Sunflower WRINKLED1 plays a key role in transcriptional regulation of oil biosynthesis
Sunflower (Helianthus annuus) is one of the most important oilseed crops worldwide. However, the transcriptional regulation underlying oil accumulation in sunflower is not fully understood. WRINKLED1 (WRI1) is an essential transcription factor governing oil accumulation in plant cells. Here, we iden...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2022
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/155728 |
| _version_ | 1811688311003021312 |
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| author | Lim, Audrey R. Q. Kong, Que Singh, Sanjay K. Guo, Liang Yuan, Ling Ma, Wei |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Lim, Audrey R. Q. Kong, Que Singh, Sanjay K. Guo, Liang Yuan, Ling Ma, Wei |
| author_sort | Lim, Audrey R. Q. |
| collection | NTU |
| description | Sunflower (Helianthus annuus) is one of the most important oilseed crops worldwide. However, the transcriptional regulation underlying oil accumulation in sunflower is not fully understood. WRINKLED1 (WRI1) is an essential transcription factor governing oil accumulation in plant cells. Here, we identify and characterize a sunflower ortholog of WRI1 (HaWRI1), which is highly expressed in developing seeds. Transient production of HaWRI1 stimulated substantial oil accumulation in Nicotiana benthamiana leaves. Dual-luciferase reporter assay, electrophoretic mobility shift assay, fatty acid quantification, and gene expression analysis demonstrate that HaWRI1 acts as a pivotal transcription factor controlling the expression of genes involved in late glycolysis and fatty acid biosynthesis. HaWRI1 directly binds to the cis-element, AW-box, in the promoter of biotin carboxyl carrier protein isoform 2 (BCCP2). In addition, we characterize an 80 amino-acid C-terminal domain of HaWRI1 that is crucial for transactivation. Moreover, seed-specific overexpression of HaWRI1 in Arabidopsis plants leads to enhanced seed oil content as well as upregulation of the genes involved in fatty acid biosynthesis. Taken together, our work demonstrates that HaWRI1 plays a pivotal role in the transcriptional control of seed oil accumulation, providing a potential target for bioengineering sunflower oil yield improvement. |
| first_indexed | 2024-10-01T05:30:11Z |
| format | Journal Article |
| id | ntu-10356/155728 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:30:11Z |
| publishDate | 2022 |
| record_format | dspace |
| spelling | ntu-10356/1557282023-02-28T17:09:50Z Sunflower WRINKLED1 plays a key role in transcriptional regulation of oil biosynthesis Lim, Audrey R. Q. Kong, Que Singh, Sanjay K. Guo, Liang Yuan, Ling Ma, Wei School of Biological Sciences Science::Biological sciences Gene Expression Plant Oil Biosynthesis Sunflower (Helianthus annuus) is one of the most important oilseed crops worldwide. However, the transcriptional regulation underlying oil accumulation in sunflower is not fully understood. WRINKLED1 (WRI1) is an essential transcription factor governing oil accumulation in plant cells. Here, we identify and characterize a sunflower ortholog of WRI1 (HaWRI1), which is highly expressed in developing seeds. Transient production of HaWRI1 stimulated substantial oil accumulation in Nicotiana benthamiana leaves. Dual-luciferase reporter assay, electrophoretic mobility shift assay, fatty acid quantification, and gene expression analysis demonstrate that HaWRI1 acts as a pivotal transcription factor controlling the expression of genes involved in late glycolysis and fatty acid biosynthesis. HaWRI1 directly binds to the cis-element, AW-box, in the promoter of biotin carboxyl carrier protein isoform 2 (BCCP2). In addition, we characterize an 80 amino-acid C-terminal domain of HaWRI1 that is crucial for transactivation. Moreover, seed-specific overexpression of HaWRI1 in Arabidopsis plants leads to enhanced seed oil content as well as upregulation of the genes involved in fatty acid biosynthesis. Taken together, our work demonstrates that HaWRI1 plays a pivotal role in the transcriptional control of seed oil accumulation, providing a potential target for bioengineering sunflower oil yield improvement. Ministry of Education (MOE) Published version This work was supported by the Ministry of Education (MOE) of Singapore Tier 1 to W.M. (grant number: RG29/20) and MOE of Singapore Tier 2 to W.M. (grant number: MOE-T2EP30220-0011). 2022-03-15T00:34:44Z 2022-03-15T00:34:44Z 2022 Journal Article Lim, A. R. Q., Kong, Q., Singh, S. K., Guo, L., Yuan, L. & Ma, W. (2022). Sunflower WRINKLED1 plays a key role in transcriptional regulation of oil biosynthesis. International Journal of Molecular Sciences, 23(6), 3054-. https://dx.doi.org/10.3390/ijms23063054 1661-6596 https://hdl.handle.net/10356/155728 10.3390/ijms23063054 6 23 3054 en RG29/20 MOE-T2EP30220-0011 International Journal of Molecular Sciences © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). application/pdf |
| spellingShingle | Science::Biological sciences Gene Expression Plant Oil Biosynthesis Lim, Audrey R. Q. Kong, Que Singh, Sanjay K. Guo, Liang Yuan, Ling Ma, Wei Sunflower WRINKLED1 plays a key role in transcriptional regulation of oil biosynthesis |
| title | Sunflower WRINKLED1 plays a key role in transcriptional regulation of oil biosynthesis |
| title_full | Sunflower WRINKLED1 plays a key role in transcriptional regulation of oil biosynthesis |
| title_fullStr | Sunflower WRINKLED1 plays a key role in transcriptional regulation of oil biosynthesis |
| title_full_unstemmed | Sunflower WRINKLED1 plays a key role in transcriptional regulation of oil biosynthesis |
| title_short | Sunflower WRINKLED1 plays a key role in transcriptional regulation of oil biosynthesis |
| title_sort | sunflower wrinkled1 plays a key role in transcriptional regulation of oil biosynthesis |
| topic | Science::Biological sciences Gene Expression Plant Oil Biosynthesis |
| url | https://hdl.handle.net/10356/155728 |
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