Investigating non-nucleoside inhibitors targeting the Zika virus non-structural protein 5 (NS5) RNA-dependent RNA polymerase (RdRp) domain
Zika virus and members of the Flaviviridae family are re-emerging pathogens that pose a significant threat to public health. The lack of a vaccine and specific treatment of has resulted in a need for the design of therapeutic treatments to target specific parts of virus replication cycles. H...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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Nanyang Technological University
2022
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| Online Access: | https://hdl.handle.net/10356/157309 |
| _version_ | 1811695062334046208 |
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| author | Zhang, Denzel Xugeng |
| author2 | Julien Lescar |
| author_facet | Julien Lescar Zhang, Denzel Xugeng |
| author_sort | Zhang, Denzel Xugeng |
| collection | NTU |
| description | Zika virus and members of the Flaviviridae family are re-emerging pathogens that pose a
significant threat to public health. The lack of a vaccine and specific treatment of has resulted
in a need for the design of therapeutic treatments to target specific parts of virus replication
cycles.
Here, the RNA dependent RNA Polymerase (RdRP) of Zika Virus was purified based on
previously established protocols. Fluorescence-based alkaline phosphatase coupled
polymerase assay (FAPA), was used to screen a library of 86 compounds generated to target
the Zika Virus NS5 RdRP. Hit compounds identified were tested for dose-response
inhibition. Finally, crystallization efforts were carried out in hopes of obtaining crystal
structures of inhibitor compounds in complex with the Zika NS5 RdRp protein. |
| first_indexed | 2024-10-01T07:17:30Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/157309 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:17:30Z |
| publishDate | 2022 |
| publisher | Nanyang Technological University |
| record_format | dspace |
| spelling | ntu-10356/1573092023-02-28T18:09:25Z Investigating non-nucleoside inhibitors targeting the Zika virus non-structural protein 5 (NS5) RNA-dependent RNA polymerase (RdRp) domain Zhang, Denzel Xugeng Julien Lescar School of Biological Sciences Nanyang Institute of Structural Biology Julien@ntu.edu.sg Science::Biological sciences Zika virus and members of the Flaviviridae family are re-emerging pathogens that pose a significant threat to public health. The lack of a vaccine and specific treatment of has resulted in a need for the design of therapeutic treatments to target specific parts of virus replication cycles. Here, the RNA dependent RNA Polymerase (RdRP) of Zika Virus was purified based on previously established protocols. Fluorescence-based alkaline phosphatase coupled polymerase assay (FAPA), was used to screen a library of 86 compounds generated to target the Zika Virus NS5 RdRP. Hit compounds identified were tested for dose-response inhibition. Finally, crystallization efforts were carried out in hopes of obtaining crystal structures of inhibitor compounds in complex with the Zika NS5 RdRp protein. Bachelor of Science in Biological Sciences 2022-05-13T07:42:26Z 2022-05-13T07:42:26Z 2022 Final Year Project (FYP) Zhang, D. X. (2022). Investigating non-nucleoside inhibitors targeting the Zika virus non-structural protein 5 (NS5) RNA-dependent RNA polymerase (RdRp) domain. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/157309 https://hdl.handle.net/10356/157309 en application/pdf Nanyang Technological University |
| spellingShingle | Science::Biological sciences Zhang, Denzel Xugeng Investigating non-nucleoside inhibitors targeting the Zika virus non-structural protein 5 (NS5) RNA-dependent RNA polymerase (RdRp) domain |
| title | Investigating non-nucleoside inhibitors targeting the Zika virus non-structural protein 5 (NS5) RNA-dependent RNA polymerase (RdRp) domain |
| title_full | Investigating non-nucleoside inhibitors targeting the Zika virus non-structural protein 5 (NS5) RNA-dependent RNA polymerase (RdRp) domain |
| title_fullStr | Investigating non-nucleoside inhibitors targeting the Zika virus non-structural protein 5 (NS5) RNA-dependent RNA polymerase (RdRp) domain |
| title_full_unstemmed | Investigating non-nucleoside inhibitors targeting the Zika virus non-structural protein 5 (NS5) RNA-dependent RNA polymerase (RdRp) domain |
| title_short | Investigating non-nucleoside inhibitors targeting the Zika virus non-structural protein 5 (NS5) RNA-dependent RNA polymerase (RdRp) domain |
| title_sort | investigating non nucleoside inhibitors targeting the zika virus non structural protein 5 ns5 rna dependent rna polymerase rdrp domain |
| topic | Science::Biological sciences |
| url | https://hdl.handle.net/10356/157309 |
| work_keys_str_mv | AT zhangdenzelxugeng investigatingnonnucleosideinhibitorstargetingthezikavirusnonstructuralprotein5ns5rnadependentrnapolymeraserdrpdomain |