Identification and characterisation of putative probiotic for use in aquacultured fish
The aquaculture industry is turning to the use of probiotics to promote good health among fish and improve their productivity. Among the different types of probiotics, host-derived probiotics promise improved efficacy towards specific fish species, compared to commercial or terrestrial-derived pr...
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| Other Authors: | |
| Format: | Final Year Project (FYP) |
| Language: | English |
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Nanyang Technological University
2022
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| Online Access: | https://hdl.handle.net/10356/156295 |
| _version_ | 1811680459452579840 |
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| author | Lim, Chiun Hao |
| author2 | Loo Say Chye Joachim |
| author_facet | Loo Say Chye Joachim Lim, Chiun Hao |
| author_sort | Lim, Chiun Hao |
| collection | NTU |
| description | The aquaculture industry is turning to the use of probiotics to promote good health among fish
and improve their productivity. Among the different types of probiotics, host-derived
probiotics promise improved efficacy towards specific fish species, compared to commercial
or terrestrial-derived probiotics. Hence, to fully utilise the benefits of host-derived probiotics,
this study aimed to identify and characterise potential probiotic strains of fish gut origin that
can improve the disease resistance of Jade Perch, one of the newly farmed fish species in
Singapore. Potential probiotics were isolated from the gut of five adult Jade Perches and tested
for their antimicrobial properties against fish pathogens including Aeromonas sobria,
Streptococcus iniae, and Streptococcus agalactiae. The final probiotic candidate was identified
using 16S rRNA sequencing and Whole-Genome Sequencing. It was also characterised in
terms of its biosafety and viability in different harsh conditions. The isolate that can inhibit the
growth of A. sobria and S. iniae was identified to be Enterococcus hirae. It contains no
virulence genes, antibiotic resistance genes, and mobile genetic elements. E. hirae presents
good viability in environments of pH 3.0 and above, and with temperatures of 30°C to 45°C. It
is found to be viable in simulated intestinal fluid, but not in simulated gastric fluid. The use of
E. hirae as a probiotic for Jade Perch is promising as it is both safe and effective. More work
is required, however, to explore suitable encapsulation techniques to protect the probiotic
against a very low pH environment and gastric fluids. Future in vivo feeding trials are also
recommended to test for its efficacy as a probiotic for Jade Perch under natural conditions. |
| first_indexed | 2024-10-01T03:25:23Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/156295 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:25:23Z |
| publishDate | 2022 |
| publisher | Nanyang Technological University |
| record_format | dspace |
| spelling | ntu-10356/1562952022-04-14T13:43:40Z Identification and characterisation of putative probiotic for use in aquacultured fish Lim, Chiun Hao Loo Say Chye Joachim School of Materials Science and Engineering JoachimLoo@ntu.edu.sg Science::Biological sciences::Microbiology Engineering::Materials The aquaculture industry is turning to the use of probiotics to promote good health among fish and improve their productivity. Among the different types of probiotics, host-derived probiotics promise improved efficacy towards specific fish species, compared to commercial or terrestrial-derived probiotics. Hence, to fully utilise the benefits of host-derived probiotics, this study aimed to identify and characterise potential probiotic strains of fish gut origin that can improve the disease resistance of Jade Perch, one of the newly farmed fish species in Singapore. Potential probiotics were isolated from the gut of five adult Jade Perches and tested for their antimicrobial properties against fish pathogens including Aeromonas sobria, Streptococcus iniae, and Streptococcus agalactiae. The final probiotic candidate was identified using 16S rRNA sequencing and Whole-Genome Sequencing. It was also characterised in terms of its biosafety and viability in different harsh conditions. The isolate that can inhibit the growth of A. sobria and S. iniae was identified to be Enterococcus hirae. It contains no virulence genes, antibiotic resistance genes, and mobile genetic elements. E. hirae presents good viability in environments of pH 3.0 and above, and with temperatures of 30°C to 45°C. It is found to be viable in simulated intestinal fluid, but not in simulated gastric fluid. The use of E. hirae as a probiotic for Jade Perch is promising as it is both safe and effective. More work is required, however, to explore suitable encapsulation techniques to protect the probiotic against a very low pH environment and gastric fluids. Future in vivo feeding trials are also recommended to test for its efficacy as a probiotic for Jade Perch under natural conditions. Bachelor of Engineering (Materials Engineering) 2022-04-14T13:41:34Z 2022-04-14T13:41:34Z 2022 Final Year Project (FYP) Lim, C. H. (2022). Identification and characterisation of putative probiotic for use in aquacultured fish. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/156295 https://hdl.handle.net/10356/156295 en application/pdf Nanyang Technological University |
| spellingShingle | Science::Biological sciences::Microbiology Engineering::Materials Lim, Chiun Hao Identification and characterisation of putative probiotic for use in aquacultured fish |
| title | Identification and characterisation of putative probiotic for use in aquacultured fish |
| title_full | Identification and characterisation of putative probiotic for use in aquacultured fish |
| title_fullStr | Identification and characterisation of putative probiotic for use in aquacultured fish |
| title_full_unstemmed | Identification and characterisation of putative probiotic for use in aquacultured fish |
| title_short | Identification and characterisation of putative probiotic for use in aquacultured fish |
| title_sort | identification and characterisation of putative probiotic for use in aquacultured fish |
| topic | Science::Biological sciences::Microbiology Engineering::Materials |
| url | https://hdl.handle.net/10356/156295 |
| work_keys_str_mv | AT limchiunhao identificationandcharacterisationofputativeprobioticforuseinaquaculturedfish |