Phosphatase POPX2 interferes with cell cycle by interacting with Chk1
Protein-protein interaction network analysis plays critical roles in predicting the functions of target proteins. In this study, we used a combination of SILAC-MS proteomics and bioinformatic approaches to identify Checkpoint Kinase 1 (Chk1) as a possible POPX2 phosphatase interacting protein. POPX2...
| Main Authors: | , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2022
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/155312 |
| _version_ | 1826121584941727744 |
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| author | Kim, Pu Rum Koon, Yen Ling Lee, Raphael Tze Chuen Azizan, Farouq Koh, Dylan Hong Zheng Chiam, Keng-Hwee Koh, Cheng-Gee |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Kim, Pu Rum Koon, Yen Ling Lee, Raphael Tze Chuen Azizan, Farouq Koh, Dylan Hong Zheng Chiam, Keng-Hwee Koh, Cheng-Gee |
| author_sort | Kim, Pu Rum |
| collection | NTU |
| description | Protein-protein interaction network analysis plays critical roles in predicting the functions of target proteins. In this study, we used a combination of SILAC-MS proteomics and bioinformatic approaches to identify Checkpoint Kinase 1 (Chk1) as a possible POPX2 phosphatase interacting protein. POPX2 is a PP2C phosphatase that has been implicated in cancer cell invasion and migration. From the Domain-Domain Interaction (DDI) database, we first determined that the PP2C phosphatase domain interacts with Pkinase domain. Subsequently, 46 proteins with Pkinase domain were identified from POPX2 SILAC-MS data. We then narrowed down the leads and confirmed the biological interaction between Chk1 and POPX2. We also found that Chk1 is a substrate of POPX2. Chk1 is a key regulator of the cell cycle and is activated when the cell suffers DNA damage. Our approach has led us to identify POPX2 as a regulator of Chk1 and can interfere with the normal function of Chk1 at G1-S transition of the cell cycle in response to DNA damage. |
| first_indexed | 2024-10-01T05:34:37Z |
| format | Journal Article |
| id | ntu-10356/155312 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:34:37Z |
| publishDate | 2022 |
| record_format | dspace |
| spelling | ntu-10356/1553122022-03-18T02:01:45Z Phosphatase POPX2 interferes with cell cycle by interacting with Chk1 Kim, Pu Rum Koon, Yen Ling Lee, Raphael Tze Chuen Azizan, Farouq Koh, Dylan Hong Zheng Chiam, Keng-Hwee Koh, Cheng-Gee School of Biological Sciences Interdisciplinary Graduate School (IGS) Bioinformatics Institute, A*STAR Science::Biological sciences Protein–Protein Interactions Partner of PIX 2 Phosphatase Protein-protein interaction network analysis plays critical roles in predicting the functions of target proteins. In this study, we used a combination of SILAC-MS proteomics and bioinformatic approaches to identify Checkpoint Kinase 1 (Chk1) as a possible POPX2 phosphatase interacting protein. POPX2 is a PP2C phosphatase that has been implicated in cancer cell invasion and migration. From the Domain-Domain Interaction (DDI) database, we first determined that the PP2C phosphatase domain interacts with Pkinase domain. Subsequently, 46 proteins with Pkinase domain were identified from POPX2 SILAC-MS data. We then narrowed down the leads and confirmed the biological interaction between Chk1 and POPX2. We also found that Chk1 is a substrate of POPX2. Chk1 is a key regulator of the cell cycle and is activated when the cell suffers DNA damage. Our approach has led us to identify POPX2 as a regulator of Chk1 and can interfere with the normal function of Chk1 at G1-S transition of the cell cycle in response to DNA damage. Ministry of Education (MOE) Nanyang Technological University This work was supported by the Nanyang Technological University [SUG]; Ministry of Education (Singapore) [2016- T1-002-081]. 2022-03-18T02:01:45Z 2022-03-18T02:01:45Z 2020 Journal Article Kim, P. R., Koon, Y. L., Lee, R. T. C., Azizan, F., Koh, D. H. Z., Chiam, K. & Koh, C. (2020). Phosphatase POPX2 interferes with cell cycle by interacting with Chk1. Cell Cycle, 19(4), 405-418. https://dx.doi.org/10.1080/15384101.2020.1711577 1538-4101 https://hdl.handle.net/10356/155312 10.1080/15384101.2020.1711577 31944151 2-s2.0-85078612986 4 19 405 418 en 2016- T1-002-081 Cell Cycle © 2020 Informa UK Limited, trading as Taylor & Francis Group. All rights reserved. |
| spellingShingle | Science::Biological sciences Protein–Protein Interactions Partner of PIX 2 Phosphatase Kim, Pu Rum Koon, Yen Ling Lee, Raphael Tze Chuen Azizan, Farouq Koh, Dylan Hong Zheng Chiam, Keng-Hwee Koh, Cheng-Gee Phosphatase POPX2 interferes with cell cycle by interacting with Chk1 |
| title | Phosphatase POPX2 interferes with cell cycle by interacting with Chk1 |
| title_full | Phosphatase POPX2 interferes with cell cycle by interacting with Chk1 |
| title_fullStr | Phosphatase POPX2 interferes with cell cycle by interacting with Chk1 |
| title_full_unstemmed | Phosphatase POPX2 interferes with cell cycle by interacting with Chk1 |
| title_short | Phosphatase POPX2 interferes with cell cycle by interacting with Chk1 |
| title_sort | phosphatase popx2 interferes with cell cycle by interacting with chk1 |
| topic | Science::Biological sciences Protein–Protein Interactions Partner of PIX 2 Phosphatase |
| url | https://hdl.handle.net/10356/155312 |
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