Development of a mechanically stable human hair keratin film for cell culture
An easy-to-handle keratin film was successfully fabricated using solely purified hair keratins. Keratin was extracted from human hair by an existing protocol. The extracted keratin was made into a mechanically stable film by solution casting and air-drying at room temperature. The films obtained wer...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2022
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/155466 |
| _version_ | 1811685873152950272 |
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| author | Tan, Bee Yi Nguyen, Luong T. H. Ng, Kee Woei |
| author2 | School of Materials Science and Engineering |
| author_facet | School of Materials Science and Engineering Tan, Bee Yi Nguyen, Luong T. H. Ng, Kee Woei |
| author_sort | Tan, Bee Yi |
| collection | NTU |
| description | An easy-to-handle keratin film was successfully fabricated using solely purified hair keratins. Keratin was extracted from human hair by an existing protocol. The extracted keratin was made into a mechanically stable film by solution casting and air-drying at room temperature. The films obtained were characterized for surface morphology, wettability, protein secondary structures, mechanical properties, permeability, and thermal properties. Interestingly, the keratin film showed distinct surface and cross-sectional morphology, and protein secondary structure transformation. In addition, the keratin film exhibited Young's modulus of 1.05 ± 0.09 GPa when it was dry. In the wet state, the keratin film behaved as viscoelastic material and was highly stretchable at 179 ± 17% strain at break. Permeability test was conducted using 20 kDa-FITC dextran which revealed an anomalous diffusion mechanism through the keratin film. Additionally, the keratin film elicited positive cellular responses by human epidermal keratinocytes (HEKs) in terms of enhanced cell proliferation, viability, keratin 14 expression, and IL-1α secretion, in comparison to collagen I. Taken together, a human hair keratin-based film with its mechanical and thermal stability, and cytocompatibility, presents a promising platform for cell culture applications. |
| first_indexed | 2024-10-01T04:51:26Z |
| format | Journal Article |
| id | ntu-10356/155466 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:51:26Z |
| publishDate | 2022 |
| record_format | dspace |
| spelling | ntu-10356/1554662022-03-05T20:11:23Z Development of a mechanically stable human hair keratin film for cell culture Tan, Bee Yi Nguyen, Luong T. H. Ng, Kee Woei School of Materials Science and Engineering Nanyang Environment and Water Research Institute Environmental Chemistry and Materials Centre Engineering::Materials::Biomaterials Human Hair Keratins Protein Film Stretchable Cell Carrier An easy-to-handle keratin film was successfully fabricated using solely purified hair keratins. Keratin was extracted from human hair by an existing protocol. The extracted keratin was made into a mechanically stable film by solution casting and air-drying at room temperature. The films obtained were characterized for surface morphology, wettability, protein secondary structures, mechanical properties, permeability, and thermal properties. Interestingly, the keratin film showed distinct surface and cross-sectional morphology, and protein secondary structure transformation. In addition, the keratin film exhibited Young's modulus of 1.05 ± 0.09 GPa when it was dry. In the wet state, the keratin film behaved as viscoelastic material and was highly stretchable at 179 ± 17% strain at break. Permeability test was conducted using 20 kDa-FITC dextran which revealed an anomalous diffusion mechanism through the keratin film. Additionally, the keratin film elicited positive cellular responses by human epidermal keratinocytes (HEKs) in terms of enhanced cell proliferation, viability, keratin 14 expression, and IL-1α secretion, in comparison to collagen I. Taken together, a human hair keratin-based film with its mechanical and thermal stability, and cytocompatibility, presents a promising platform for cell culture applications. Agency for Science, Technology and Research (A*STAR) Accepted version This research is supported by the Agency for Science, Technology and Research (A*STAR) under its Wound Care Innovation for the Tropics, IAF-PP (H17/01/a0/0L9). 2022-03-02T02:15:03Z 2022-03-02T02:15:03Z 2022 Journal Article Tan, B. Y., Nguyen, L. T. H. & Ng, K. W. (2022). Development of a mechanically stable human hair keratin film for cell culture. Materials Today Communications, 30, 103049-. https://dx.doi.org/10.1016/j.mtcomm.2021.103049 23524928 https://hdl.handle.net/10356/155466 10.1016/j.mtcomm.2021.103049 2-s2.0-85120653857 30 103049 en H17/01/a0/0L9 Materials Today Communications © 2021 Elsevier Ltd. All rights reserved. This paper was published in Materials Today Communications and is made available with permission of Elsevier Ltd. application/pdf |
| spellingShingle | Engineering::Materials::Biomaterials Human Hair Keratins Protein Film Stretchable Cell Carrier Tan, Bee Yi Nguyen, Luong T. H. Ng, Kee Woei Development of a mechanically stable human hair keratin film for cell culture |
| title | Development of a mechanically stable human hair keratin film for cell culture |
| title_full | Development of a mechanically stable human hair keratin film for cell culture |
| title_fullStr | Development of a mechanically stable human hair keratin film for cell culture |
| title_full_unstemmed | Development of a mechanically stable human hair keratin film for cell culture |
| title_short | Development of a mechanically stable human hair keratin film for cell culture |
| title_sort | development of a mechanically stable human hair keratin film for cell culture |
| topic | Engineering::Materials::Biomaterials Human Hair Keratins Protein Film Stretchable Cell Carrier |
| url | https://hdl.handle.net/10356/155466 |
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