Method development for quantitative analysis of thyroid hormones by LC-MS/MS.
Dessicated thyroid powder was sometimes adulterated in slimming supplements, resulting in serious safety and quality concerns. The complex sample matrix present in these slimming supplements also makes the screening and analysis for illegal drugs difficult. In this project, a LC-MS/MS method was dev...
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| Format: | Final Year Project (FYP) |
| Language: | English |
| Published: |
2009
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| Online Access: | http://hdl.handle.net/10356/15551 |
| _version_ | 1826128527889530880 |
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| author | Ng, Joseph Teck Lay. |
| author2 | Zeng Yun |
| author_facet | Zeng Yun Ng, Joseph Teck Lay. |
| author_sort | Ng, Joseph Teck Lay. |
| collection | NTU |
| description | Dessicated thyroid powder was sometimes adulterated in slimming supplements, resulting in serious safety and quality concerns. The complex sample matrix present in these slimming supplements also makes the screening and analysis for illegal drugs difficult. In this project, a LC-MS/MS method was developed to increase the recovery of thyroid hormone adulterated in slimming supplements. When spiked samples were pre-cleaned with ACN, subjected to 28 hours incubation and evaluated with LC-MS/MS in mobile phase [A] 0.2% formic acid and 0.2% of 1M ammonium formate in ultrapure water and [B] 0.2% formic acid and 0.2% of 1M ammonium formate in ACN, a moderate recovery of about 50 60% for T3 and a high recovery of 60 84% for T4 can be achieved using standard addition calibration. Furthermore, preliminary result from solid phase extraction showed that it is effective in minimizing ion suppression and has the potential to achieve a high recovery with proper optimization. |
| first_indexed | 2024-10-01T07:26:24Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/15551 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:26:24Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | ntu-10356/155512023-02-28T18:00:02Z Method development for quantitative analysis of thyroid hormones by LC-MS/MS. Ng, Joseph Teck Lay. Zeng Yun School of Biological Sciences DRNTU::Engineering::Chemical engineering::Biotechnology Dessicated thyroid powder was sometimes adulterated in slimming supplements, resulting in serious safety and quality concerns. The complex sample matrix present in these slimming supplements also makes the screening and analysis for illegal drugs difficult. In this project, a LC-MS/MS method was developed to increase the recovery of thyroid hormone adulterated in slimming supplements. When spiked samples were pre-cleaned with ACN, subjected to 28 hours incubation and evaluated with LC-MS/MS in mobile phase [A] 0.2% formic acid and 0.2% of 1M ammonium formate in ultrapure water and [B] 0.2% formic acid and 0.2% of 1M ammonium formate in ACN, a moderate recovery of about 50 60% for T3 and a high recovery of 60 84% for T4 can be achieved using standard addition calibration. Furthermore, preliminary result from solid phase extraction showed that it is effective in minimizing ion suppression and has the potential to achieve a high recovery with proper optimization. Bachelor of Science in Biological Sciences 2009-05-12T07:39:45Z 2009-05-12T07:39:45Z 2009 2009 Final Year Project (FYP) http://hdl.handle.net/10356/15551 en Nanyang Technological University 43 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Chemical engineering::Biotechnology Ng, Joseph Teck Lay. Method development for quantitative analysis of thyroid hormones by LC-MS/MS. |
| title | Method development for quantitative analysis of thyroid hormones by LC-MS/MS. |
| title_full | Method development for quantitative analysis of thyroid hormones by LC-MS/MS. |
| title_fullStr | Method development for quantitative analysis of thyroid hormones by LC-MS/MS. |
| title_full_unstemmed | Method development for quantitative analysis of thyroid hormones by LC-MS/MS. |
| title_short | Method development for quantitative analysis of thyroid hormones by LC-MS/MS. |
| title_sort | method development for quantitative analysis of thyroid hormones by lc ms ms |
| topic | DRNTU::Engineering::Chemical engineering::Biotechnology |
| url | http://hdl.handle.net/10356/15551 |
| work_keys_str_mv | AT ngjosephtecklay methoddevelopmentforquantitativeanalysisofthyroidhormonesbylcmsms |