human hair protein extracts |
| title_sort | evaluating the antioxidant effects of human hair protein extracts |
| topic | Engineering::Materials::Biomaterials Keratin Keratin Associated Proteins Antioxidant Biomaterial Human Hair Fibroblast |
| url | https://hdl.handle.net/10356/155073 |
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